Controlled Functionalization Strategy of Proteins Preserves their Structural Integrity While Binding to Nanocarriers
Abstract The use of proteins as targeting agents often requires their chemical modification for their efficient attachment to a given surface. However, no control over the protein integrity and functionality has been demonstrated to date. Chemical over‐modification causes the loss of the native stru...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2024-10-01
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| Series: | Advanced Materials Interfaces |
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| Online Access: | https://doi.org/10.1002/admi.202400472 |
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| author | Ana Mateos‐Maroto Meiyu Gai Maximilian Brückner Volker Mailänder Svenja Morsbach Katharina Landfester |
| author_facet | Ana Mateos‐Maroto Meiyu Gai Maximilian Brückner Volker Mailänder Svenja Morsbach Katharina Landfester |
| author_sort | Ana Mateos‐Maroto |
| collection | DOAJ |
| description | Abstract The use of proteins as targeting agents often requires their chemical modification for their efficient attachment to a given surface. However, no control over the protein integrity and functionality has been demonstrated to date. Chemical over‐modification causes the loss of the native structure of the protein and thus limits its targeting efficiency. To preserve structural integrity, a minimal modification strategy of proteins is developed while maintaining their functionality. Apolipoprotein A1 (ApoA1) and liposomes are utilized as a nanocarrier platform. Monitoring NHS ester chemistry by time‐of‐flight mass spectrometry experiments, the proposed functionalization route allows the effective chemical coupling of the minimally modified ApoA1 to the surface of the liposomes via a click chemistry reaction. The stability of the modified ApoA1 is ensured by analyzing the secondary structure by circular dichroism spectroscopy and the corresponding melting point by nano differential scanning fluorimetry. Furthermore, ApoA1 attachment to the liposomes is confirmed by flow cytometry experiments. The procedure presented in this study has the potential to be easily transferred to other proteins while introducing only minimally necessary chemical modifications to be covalently attached to different drug delivery platforms. This can help to improve their targeting efficiency for future biomedical applications. |
| format | Article |
| id | doaj-art-2bac7266765d4f338945a37834bc033f |
| institution | OA Journals |
| issn | 2196-7350 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Materials Interfaces |
| spelling | doaj-art-2bac7266765d4f338945a37834bc033f2025-08-20T01:54:16ZengWiley-VCHAdvanced Materials Interfaces2196-73502024-10-011130n/an/a10.1002/admi.202400472Controlled Functionalization Strategy of Proteins Preserves their Structural Integrity While Binding to NanocarriersAna Mateos‐Maroto0Meiyu Gai1Maximilian Brückner2Volker Mailänder3Svenja Morsbach4Katharina Landfester5Max Planck Institute for Polymer Research Ackermannweg 10 55128 Mainz GermanyMax Planck Institute for Polymer Research Ackermannweg 10 55128 Mainz GermanyMax Planck Institute for Polymer Research Ackermannweg 10 55128 Mainz GermanyMax Planck Institute for Polymer Research Ackermannweg 10 55128 Mainz GermanyMax Planck Institute for Polymer Research Ackermannweg 10 55128 Mainz GermanyMax Planck Institute for Polymer Research Ackermannweg 10 55128 Mainz GermanyAbstract The use of proteins as targeting agents often requires their chemical modification for their efficient attachment to a given surface. However, no control over the protein integrity and functionality has been demonstrated to date. Chemical over‐modification causes the loss of the native structure of the protein and thus limits its targeting efficiency. To preserve structural integrity, a minimal modification strategy of proteins is developed while maintaining their functionality. Apolipoprotein A1 (ApoA1) and liposomes are utilized as a nanocarrier platform. Monitoring NHS ester chemistry by time‐of‐flight mass spectrometry experiments, the proposed functionalization route allows the effective chemical coupling of the minimally modified ApoA1 to the surface of the liposomes via a click chemistry reaction. The stability of the modified ApoA1 is ensured by analyzing the secondary structure by circular dichroism spectroscopy and the corresponding melting point by nano differential scanning fluorimetry. Furthermore, ApoA1 attachment to the liposomes is confirmed by flow cytometry experiments. The procedure presented in this study has the potential to be easily transferred to other proteins while introducing only minimally necessary chemical modifications to be covalently attached to different drug delivery platforms. This can help to improve their targeting efficiency for future biomedical applications.https://doi.org/10.1002/admi.202400472apolipoproteinliposomesnanocarriersprotein functionalization |
| spellingShingle | Ana Mateos‐Maroto Meiyu Gai Maximilian Brückner Volker Mailänder Svenja Morsbach Katharina Landfester Controlled Functionalization Strategy of Proteins Preserves their Structural Integrity While Binding to Nanocarriers Advanced Materials Interfaces apolipoprotein liposomes nanocarriers protein functionalization |
| title | Controlled Functionalization Strategy of Proteins Preserves their Structural Integrity While Binding to Nanocarriers |
| title_full | Controlled Functionalization Strategy of Proteins Preserves their Structural Integrity While Binding to Nanocarriers |
| title_fullStr | Controlled Functionalization Strategy of Proteins Preserves their Structural Integrity While Binding to Nanocarriers |
| title_full_unstemmed | Controlled Functionalization Strategy of Proteins Preserves their Structural Integrity While Binding to Nanocarriers |
| title_short | Controlled Functionalization Strategy of Proteins Preserves their Structural Integrity While Binding to Nanocarriers |
| title_sort | controlled functionalization strategy of proteins preserves their structural integrity while binding to nanocarriers |
| topic | apolipoprotein liposomes nanocarriers protein functionalization |
| url | https://doi.org/10.1002/admi.202400472 |
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