DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp.
Summary: This study examines a natural consortium of halophilic archaea, comprising xylan-degrading Halorhabdus sp. SVX81, consortium cohabitant Haloferax volcanii SVX82 (formerly H. lucentense SVX82), and its DPANN ectosymbiont Ca. Nanohalococcus occultus SVXNc. Transcriptomics and targeted metabol...
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Elsevier
2025-02-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225000082 |
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| author | Oleg N. Reva Violetta La Cono Laura Marturano Francesca Crisafi Francesco Smedile Manasi Mudaliyar Debnath Ghosal Elena A. Selivanova Marina E. Ignatenko Manuel Ferrer Laura Fernandez-Lopez Mart Krupovic Michail M. Yakimov |
| author_facet | Oleg N. Reva Violetta La Cono Laura Marturano Francesca Crisafi Francesco Smedile Manasi Mudaliyar Debnath Ghosal Elena A. Selivanova Marina E. Ignatenko Manuel Ferrer Laura Fernandez-Lopez Mart Krupovic Michail M. Yakimov |
| author_sort | Oleg N. Reva |
| collection | DOAJ |
| description | Summary: This study examines a natural consortium of halophilic archaea, comprising xylan-degrading Halorhabdus sp. SVX81, consortium cohabitant Haloferax volcanii SVX82 (formerly H. lucentense SVX82), and its DPANN ectosymbiont Ca. Nanohalococcus occultus SVXNc. Transcriptomics and targeted metabolomics demonstrated that the tripartite consortium outperformed individual and the Halorhabdus sp. SVX81 with H. volcanii SVX82 bipartite cultures in xylan degradation, exhibiting a division of labor: the DPANN symbiont processed glycolysis products, while other members performed xylan depolymerization and biosynthesis of essential compounds. Electron microscopy and cryo-electron tomography revealed the formation of heterocellular biofilms interlinked by DPANN cells. The findings demonstrated that DPANN symbionts can interact directly with other members of microbial communities, which are not their primary hosts, influencing their gene expression. However, DPANN proliferation requires their primary host presence. The study highlights the collective contribution of consortium members to xylan degradation and their potential for biotechnological applications in the management of hypersaline environments. |
| format | Article |
| id | doaj-art-2b9fc5ea5dde47288e758b266c2bd7f0 |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-2b9fc5ea5dde47288e758b266c2bd7f02025-01-26T05:04:34ZengElsevieriScience2589-00422025-02-01282111749DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp.Oleg N. Reva0Violetta La Cono1Laura Marturano2Francesca Crisafi3Francesco Smedile4Manasi Mudaliyar5Debnath Ghosal6Elena A. Selivanova7Marina E. Ignatenko8Manuel Ferrer9Laura Fernandez-Lopez10Mart Krupovic11Michail M. Yakimov12Department of Biochemistry, Genetics and Microbiology, Centre for Bioinformatics and Computational Biology, University of Pretoria, 0002 Pretoria, South Africa; Corresponding authorExtreme Microbiology, Biotechnology and Astrobiology Group, Institute of Polar Sciences, ISP-CNR, 98122 Messina, ItalyExtreme Microbiology, Biotechnology and Astrobiology Group, Institute of Polar Sciences, ISP-CNR, 98122 Messina, ItalyExtreme Microbiology, Biotechnology and Astrobiology Group, Institute of Polar Sciences, ISP-CNR, 98122 Messina, ItalyExtreme Microbiology, Biotechnology and Astrobiology Group, Institute of Polar Sciences, ISP-CNR, 98122 Messina, ItalyDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, 3052 VIC, Australia; ARC Centre for Cryo-electron Microscopy of Membrane Proteins, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, 3052 VIC, AustraliaDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, 3052 VIC, Australia; ARC Centre for Cryo-electron Microscopy of Membrane Proteins, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, 3052 VIC, AustraliaOrenburg Federal Research Center, Institute for Cellular and Intracellular Symbiosis, Ural Branch of Russian Academy of Sciences, 460000 Orenburg, RussiaOrenburg Federal Research Center, Institute for Cellular and Intracellular Symbiosis, Ural Branch of Russian Academy of Sciences, 460000 Orenburg, RussiaInstituto de Catalisis y Petroleoquimica (ICP), CSIC, 28049 Madrid, SpainInstituto de Catalisis y Petroleoquimica (ICP), CSIC, 28049 Madrid, SpainInstitut Pasteur, Université Paris Cité, CNRS UMR6047, Archaeal Virology Unit, 75015 Paris, FranceExtreme Microbiology, Biotechnology and Astrobiology Group, Institute of Polar Sciences, ISP-CNR, 98122 Messina, Italy; Corresponding authorSummary: This study examines a natural consortium of halophilic archaea, comprising xylan-degrading Halorhabdus sp. SVX81, consortium cohabitant Haloferax volcanii SVX82 (formerly H. lucentense SVX82), and its DPANN ectosymbiont Ca. Nanohalococcus occultus SVXNc. Transcriptomics and targeted metabolomics demonstrated that the tripartite consortium outperformed individual and the Halorhabdus sp. SVX81 with H. volcanii SVX82 bipartite cultures in xylan degradation, exhibiting a division of labor: the DPANN symbiont processed glycolysis products, while other members performed xylan depolymerization and biosynthesis of essential compounds. Electron microscopy and cryo-electron tomography revealed the formation of heterocellular biofilms interlinked by DPANN cells. The findings demonstrated that DPANN symbionts can interact directly with other members of microbial communities, which are not their primary hosts, influencing their gene expression. However, DPANN proliferation requires their primary host presence. The study highlights the collective contribution of consortium members to xylan degradation and their potential for biotechnological applications in the management of hypersaline environments.http://www.sciencedirect.com/science/article/pii/S2589004225000082BiochemistryMicrobiologyEnvironmental Biotechnology |
| spellingShingle | Oleg N. Reva Violetta La Cono Laura Marturano Francesca Crisafi Francesco Smedile Manasi Mudaliyar Debnath Ghosal Elena A. Selivanova Marina E. Ignatenko Manuel Ferrer Laura Fernandez-Lopez Mart Krupovic Michail M. Yakimov DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp. iScience Biochemistry Microbiology Environmental Biotechnology |
| title | DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp. |
| title_full | DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp. |
| title_fullStr | DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp. |
| title_full_unstemmed | DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp. |
| title_short | DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp. |
| title_sort | dpann symbiont of haloferax volcanii accelerates xylan degradation by the non host haloarchaeon halorhabdus sp |
| topic | Biochemistry Microbiology Environmental Biotechnology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225000082 |
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