Toxicity of Macroalgae Extracts to Larvae of the Northern House Mosquito
The continuous use of synthetic insecticides to suppress mosquito larvae has detrimental impacts on the environment and human health. Finding novel and target-specific bio-insecticides has become crucial. Here, the larvicidal and genotoxic activities of different extracts from <i>Ulva lactuca&...
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MDPI AG
2024-11-01
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| author | Ahmed A. Rashed Yasmin M. Heikal Robert D. Finn Mohamed H. Bayoumy Amged El-Harairy Dina A. Refaay |
| author_facet | Ahmed A. Rashed Yasmin M. Heikal Robert D. Finn Mohamed H. Bayoumy Amged El-Harairy Dina A. Refaay |
| author_sort | Ahmed A. Rashed |
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| description | The continuous use of synthetic insecticides to suppress mosquito larvae has detrimental impacts on the environment and human health. Finding novel and target-specific bio-insecticides has become crucial. Here, the larvicidal and genotoxic activities of different extracts from <i>Ulva lactuca</i> and <i>Turbinaria ornata</i> toward <i>Culex pipiens</i> larvae were investigated. The macroalgae thalli were subjected to various solvent extractions followed by phytochemical quantification, larvicidal testing on <i>C. pipiens</i> larvae, genotoxic evaluation through comet assays, and compound characterization by gas chromatography–mass spectrometry. The methylene chloride extract from <i>U. lactuca</i> displayed the highest toxicity with LC<sub>50</sub> = 30.99 ppm, followed by the acetone extract from <i>T. ornata</i>, with LC<sub>50</sub> = 52.09 ppm after 72 h. <i>U. lactuca</i> exhibited the maximum contents of total alkaloids, total flavonoids, total terpenoids, total phenols, and total tannins with the methanol extract, while the acetone extract from <i>T. ornata</i> exhibited the maximum contents of total alkaloids, total flavonoids, total terpenoids, and total phenols. The methylene chloride extract of <i>U. lactuca</i> and the acetone extract of <i>T. ornata</i> caused significant DNA damage in larva body cells. Thus, the methylene chloride extract from <i>U. lactuca</i> and the acetone extract from <i>T. ornata</i> showed promising potential as environmentally friendly larvicides against <i>C. pipiens</i> larvae. |
| format | Article |
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| institution | DOAJ |
| issn | 2075-1729 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Life |
| spelling | doaj-art-2b9b700a9ce84772a92dfb11cf2683fa2025-08-20T02:53:41ZengMDPI AGLife2075-17292024-11-011412152710.3390/life14121527Toxicity of Macroalgae Extracts to Larvae of the Northern House MosquitoAhmed A. Rashed0Yasmin M. Heikal1Robert D. Finn2Mohamed H. Bayoumy3Amged El-Harairy4Dina A. Refaay5Economic Entomology Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, EgyptBotany Department, Faculty of Science, Mansoura University, Mansoura 35516, EgyptDepartment of Biochemistry & Medical Genetics, St. George’s International School of Medicine, Northumbria University, Newcastle upon Tyne NE1 8ST, UKEconomic Entomology Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, EgyptDepartment of Crop and Animal Sciences, Albrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Faculty of Life Sciences, Humboldt-Universität zu Berlin, Albrecht-Thaer-Weg 5, 14195 Berlin, GermanyBotany Department, Faculty of Science, Mansoura University, Mansoura 35516, EgyptThe continuous use of synthetic insecticides to suppress mosquito larvae has detrimental impacts on the environment and human health. Finding novel and target-specific bio-insecticides has become crucial. Here, the larvicidal and genotoxic activities of different extracts from <i>Ulva lactuca</i> and <i>Turbinaria ornata</i> toward <i>Culex pipiens</i> larvae were investigated. The macroalgae thalli were subjected to various solvent extractions followed by phytochemical quantification, larvicidal testing on <i>C. pipiens</i> larvae, genotoxic evaluation through comet assays, and compound characterization by gas chromatography–mass spectrometry. The methylene chloride extract from <i>U. lactuca</i> displayed the highest toxicity with LC<sub>50</sub> = 30.99 ppm, followed by the acetone extract from <i>T. ornata</i>, with LC<sub>50</sub> = 52.09 ppm after 72 h. <i>U. lactuca</i> exhibited the maximum contents of total alkaloids, total flavonoids, total terpenoids, total phenols, and total tannins with the methanol extract, while the acetone extract from <i>T. ornata</i> exhibited the maximum contents of total alkaloids, total flavonoids, total terpenoids, and total phenols. The methylene chloride extract of <i>U. lactuca</i> and the acetone extract of <i>T. ornata</i> caused significant DNA damage in larva body cells. Thus, the methylene chloride extract from <i>U. lactuca</i> and the acetone extract from <i>T. ornata</i> showed promising potential as environmentally friendly larvicides against <i>C. pipiens</i> larvae.https://www.mdpi.com/2075-1729/14/12/1527biocontrollarvicidal activity<i>Culex pipiens</i>Ulva lactuca<i>Turbinaria ornata</i>genotoxicity |
| spellingShingle | Ahmed A. Rashed Yasmin M. Heikal Robert D. Finn Mohamed H. Bayoumy Amged El-Harairy Dina A. Refaay Toxicity of Macroalgae Extracts to Larvae of the Northern House Mosquito Life biocontrol larvicidal activity <i>Culex pipiens</i> Ulva lactuca <i>Turbinaria ornata</i> genotoxicity |
| title | Toxicity of Macroalgae Extracts to Larvae of the Northern House Mosquito |
| title_full | Toxicity of Macroalgae Extracts to Larvae of the Northern House Mosquito |
| title_fullStr | Toxicity of Macroalgae Extracts to Larvae of the Northern House Mosquito |
| title_full_unstemmed | Toxicity of Macroalgae Extracts to Larvae of the Northern House Mosquito |
| title_short | Toxicity of Macroalgae Extracts to Larvae of the Northern House Mosquito |
| title_sort | toxicity of macroalgae extracts to larvae of the northern house mosquito |
| topic | biocontrol larvicidal activity <i>Culex pipiens</i> Ulva lactuca <i>Turbinaria ornata</i> genotoxicity |
| url | https://www.mdpi.com/2075-1729/14/12/1527 |
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