Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance
Introduction: The aim of this study was to determine the prevalence of Salmonella along the slaughter line and to identify possible critical control points in one slaughterhouse facility located in the city of Belgrade. Methodology: In total, 700 samples were tested: two swabs from both sides of...
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The Journal of Infection in Developing Countries
2017-09-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/9311 |
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| author | Jasna Mirko Kureljusic Marko Predrag Dmitrić Dejan Svetislav Vidanović Vlado Božidar Teodorović Branislav Ilija Kureljušić Maja Josip Velhner Neđeljko Ratko Karabasil |
| author_facet | Jasna Mirko Kureljusic Marko Predrag Dmitrić Dejan Svetislav Vidanović Vlado Božidar Teodorović Branislav Ilija Kureljušić Maja Josip Velhner Neđeljko Ratko Karabasil |
| author_sort | Jasna Mirko Kureljusic |
| collection | DOAJ |
| description |
Introduction: The aim of this study was to determine the prevalence of Salmonella along the slaughter line and to identify possible critical control points in one slaughterhouse facility located in the city of Belgrade.
Methodology: In total, 700 samples were tested: two swabs from both sides of carcass were taken from each of 100 pigs. In this way, 200 pig skin swab samples were taken after stunning, 200 after processing and 200 after chilling. Additional 100 samples of ileal contents were also taken from the same pigs to obtain a collection of 270 isolates. All samples were analyzed using standard culture methods and serotyping. PFGE was performed for 27 isolates. Determination of antimicrobial resistance was performed by E-test.
Results: In total, 47 (23.5%) swab samples were positive for the presence of Salmonella after stunning. After processing, Salmonella was isolated in two swab samples (1%), whereas all samples which were collected after chilling were negative for Salmonella. The sampling of ileal contents was positive for five Salmonella isolates (5%). The most frequently isolated serotypes were S. Derby (90.74%), S. Infantis (5.56%) and S. Typhimurium (3.7%). All tested isolates were resistant to tetracycline. Resistance was recorded to nalidixic acid (23.3%), ciprofloxacin (20%), ampicillin (10%) and chloramphenicol (14.4%), as well. The PFGE results indicated that isolates had a high genetic similarity.
Conclusions: The investigation has confirmed that bacteriological examinations of carcass swabs, as well as ileal content, could be used to assess the carriage of salmonellae in pigs at the time of slaughter.
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| format | Article |
| id | doaj-art-2b8e79a2ca46429885e0f4b98a6bfbbb |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2017-09-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-2b8e79a2ca46429885e0f4b98a6bfbbb2025-08-20T02:27:23ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802017-09-01110810.3855/jidc.9311Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistanceJasna Mirko Kureljusic0Marko Predrag Dmitrić1Dejan Svetislav Vidanović2Vlado Božidar Teodorović3Branislav Ilija Kureljušić4Maja Josip Velhner5Neđeljko Ratko Karabasil6Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaVeterinary Specialized Institute “Kraljevo”, Kraljevo, SerbiaVeterinary Specialized Institute “Kraljevo“, Kraljevo, SerbiaFaculty of Veterinary Medicine, University of Belgrade, Belgrade, SerbiaInstitute of Veterinary Medicine of Serbia, Belgrade, SerbiaScientific Veterinary Institute „Novi Sad“, Novi Sad, SerbiaFaculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia Introduction: The aim of this study was to determine the prevalence of Salmonella along the slaughter line and to identify possible critical control points in one slaughterhouse facility located in the city of Belgrade. Methodology: In total, 700 samples were tested: two swabs from both sides of carcass were taken from each of 100 pigs. In this way, 200 pig skin swab samples were taken after stunning, 200 after processing and 200 after chilling. Additional 100 samples of ileal contents were also taken from the same pigs to obtain a collection of 270 isolates. All samples were analyzed using standard culture methods and serotyping. PFGE was performed for 27 isolates. Determination of antimicrobial resistance was performed by E-test. Results: In total, 47 (23.5%) swab samples were positive for the presence of Salmonella after stunning. After processing, Salmonella was isolated in two swab samples (1%), whereas all samples which were collected after chilling were negative for Salmonella. The sampling of ileal contents was positive for five Salmonella isolates (5%). The most frequently isolated serotypes were S. Derby (90.74%), S. Infantis (5.56%) and S. Typhimurium (3.7%). All tested isolates were resistant to tetracycline. Resistance was recorded to nalidixic acid (23.3%), ciprofloxacin (20%), ampicillin (10%) and chloramphenicol (14.4%), as well. The PFGE results indicated that isolates had a high genetic similarity. Conclusions: The investigation has confirmed that bacteriological examinations of carcass swabs, as well as ileal content, could be used to assess the carriage of salmonellae in pigs at the time of slaughter. https://jidc.org/index.php/journal/article/view/9311pigspigSalmonellaslaughterantimicrobial resistancePFGE |
| spellingShingle | Jasna Mirko Kureljusic Marko Predrag Dmitrić Dejan Svetislav Vidanović Vlado Božidar Teodorović Branislav Ilija Kureljušić Maja Josip Velhner Neđeljko Ratko Karabasil Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance Journal of Infection in Developing Countries pigs pig Salmonella slaughter antimicrobial resistance PFGE |
| title | Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance |
| title_full | Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance |
| title_fullStr | Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance |
| title_full_unstemmed | Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance |
| title_short | Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance |
| title_sort | prevalence of salmonella enterica in slaughtered pigs in serbia serotyping pfge genotyping and antimicrobial resistance |
| topic | pigs pig Salmonella slaughter antimicrobial resistance PFGE |
| url | https://jidc.org/index.php/journal/article/view/9311 |
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