Significance of myeloid-derived suppressor cells (MDSCs) like CD14+B7-H4 cells frequency in blood and tumor microcirculation of lung cancer patients
Background/Aim. Myeloid-derived suppressor cells (MDSCs) suppress immune responses via a series of inhibitory mechanisms, which ultimately could lead to tumor growth. B7-H4 expression is significantly associated with poor outcome and promotion of tumor cell proliferation, invasion and migration in p...
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Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2021-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900106V.pdf |
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| author | Vuković Jelena Karličić Vukojica Ristić Saša Stanojević Ivan Nikolić Nevena Štefik Debra Jović Milena Abazović Džihan Šupić Gordana Vojvodić Danilo Pavlović Miloš |
| author_facet | Vuković Jelena Karličić Vukojica Ristić Saša Stanojević Ivan Nikolić Nevena Štefik Debra Jović Milena Abazović Džihan Šupić Gordana Vojvodić Danilo Pavlović Miloš |
| author_sort | Vuković Jelena |
| collection | DOAJ |
| description | Background/Aim. Myeloid-derived suppressor cells (MDSCs) suppress immune responses via a series of inhibitory mechanisms, which ultimately could lead to tumor growth. B7-H4 expression is significantly associated with poor outcome and promotion of tumor cell proliferation, invasion and migration in patients with various cancers. Data concerning B7-H4 expression in lung cancers (LC), either on tumor or immunological cells, are still sporadic. The aim was to estimate and correlate the number of CD14+B7-H4+MDSCs in blood and lung tumor microcirculation with clinical stage, histology type of tumor, tumor node metastasis (TNM) stadium, nodal status and disease outspread. Methods. The study included 44 lung cancer patients (III and IV clinical stage) and 30 healthy controls. CD14+B7-H4+ MDSC number was estimated by flow cytometry in blood and tumor microcirculation samples of each patient. Results. CD14+B7-H4+ MDSCs number was significantly higher in patients’ samples compared to controls. CD14+B7-H4+ MDSC number was significantly increased in tumor compared to blood sample of the same patient. Clinical stage III patients had the increased number of the CD14+B7-H4+ MDSC compared to stage IV, in both types of samples. According to histology, small cell lung cancer (SCLC) patients had the highest average CD14+B7-H4+ MDSCs number, significantly increased compared to patients with squamous and large cell LC histology type. Tumor size was directly associated with the number of the CD14+B7-H4+ MDSC, both in blood and tumor samples. Furthermore, nodal involvement was associated with the gradual increase of the CD14+B7-H4+ MDSC number, being the highest in the N3 group, again both in blood and tumor samples. Finally, we detected higher CD14+B7-H4+ MDSCs number in the samples of patients without metastases. Conclusion. CD14+B7-H4+ MDSCs n umber in L C patients is significantly associated with tumor histology type, lymph node involvement, disease extent degree and tumor size. Concerning their large number in LC tumor microenvironment together with immunosuppressive capacities, CD14+B7-H4+ MDSCs could represent important tumor promoting factor in LC pathophysiology. |
| format | Article |
| id | doaj-art-2b8e565df62e4dc5ac59f259f1dcbef1 |
| institution | OA Journals |
| issn | 0042-8450 2406-0720 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-2b8e565df62e4dc5ac59f259f1dcbef12025-08-20T02:04:31ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502406-07202021-01-0178659960610.2298/VSP190430106V0042-84501900106VSignificance of myeloid-derived suppressor cells (MDSCs) like CD14+B7-H4 cells frequency in blood and tumor microcirculation of lung cancer patientsVuković Jelena0Karličić Vukojica1Ristić Saša2Stanojević Ivan3Nikolić Nevena4Štefik Debra5Jović Milena6Abazović Džihan7Šupić Gordana8https://orcid.org/0000-0003-0350-4733Vojvodić Danilo9Pavlović Miloš10Military Medical Academy, Clinic for Pulmonology, Belgrade, SerbiaEuromedik Special Hospital, Belgrade, SerbiaInstitute of Pathology and Forensic Medicine, Belgrade, SerbiaInstitute for Medical Research, Belgrade, Serbia + University of Defence, Faculty of Medicine of the Military Medical Academy, Serbia, BelgradeUniversity of Defence, Faculty of Medicine of the Military Medical Academy, Serbia, BelgradeRenova Center for Regenerative Medicine, Belgrade, SerbiaEuromedik Special Hospital, Belgrade, SerbiaRenova Center for Regenerative Medicine, Belgrade, SerbiaInstitute for Medical Research, Belgrade, Serbia + University of Defence, Faculty of Medicine of the Military Medical Academy, Serbia, BelgradeInstitute for Medical Research, Belgrade, Serbia + University of Defence, Faculty of Medicine of the Military Medical Academy, Serbia, BelgradeUniversity of Belgrade, Faculty of Veterinary Medicine, Belgrade, SerbiaBackground/Aim. Myeloid-derived suppressor cells (MDSCs) suppress immune responses via a series of inhibitory mechanisms, which ultimately could lead to tumor growth. B7-H4 expression is significantly associated with poor outcome and promotion of tumor cell proliferation, invasion and migration in patients with various cancers. Data concerning B7-H4 expression in lung cancers (LC), either on tumor or immunological cells, are still sporadic. The aim was to estimate and correlate the number of CD14+B7-H4+MDSCs in blood and lung tumor microcirculation with clinical stage, histology type of tumor, tumor node metastasis (TNM) stadium, nodal status and disease outspread. Methods. The study included 44 lung cancer patients (III and IV clinical stage) and 30 healthy controls. CD14+B7-H4+ MDSC number was estimated by flow cytometry in blood and tumor microcirculation samples of each patient. Results. CD14+B7-H4+ MDSCs number was significantly higher in patients’ samples compared to controls. CD14+B7-H4+ MDSC number was significantly increased in tumor compared to blood sample of the same patient. Clinical stage III patients had the increased number of the CD14+B7-H4+ MDSC compared to stage IV, in both types of samples. According to histology, small cell lung cancer (SCLC) patients had the highest average CD14+B7-H4+ MDSCs number, significantly increased compared to patients with squamous and large cell LC histology type. Tumor size was directly associated with the number of the CD14+B7-H4+ MDSC, both in blood and tumor samples. Furthermore, nodal involvement was associated with the gradual increase of the CD14+B7-H4+ MDSC number, being the highest in the N3 group, again both in blood and tumor samples. Finally, we detected higher CD14+B7-H4+ MDSCs number in the samples of patients without metastases. Conclusion. CD14+B7-H4+ MDSCs n umber in L C patients is significantly associated with tumor histology type, lymph node involvement, disease extent degree and tumor size. Concerning their large number in LC tumor microenvironment together with immunosuppressive capacities, CD14+B7-H4+ MDSCs could represent important tumor promoting factor in LC pathophysiology.http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900106V.pdflung neoplasmsmyeloid-derived suppressor cellsimmunologic factorsneoplasm metastasisflow cytometryhistology |
| spellingShingle | Vuković Jelena Karličić Vukojica Ristić Saša Stanojević Ivan Nikolić Nevena Štefik Debra Jović Milena Abazović Džihan Šupić Gordana Vojvodić Danilo Pavlović Miloš Significance of myeloid-derived suppressor cells (MDSCs) like CD14+B7-H4 cells frequency in blood and tumor microcirculation of lung cancer patients Vojnosanitetski Pregled lung neoplasms myeloid-derived suppressor cells immunologic factors neoplasm metastasis flow cytometry histology |
| title | Significance of myeloid-derived suppressor cells (MDSCs) like CD14+B7-H4 cells frequency in blood and tumor microcirculation of lung cancer patients |
| title_full | Significance of myeloid-derived suppressor cells (MDSCs) like CD14+B7-H4 cells frequency in blood and tumor microcirculation of lung cancer patients |
| title_fullStr | Significance of myeloid-derived suppressor cells (MDSCs) like CD14+B7-H4 cells frequency in blood and tumor microcirculation of lung cancer patients |
| title_full_unstemmed | Significance of myeloid-derived suppressor cells (MDSCs) like CD14+B7-H4 cells frequency in blood and tumor microcirculation of lung cancer patients |
| title_short | Significance of myeloid-derived suppressor cells (MDSCs) like CD14+B7-H4 cells frequency in blood and tumor microcirculation of lung cancer patients |
| title_sort | significance of myeloid derived suppressor cells mdscs like cd14 b7 h4 cells frequency in blood and tumor microcirculation of lung cancer patients |
| topic | lung neoplasms myeloid-derived suppressor cells immunologic factors neoplasm metastasis flow cytometry histology |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900106V.pdf |
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