Enhanced Plant Growth Through Composite Inoculation of Phosphate-Solubilizing Bacteria: Insights from Plate and Soil Experiments
Excessive application of phosphorus (P) fertilizers does not alleviate P deficiency in soils and may cause water eutrophication. The available P in acidic soils is bound to minerals, such as iron and aluminum, in forms that are difficult to utilize by plants. The low availability of P is detrimental...
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2024-10-01
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| author | Mengsha Li Xinjing Li Daosheng Xue Chengjiang Bao Keying Zhang Lili Chen Qiuping Li Rui Guo |
| author_facet | Mengsha Li Xinjing Li Daosheng Xue Chengjiang Bao Keying Zhang Lili Chen Qiuping Li Rui Guo |
| author_sort | Mengsha Li |
| collection | DOAJ |
| description | Excessive application of phosphorus (P) fertilizers does not alleviate P deficiency in soils and may cause water eutrophication. The available P in acidic soils is bound to minerals, such as iron and aluminum, in forms that are difficult to utilize by plants. The low availability of P is detrimental to soil health and crop growth. To address the P imbalance in the soil, different bioremediation techniques, such as phosphate-solubilizing bacteria (PSB) application, have been employed. However, the systematic analysis of the effects of composite inoculation of PSB on crops remains elusive. In this study, the effects of composite-inoculated PSB on plant growth were systematically evaluated by two scales: plate experiment and soil test. This study employed six different strains of PSB including <i>Lelliottia amnigena</i> 1-1 (A), <i>Kluyvera intermedia</i> 1-2 (B), <i>Pseudomonas tolaasii</i> 1-6 (C), <i>Burkholderia cepacia</i> 2-5 (D), <i>Pseudomonas frederiksbergensis</i> 2-11 (E), and <i>Pseudomonas rhodesiae</i> 2-47 (F). Among the 57 different combinations of these strains, four combinations (AE, AF, ADF, and AEF) indicated higher phosphate-solubilizing abilities than the single strains. These combinations were used for subsequent experiments. The plate experiment revealed that composite strains were more effective than single strains in promoting the growth and development of seedlings and roots of oilseed rape. Furthermore, AE, AF, and AEF combinations indicated excellent growth-promoting effects. Moreover, the soil test revealed that the composite inoculation of AE and AEF significantly enhanced biomass accumulation and root development in oilseed rape. The increased growth-promoting effects of the composite strains were observed to be associated with to their phosphate-solubilizing capacities. Both scales confirmed that compared to single inoculation, composite inoculation of PSB is more beneficial for plant growth. This study provides composite inoculation materials and foundational data to support the bioremediation of P imbalance in soil. |
| format | Article |
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| language | English |
| publishDate | 2024-10-01 |
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| series | Agronomy |
| spelling | doaj-art-2b882f620a854b7fad56c3df2126b8af2025-08-20T02:08:00ZengMDPI AGAgronomy2073-43952024-10-011411246110.3390/agronomy14112461Enhanced Plant Growth Through Composite Inoculation of Phosphate-Solubilizing Bacteria: Insights from Plate and Soil ExperimentsMengsha Li0Xinjing Li1Daosheng Xue2Chengjiang Bao3Keying Zhang4Lili Chen5Qiuping Li6Rui Guo7Ningbo Key Laboratory of Agricultural Germplasm Resources Mining and Environmental Regulation, College of Science and Technology, Ningbo University, Cixi 315300, ChinaNingbo Key Laboratory of Agricultural Germplasm Resources Mining and Environmental Regulation, College of Science and Technology, Ningbo University, Cixi 315300, ChinaNingbo Key Laboratory of Agricultural Germplasm Resources Mining and Environmental Regulation, College of Science and Technology, Ningbo University, Cixi 315300, ChinaNingbo Key Laboratory of Agricultural Germplasm Resources Mining and Environmental Regulation, College of Science and Technology, Ningbo University, Cixi 315300, ChinaNingbo Key Laboratory of Agricultural Germplasm Resources Mining and Environmental Regulation, College of Science and Technology, Ningbo University, Cixi 315300, ChinaNingbo Key Laboratory of Agricultural Germplasm Resources Mining and Environmental Regulation, College of Science and Technology, Ningbo University, Cixi 315300, ChinaNingbo Key Laboratory of Agricultural Germplasm Resources Mining and Environmental Regulation, College of Science and Technology, Ningbo University, Cixi 315300, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaExcessive application of phosphorus (P) fertilizers does not alleviate P deficiency in soils and may cause water eutrophication. The available P in acidic soils is bound to minerals, such as iron and aluminum, in forms that are difficult to utilize by plants. The low availability of P is detrimental to soil health and crop growth. To address the P imbalance in the soil, different bioremediation techniques, such as phosphate-solubilizing bacteria (PSB) application, have been employed. However, the systematic analysis of the effects of composite inoculation of PSB on crops remains elusive. In this study, the effects of composite-inoculated PSB on plant growth were systematically evaluated by two scales: plate experiment and soil test. This study employed six different strains of PSB including <i>Lelliottia amnigena</i> 1-1 (A), <i>Kluyvera intermedia</i> 1-2 (B), <i>Pseudomonas tolaasii</i> 1-6 (C), <i>Burkholderia cepacia</i> 2-5 (D), <i>Pseudomonas frederiksbergensis</i> 2-11 (E), and <i>Pseudomonas rhodesiae</i> 2-47 (F). Among the 57 different combinations of these strains, four combinations (AE, AF, ADF, and AEF) indicated higher phosphate-solubilizing abilities than the single strains. These combinations were used for subsequent experiments. The plate experiment revealed that composite strains were more effective than single strains in promoting the growth and development of seedlings and roots of oilseed rape. Furthermore, AE, AF, and AEF combinations indicated excellent growth-promoting effects. Moreover, the soil test revealed that the composite inoculation of AE and AEF significantly enhanced biomass accumulation and root development in oilseed rape. The increased growth-promoting effects of the composite strains were observed to be associated with to their phosphate-solubilizing capacities. Both scales confirmed that compared to single inoculation, composite inoculation of PSB is more beneficial for plant growth. This study provides composite inoculation materials and foundational data to support the bioremediation of P imbalance in soil.https://www.mdpi.com/2073-4395/14/11/2461phosphate-solubilizing bacteriacomposite inoculationoilseed rapegrowth promotionbioremediation of phosphorus imbalance |
| spellingShingle | Mengsha Li Xinjing Li Daosheng Xue Chengjiang Bao Keying Zhang Lili Chen Qiuping Li Rui Guo Enhanced Plant Growth Through Composite Inoculation of Phosphate-Solubilizing Bacteria: Insights from Plate and Soil Experiments Agronomy phosphate-solubilizing bacteria composite inoculation oilseed rape growth promotion bioremediation of phosphorus imbalance |
| title | Enhanced Plant Growth Through Composite Inoculation of Phosphate-Solubilizing Bacteria: Insights from Plate and Soil Experiments |
| title_full | Enhanced Plant Growth Through Composite Inoculation of Phosphate-Solubilizing Bacteria: Insights from Plate and Soil Experiments |
| title_fullStr | Enhanced Plant Growth Through Composite Inoculation of Phosphate-Solubilizing Bacteria: Insights from Plate and Soil Experiments |
| title_full_unstemmed | Enhanced Plant Growth Through Composite Inoculation of Phosphate-Solubilizing Bacteria: Insights from Plate and Soil Experiments |
| title_short | Enhanced Plant Growth Through Composite Inoculation of Phosphate-Solubilizing Bacteria: Insights from Plate and Soil Experiments |
| title_sort | enhanced plant growth through composite inoculation of phosphate solubilizing bacteria insights from plate and soil experiments |
| topic | phosphate-solubilizing bacteria composite inoculation oilseed rape growth promotion bioremediation of phosphorus imbalance |
| url | https://www.mdpi.com/2073-4395/14/11/2461 |
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