The inhibitory and anti-inflammatory effects of TMP269 on peste des petits ruminants virus replication
Peste des petits ruminants (PPR) is an acute and fatal contagious disease, caused by the PPR virus (PPRV), and is one of the most damaging animal diseases. The replication of many viruses is closely related to the regulation of histone deacetylases (HDACs). TMP269, a selective class IIa HDAC inhibit...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Virulence |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2025.2495838 |
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| author | Miaomiao Li Yang Wang Qunhua Ke Min Qu Xuelian Meng Yuefeng Sun |
| author_facet | Miaomiao Li Yang Wang Qunhua Ke Min Qu Xuelian Meng Yuefeng Sun |
| author_sort | Miaomiao Li |
| collection | DOAJ |
| description | Peste des petits ruminants (PPR) is an acute and fatal contagious disease, caused by the PPR virus (PPRV), and is one of the most damaging animal diseases. The replication of many viruses is closely related to the regulation of histone deacetylases (HDACs). TMP269, a selective class IIa HDAC inhibitor, plays an important role in cancer therapy and also modulates viral replication. However, the regulatory effects of TMP269 on PPRV replication remain poorly understood. In this study, we employed western blotting, quantitative Real-time PCR (qRT-PCR), RNA sequencing (RNA-seq), and enzyme-linked immunosorbent assay (ELISA) to evaluate the inhibitory and anti-inflammatory effects of TMP269 on PPRV replication. Western blot analysis showed that TMP269 treatment significantly suppressed PPRV replication in Vero and caprine endometrial epithelial cells (EECs). RNA-seq data revealed that the upregulation of inflammatory response genes induced by PPRV infection was markedly reversed by TMP269. Further, qRT-PCR and ELISA demonstrated that TMP269 decreased the expression of the pro-inflammatory chemokines CCL2, CCL5, CCL7, CXCL8, and cytokine IL-6 during infection, suggesting the vital role of TMP269 in anti-inflammatory processes. Collectively, our findings suggest that the class IIa HDAC inhibitor TMP269 is a promising antiviral agent for PPRV and provides novel insights into the antiviral and anti-inflammatory abilities of TMP269. |
| format | Article |
| id | doaj-art-2b877ffaf2214dc2ab7f523dccb327a9 |
| institution | DOAJ |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj-art-2b877ffaf2214dc2ab7f523dccb327a92025-08-20T03:14:14ZengTaylor & Francis GroupVirulence2150-55942150-56082025-12-0116110.1080/21505594.2025.2495838The inhibitory and anti-inflammatory effects of TMP269 on peste des petits ruminants virus replicationMiaomiao Li0Yang Wang1Qunhua Ke2Min Qu3Xuelian Meng4Yuefeng Sun5State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medicine, Lanzhou University, Lanzhou, ChinaState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaPeste des petits ruminants (PPR) is an acute and fatal contagious disease, caused by the PPR virus (PPRV), and is one of the most damaging animal diseases. The replication of many viruses is closely related to the regulation of histone deacetylases (HDACs). TMP269, a selective class IIa HDAC inhibitor, plays an important role in cancer therapy and also modulates viral replication. However, the regulatory effects of TMP269 on PPRV replication remain poorly understood. In this study, we employed western blotting, quantitative Real-time PCR (qRT-PCR), RNA sequencing (RNA-seq), and enzyme-linked immunosorbent assay (ELISA) to evaluate the inhibitory and anti-inflammatory effects of TMP269 on PPRV replication. Western blot analysis showed that TMP269 treatment significantly suppressed PPRV replication in Vero and caprine endometrial epithelial cells (EECs). RNA-seq data revealed that the upregulation of inflammatory response genes induced by PPRV infection was markedly reversed by TMP269. Further, qRT-PCR and ELISA demonstrated that TMP269 decreased the expression of the pro-inflammatory chemokines CCL2, CCL5, CCL7, CXCL8, and cytokine IL-6 during infection, suggesting the vital role of TMP269 in anti-inflammatory processes. Collectively, our findings suggest that the class IIa HDAC inhibitor TMP269 is a promising antiviral agent for PPRV and provides novel insights into the antiviral and anti-inflammatory abilities of TMP269.https://www.tandfonline.com/doi/10.1080/21505594.2025.2495838PPRVTMP269RNA sequencinginflammatory responses |
| spellingShingle | Miaomiao Li Yang Wang Qunhua Ke Min Qu Xuelian Meng Yuefeng Sun The inhibitory and anti-inflammatory effects of TMP269 on peste des petits ruminants virus replication Virulence PPRV TMP269 RNA sequencing inflammatory responses |
| title | The inhibitory and anti-inflammatory effects of TMP269 on peste des petits ruminants virus replication |
| title_full | The inhibitory and anti-inflammatory effects of TMP269 on peste des petits ruminants virus replication |
| title_fullStr | The inhibitory and anti-inflammatory effects of TMP269 on peste des petits ruminants virus replication |
| title_full_unstemmed | The inhibitory and anti-inflammatory effects of TMP269 on peste des petits ruminants virus replication |
| title_short | The inhibitory and anti-inflammatory effects of TMP269 on peste des petits ruminants virus replication |
| title_sort | inhibitory and anti inflammatory effects of tmp269 on peste des petits ruminants virus replication |
| topic | PPRV TMP269 RNA sequencing inflammatory responses |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2025.2495838 |
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