Substrate preference of protein kinase B isoforms can vary depending on the cell line.
Many proteins in higher eukaryotes, especially those with crucial functions, have multiple isoforms with redundant roles providing protection against potential functional deficiencies in one isoform. However, these isoforms can also have some unique roles. Protein kinase B, also known as Akt, is one...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0298322 |
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| _version_ | 1850105956519641088 |
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| author | Miguel S Palma Samantha R Perez Aida Husain Deepali Bhandari |
| author_facet | Miguel S Palma Samantha R Perez Aida Husain Deepali Bhandari |
| author_sort | Miguel S Palma |
| collection | DOAJ |
| description | Many proteins in higher eukaryotes, especially those with crucial functions, have multiple isoforms with redundant roles providing protection against potential functional deficiencies in one isoform. However, these isoforms can also have some unique roles. Protein kinase B, also known as Akt, is one such protein that has three isoforms encoded on different genes. Due to high sequence similarity and the general lack of specific reagents, most studies on Akt generalize their findings and do not distinguish between the isoforms. Using an established chemical genetic strategy and a set of known Akt substrates, this work explores substrate specificity of Akt isoforms under steady state conditions in two commonly used cell lines. This strategy can be applied to study any Akt isoform-specific substrates of interest in any cell line of choice as long as the cell line can be transfected. |
| format | Article |
| id | doaj-art-2b85535fcf5b42a5b4992fecf0fb817b |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-2b85535fcf5b42a5b4992fecf0fb817b2025-08-20T02:38:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01193e029832210.1371/journal.pone.0298322Substrate preference of protein kinase B isoforms can vary depending on the cell line.Miguel S PalmaSamantha R PerezAida HusainDeepali BhandariMany proteins in higher eukaryotes, especially those with crucial functions, have multiple isoforms with redundant roles providing protection against potential functional deficiencies in one isoform. However, these isoforms can also have some unique roles. Protein kinase B, also known as Akt, is one such protein that has three isoforms encoded on different genes. Due to high sequence similarity and the general lack of specific reagents, most studies on Akt generalize their findings and do not distinguish between the isoforms. Using an established chemical genetic strategy and a set of known Akt substrates, this work explores substrate specificity of Akt isoforms under steady state conditions in two commonly used cell lines. This strategy can be applied to study any Akt isoform-specific substrates of interest in any cell line of choice as long as the cell line can be transfected.https://doi.org/10.1371/journal.pone.0298322 |
| spellingShingle | Miguel S Palma Samantha R Perez Aida Husain Deepali Bhandari Substrate preference of protein kinase B isoforms can vary depending on the cell line. PLoS ONE |
| title | Substrate preference of protein kinase B isoforms can vary depending on the cell line. |
| title_full | Substrate preference of protein kinase B isoforms can vary depending on the cell line. |
| title_fullStr | Substrate preference of protein kinase B isoforms can vary depending on the cell line. |
| title_full_unstemmed | Substrate preference of protein kinase B isoforms can vary depending on the cell line. |
| title_short | Substrate preference of protein kinase B isoforms can vary depending on the cell line. |
| title_sort | substrate preference of protein kinase b isoforms can vary depending on the cell line |
| url | https://doi.org/10.1371/journal.pone.0298322 |
| work_keys_str_mv | AT miguelspalma substratepreferenceofproteinkinasebisoformscanvarydependingonthecellline AT samantharperez substratepreferenceofproteinkinasebisoformscanvarydependingonthecellline AT aidahusain substratepreferenceofproteinkinasebisoformscanvarydependingonthecellline AT deepalibhandari substratepreferenceofproteinkinasebisoformscanvarydependingonthecellline |