Transcriptomic characteristics of bronchoalveolar lavage fluid and peripheral blood mononuclear cells in COVID-19 patients
Circulating in China and 158 other countries and areas, the ongoing COVID-19 outbreak has caused devastating mortality and posed a great threat to public health. However, efforts to identify effectively supportive therapeutic drugs and treatments has been hampered by our limited understanding of hos...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1747363 |
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| author | Yong Xiong Yuan Liu Liu Cao Dehe Wang Ming Guo Ao Jiang Dong Guo Wenjia Hu Jiayi Yang Zhidong Tang Honglong Wu Yongquan Lin Meiyuan Zhang Qi Zhang Mang Shi Yingle Liu Yu Zhou Ke Lan Yu Chen |
| author_facet | Yong Xiong Yuan Liu Liu Cao Dehe Wang Ming Guo Ao Jiang Dong Guo Wenjia Hu Jiayi Yang Zhidong Tang Honglong Wu Yongquan Lin Meiyuan Zhang Qi Zhang Mang Shi Yingle Liu Yu Zhou Ke Lan Yu Chen |
| author_sort | Yong Xiong |
| collection | DOAJ |
| description | Circulating in China and 158 other countries and areas, the ongoing COVID-19 outbreak has caused devastating mortality and posed a great threat to public health. However, efforts to identify effectively supportive therapeutic drugs and treatments has been hampered by our limited understanding of host immune response for this fatal disease. To characterize the transcriptional signatures of host inflammatory response to SARS-CoV-2 (HCoV-19) infection, we carried out transcriptome sequencing of the RNAs isolated from the bronchoalveolar lavage fluid (BALF) and peripheral blood mononuclear cells (PBMC) specimens of COVID-19 patients. Our results reveal distinct host inflammatory cytokine profiles to SARS-CoV-2 infection in patients, and highlight the association between COVID-19 pathogenesis and excessive cytokine release such as CCL2/MCP-1, CXCL10/IP-10, CCL3/MIP-1A, and CCL4/MIP1B. Furthermore, SARS-CoV-2 induced activation of apoptosis and P53 signalling pathway in lymphocytes may be the cause of patients’ lymphopenia. The transcriptome dataset of COVID-19 patients would be a valuable resource for clinical guidance on anti-inflammatory medication and understanding the molecular mechansims of host response. |
| format | Article |
| id | doaj-art-2b8451e5e713432b89a487080b46bcf4 |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-2b8451e5e713432b89a487080b46bcf42025-08-20T02:26:36ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-019176177010.1080/22221751.2020.1747363Transcriptomic characteristics of bronchoalveolar lavage fluid and peripheral blood mononuclear cells in COVID-19 patientsYong Xiong0Yuan Liu1Liu Cao2Dehe Wang3Ming Guo4Ao Jiang5Dong Guo6Wenjia Hu7Jiayi Yang8Zhidong Tang9Honglong Wu10Yongquan Lin11Meiyuan Zhang12Qi Zhang13Mang Shi14Yingle Liu15Yu Zhou16Ke Lan17Yu Chen18State Key Laboratory of Virology, Department of Infectious Disease, Zhongnan Hospital, Wuhan University, Wuhan, People’s Republic of ChinaState Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of ChinaThe Centre for Infection and Immunity Studies, School of Medicine, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of ChinaState Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of ChinaState Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of ChinaState Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of ChinaState Key Laboratory of Virology, Department of Infectious Disease, Zhongnan Hospital, Wuhan University, Wuhan, People’s Republic of ChinaState Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of ChinaState Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of ChinaBGI PathoGenesis Pharmaceutical Technology, Shenzhen, People’s Republic of ChinaBGI PathoGenesis Pharmaceutical Technology, Shenzhen, People’s Republic of ChinaBGI PathoGenesis Pharmaceutical Technology, Shenzhen, People’s Republic of ChinaState Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of ChinaThe Centre for Infection and Immunity Studies, School of Medicine, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of ChinaState Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of ChinaState Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of ChinaState Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of ChinaCirculating in China and 158 other countries and areas, the ongoing COVID-19 outbreak has caused devastating mortality and posed a great threat to public health. However, efforts to identify effectively supportive therapeutic drugs and treatments has been hampered by our limited understanding of host immune response for this fatal disease. To characterize the transcriptional signatures of host inflammatory response to SARS-CoV-2 (HCoV-19) infection, we carried out transcriptome sequencing of the RNAs isolated from the bronchoalveolar lavage fluid (BALF) and peripheral blood mononuclear cells (PBMC) specimens of COVID-19 patients. Our results reveal distinct host inflammatory cytokine profiles to SARS-CoV-2 infection in patients, and highlight the association between COVID-19 pathogenesis and excessive cytokine release such as CCL2/MCP-1, CXCL10/IP-10, CCL3/MIP-1A, and CCL4/MIP1B. Furthermore, SARS-CoV-2 induced activation of apoptosis and P53 signalling pathway in lymphocytes may be the cause of patients’ lymphopenia. The transcriptome dataset of COVID-19 patients would be a valuable resource for clinical guidance on anti-inflammatory medication and understanding the molecular mechansims of host response.https://www.tandfonline.com/doi/10.1080/22221751.2020.1747363COVID-19transcriptome profilinginflammationcytokinelymphopenia |
| spellingShingle | Yong Xiong Yuan Liu Liu Cao Dehe Wang Ming Guo Ao Jiang Dong Guo Wenjia Hu Jiayi Yang Zhidong Tang Honglong Wu Yongquan Lin Meiyuan Zhang Qi Zhang Mang Shi Yingle Liu Yu Zhou Ke Lan Yu Chen Transcriptomic characteristics of bronchoalveolar lavage fluid and peripheral blood mononuclear cells in COVID-19 patients Emerging Microbes and Infections COVID-19 transcriptome profiling inflammation cytokine lymphopenia |
| title | Transcriptomic characteristics of bronchoalveolar lavage fluid and peripheral blood mononuclear cells in COVID-19 patients |
| title_full | Transcriptomic characteristics of bronchoalveolar lavage fluid and peripheral blood mononuclear cells in COVID-19 patients |
| title_fullStr | Transcriptomic characteristics of bronchoalveolar lavage fluid and peripheral blood mononuclear cells in COVID-19 patients |
| title_full_unstemmed | Transcriptomic characteristics of bronchoalveolar lavage fluid and peripheral blood mononuclear cells in COVID-19 patients |
| title_short | Transcriptomic characteristics of bronchoalveolar lavage fluid and peripheral blood mononuclear cells in COVID-19 patients |
| title_sort | transcriptomic characteristics of bronchoalveolar lavage fluid and peripheral blood mononuclear cells in covid 19 patients |
| topic | COVID-19 transcriptome profiling inflammation cytokine lymphopenia |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1747363 |
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