Genotypic characterization of Egypt enterotoxigenic Escherichia coli isolates expressing coli surface antigen 6
Introduction: One approach to control enterotoxigenic Escherichia coli (ETEC) infections has been to develop vaccines focused on inducing protective immunity against surface expressed antigenic factors. One such factor is coli surface antigen 6 (CS6); ETEC isolates expressing CS6 may also simultane...
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The Journal of Infection in Developing Countries
2013-02-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/2454 |
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| author | Atef M El-Gendy Adel Mansour Hind I Shaheen Marshall R Monteville Adam W Armstrong Nasr El-Sayed Sylvia Y. N Young John D Klena |
| author_facet | Atef M El-Gendy Adel Mansour Hind I Shaheen Marshall R Monteville Adam W Armstrong Nasr El-Sayed Sylvia Y. N Young John D Klena |
| author_sort | Atef M El-Gendy |
| collection | DOAJ |
| description |
Introduction: One approach to control enterotoxigenic Escherichia coli (ETEC) infections has been to develop vaccines focused on inducing protective immunity against surface expressed antigenic factors. One such factor is coli surface antigen 6 (CS6); ETEC isolates expressing CS6 may also simultaneously co-express surface antigens CS4 or CS5. However, there is little information regarding the inter-relationships of isolates expressing the CS6 antigen alone or in combination with CS4 or CS5.
Methodology: A total of 62 CS6-associated ETEC isolates were evaluated for their antimicrobial susceptibility, mechanisms of resistance, toxin genes, colonization factor expression, and XbaI-pulsed-field gel electrophoretic profiles.
Results: We observed 46 XbaI profiles; 31 were exclusive to ETEC expressing CS6 alone and 15 among the ETEC co-expressing CS4 or CS5. Nearly half (47%) of these isolates were resistant to ampicillin, a third (37%) of the isolates were resistant to trimethoprim-sulfamethoxazole, and 24% of the isolates were tetracycline-resistant. A blaTEM gene was detected in 24 (83%) ampicillin-resistant isolates. Trimethoprim-sulfamethoxazole-resistant isolates (n = 23) carried either sulI (n = 1, 4%), sulII (n = 8, 35%) or both genes (n = 10, 43%); 4 had no detectable sul gene.
Conclusions: Our results show a lack of clonality among Egypt CS6 E. coli isolates and supports the use and the further research on vaccines targeting this cell surface antigen.
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| format | Article |
| id | doaj-art-2b8316473b884a3ca4a0a10a089be024 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2013-02-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-2b8316473b884a3ca4a0a10a089be0242025-08-20T03:48:58ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802013-02-0170210.3855/jidc.2454Genotypic characterization of Egypt enterotoxigenic Escherichia coli isolates expressing coli surface antigen 6Atef M El-Gendy0Adel Mansour1Hind I Shaheen2Marshall R Monteville3Adam W Armstrong4Nasr El-Sayed5Sylvia Y. N Young6John D Klena7US Naval Medical Research Unit No. 3, Cairo, EgyptUS Naval Medical Research Unit No. 3, Cairo, EgyptUS Naval Medical Research Unit No. 3, Cairo, EgyptUS Naval Medical Research Unit No. 3, Cairo, EgyptUS Naval Medical Research Unit No. 3, Cairo, EgyptEgyptian Ministry of Health, Cairo, Egypt.US Naval Medical Research Unit No. 3, Cairo, EgyptUS Naval Medical Research Unit No. 3, Cairo, Egypt Introduction: One approach to control enterotoxigenic Escherichia coli (ETEC) infections has been to develop vaccines focused on inducing protective immunity against surface expressed antigenic factors. One such factor is coli surface antigen 6 (CS6); ETEC isolates expressing CS6 may also simultaneously co-express surface antigens CS4 or CS5. However, there is little information regarding the inter-relationships of isolates expressing the CS6 antigen alone or in combination with CS4 or CS5. Methodology: A total of 62 CS6-associated ETEC isolates were evaluated for their antimicrobial susceptibility, mechanisms of resistance, toxin genes, colonization factor expression, and XbaI-pulsed-field gel electrophoretic profiles. Results: We observed 46 XbaI profiles; 31 were exclusive to ETEC expressing CS6 alone and 15 among the ETEC co-expressing CS4 or CS5. Nearly half (47%) of these isolates were resistant to ampicillin, a third (37%) of the isolates were resistant to trimethoprim-sulfamethoxazole, and 24% of the isolates were tetracycline-resistant. A blaTEM gene was detected in 24 (83%) ampicillin-resistant isolates. Trimethoprim-sulfamethoxazole-resistant isolates (n = 23) carried either sulI (n = 1, 4%), sulII (n = 8, 35%) or both genes (n = 10, 43%); 4 had no detectable sul gene. Conclusions: Our results show a lack of clonality among Egypt CS6 E. coli isolates and supports the use and the further research on vaccines targeting this cell surface antigen. https://jidc.org/index.php/journal/article/view/2454Enterotoxigenic Escherichia colichildrendiarrheaEgypt |
| spellingShingle | Atef M El-Gendy Adel Mansour Hind I Shaheen Marshall R Monteville Adam W Armstrong Nasr El-Sayed Sylvia Y. N Young John D Klena Genotypic characterization of Egypt enterotoxigenic Escherichia coli isolates expressing coli surface antigen 6 Journal of Infection in Developing Countries Enterotoxigenic Escherichia coli children diarrhea Egypt |
| title | Genotypic characterization of Egypt enterotoxigenic Escherichia coli isolates expressing coli surface antigen 6 |
| title_full | Genotypic characterization of Egypt enterotoxigenic Escherichia coli isolates expressing coli surface antigen 6 |
| title_fullStr | Genotypic characterization of Egypt enterotoxigenic Escherichia coli isolates expressing coli surface antigen 6 |
| title_full_unstemmed | Genotypic characterization of Egypt enterotoxigenic Escherichia coli isolates expressing coli surface antigen 6 |
| title_short | Genotypic characterization of Egypt enterotoxigenic Escherichia coli isolates expressing coli surface antigen 6 |
| title_sort | genotypic characterization of egypt enterotoxigenic escherichia coli isolates expressing coli surface antigen 6 |
| topic | Enterotoxigenic Escherichia coli children diarrhea Egypt |
| url | https://jidc.org/index.php/journal/article/view/2454 |
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