Emergence of extended-spectrum beta-lactamases-producing Escherichia coli in community-acquired urinary infections in Casablanca, Morocco
Introduction: Extended-spectrum beta-lactamase- (ESBL)-producing Escherichia coli are an increasingly significant cause of community-acquired infection worldwide. The aim of this study was to assess the prevalence of ESBL-producing E. coli in a community, to analyze the relationship between strains...
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The Journal of Infection in Developing Countries
2011-11-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/1490 |
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| author | Fatna Bourjilat Brahim Bouchrif Noureddine Dersi Jean David Perrier Gros Claude Hamid Amarouch Mohammed Timinouni |
| author_facet | Fatna Bourjilat Brahim Bouchrif Noureddine Dersi Jean David Perrier Gros Claude Hamid Amarouch Mohammed Timinouni |
| author_sort | Fatna Bourjilat |
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Introduction: Extended-spectrum beta-lactamase- (ESBL)-producing Escherichia coli are an increasingly significant cause of community-acquired infection worldwide. The aim of this study was to assess the prevalence of ESBL-producing E. coli in a community, to analyze the relationship between strains studied, and to characterize the ESBL genes involved in this resistance.
Methodology: ESBL production was detected by the double disk synergy test. Genes encoding ESBLs (blaTEM, blaCTM, blaSHV) were identified by PCR and DNA sequencing. Conjugation experiments were performed to check the transferability of antibiotic resistance genes. Strain inter-relationships were studied by pulsed field gel electrophoresis.
Results: Seven ESBL-producing E. coli were identified among the 535 E. coli isolates. Most of them expressed a CTX-M enzyme (6/7) with a predominance of CTX-M-15 (6/6). Two strains possessed TEM in combination with CTX-M-15 or SHV-5. Plasmid content and gene transfer analysis showed that resistance genes were carried by high molecular weight conjugative plasmids. PFGE analysis showed that the strains were not clonal.
Conclusions: ESBL-producing E. coli from urinary tract infections in Casablanca belong to different clones and carry mobile beta-lactamase genes. It is therefore essential to monitor the epidemiology of ESBLs in E. coli and related organisms locally to effectively combat resistance.
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| format | Article |
| id | doaj-art-2b7f0bcb382c4b6b85ee01e2387e10cf |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2011-11-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-2b7f0bcb382c4b6b85ee01e2387e10cf2025-08-20T03:48:58ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802011-11-0151210.3855/jidc.1490Emergence of extended-spectrum beta-lactamases-producing Escherichia coli in community-acquired urinary infections in Casablanca, MoroccoFatna Bourjilat0Brahim Bouchrif1Noureddine Dersi2Jean David Perrier Gros Claude3Hamid Amarouch4Mohammed Timinouni5Laboratory of Microbiology, Medical Biology Center, Pasteur Institute of Morocco, Casablanca, MoroccoLaboratory of Microbiology, Medical Biology Center, Pasteur Institute of Morocco, Casablanca, MoroccoLaboratory of Microbiology, Medical Biology Center, Pasteur Institute of Morocco, Casablanca, MoroccoLaboratoire of Molecular Bacteriology, Pasteur Institute of Morocco, Casablanca, MoroccoLaboratory of Microbiology, Faculty of Sciences, Ain chok, Casablanca, MoroccoLaboratoire of Molecular Bacteriology, Pasteur Institute of Morocco, Casablanca, Morocco Introduction: Extended-spectrum beta-lactamase- (ESBL)-producing Escherichia coli are an increasingly significant cause of community-acquired infection worldwide. The aim of this study was to assess the prevalence of ESBL-producing E. coli in a community, to analyze the relationship between strains studied, and to characterize the ESBL genes involved in this resistance. Methodology: ESBL production was detected by the double disk synergy test. Genes encoding ESBLs (blaTEM, blaCTM, blaSHV) were identified by PCR and DNA sequencing. Conjugation experiments were performed to check the transferability of antibiotic resistance genes. Strain inter-relationships were studied by pulsed field gel electrophoresis. Results: Seven ESBL-producing E. coli were identified among the 535 E. coli isolates. Most of them expressed a CTX-M enzyme (6/7) with a predominance of CTX-M-15 (6/6). Two strains possessed TEM in combination with CTX-M-15 or SHV-5. Plasmid content and gene transfer analysis showed that resistance genes were carried by high molecular weight conjugative plasmids. PFGE analysis showed that the strains were not clonal. Conclusions: ESBL-producing E. coli from urinary tract infections in Casablanca belong to different clones and carry mobile beta-lactamase genes. It is therefore essential to monitor the epidemiology of ESBLs in E. coli and related organisms locally to effectively combat resistance. https://jidc.org/index.php/journal/article/view/1490urinary tract infectionEscherichia colicommunityESBLPFGEMorocco |
| spellingShingle | Fatna Bourjilat Brahim Bouchrif Noureddine Dersi Jean David Perrier Gros Claude Hamid Amarouch Mohammed Timinouni Emergence of extended-spectrum beta-lactamases-producing Escherichia coli in community-acquired urinary infections in Casablanca, Morocco Journal of Infection in Developing Countries urinary tract infection Escherichia coli community ESBL PFGE Morocco |
| title | Emergence of extended-spectrum beta-lactamases-producing Escherichia coli in community-acquired urinary infections in Casablanca, Morocco |
| title_full | Emergence of extended-spectrum beta-lactamases-producing Escherichia coli in community-acquired urinary infections in Casablanca, Morocco |
| title_fullStr | Emergence of extended-spectrum beta-lactamases-producing Escherichia coli in community-acquired urinary infections in Casablanca, Morocco |
| title_full_unstemmed | Emergence of extended-spectrum beta-lactamases-producing Escherichia coli in community-acquired urinary infections in Casablanca, Morocco |
| title_short | Emergence of extended-spectrum beta-lactamases-producing Escherichia coli in community-acquired urinary infections in Casablanca, Morocco |
| title_sort | emergence of extended spectrum beta lactamases producing escherichia coli in community acquired urinary infections in casablanca morocco |
| topic | urinary tract infection Escherichia coli community ESBL PFGE Morocco |
| url | https://jidc.org/index.php/journal/article/view/1490 |
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