Isolation and Characterization of Bacillus thuringiensis Isolates from Hazelnut Orchards in Türkiye
The entomopathogenic bacterium, Bacillus thuringiensis (Bt) has been one of the important sources of biopesticides and applied in biological control against agricultural pests for many decades. Isolation and characterization of Bt isolates from different habitats around the world allow the discovery...
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Hasan Eleroğlu
2025-04-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | https://agrifoodscience.com/index.php/TURJAF/article/view/7415 |
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| author | Burcu Şahin Hatice Güneş |
| author_facet | Burcu Şahin Hatice Güneş |
| author_sort | Burcu Şahin |
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| description | The entomopathogenic bacterium, Bacillus thuringiensis (Bt) has been one of the important sources of biopesticides and applied in biological control against agricultural pests for many decades. Isolation and characterization of Bt isolates from different habitats around the world allow the discovery of new Bt strains with high insecticidal activity and the ability to cope with the problem of resistance to pesticides. The goal of this study is to obtain a new Bt collection from hazelnut orchards in Türkiye and to investigate the insecticidal cry1 gene content of these isolates and to reveal their protein profiles and serovars. Bt was isolated from 28 soil and leaf samples collected from 14 different locations in Ordu province. Of the 302 Bt-like colonies examined, 63 were observed to carry crystals in the form of parasporal inclusions and the Bt index was found to be 0.21. The presence of cry1 gene in Bt isolates were analyzed using Polymerase chain reaction (PCR) analysis. The results showed that 21 isolates (33%) were positive for cry1 gene. When the protein profiles of the Bt isolates were examined by SDS-PAGE analysis, bands of 130 kDa, 60 kDa, 43 kDa and larger than 200 kDa were obtained in most of the samples. In order to reveal the serovar types of Bt isolates, hag gene was amplified and the PCR products were further sequenced. The analysis identified the Bt tochigiensis and Bt xiaguangiensis serovars among the screened Bt isolates. This study reports the isolation and characterization of a novel collection of B. thuringiensis isolates from hazelnut orchards in Ordu province (Northern Türkiye), identifying the cry1 gene carrying strains with potential toxicity against some lepidopteran pests. |
| format | Article |
| id | doaj-art-2b7a550ef48440dca2438d01c525ce63 |
| institution | DOAJ |
| issn | 2148-127X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Hasan Eleroğlu |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-2b7a550ef48440dca2438d01c525ce632025-08-20T03:17:52ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2025-04-0113499199710.24925/turjaf.v13i4.991-997.74156116Isolation and Characterization of Bacillus thuringiensis Isolates from Hazelnut Orchards in TürkiyeBurcu Şahin0https://orcid.org/0000-0002-5701-1371Hatice Güneş1https://orcid.org/0000-0001-5191-365XMuğla Sıtkı Koçman University, Faculty of Science, Department of Biology, 48000, Muğla, TürkiyeMuğla Sıtkı Koçman University, Faculty of Science, Department of Biology, 48000, Muğla, TürkiyeThe entomopathogenic bacterium, Bacillus thuringiensis (Bt) has been one of the important sources of biopesticides and applied in biological control against agricultural pests for many decades. Isolation and characterization of Bt isolates from different habitats around the world allow the discovery of new Bt strains with high insecticidal activity and the ability to cope with the problem of resistance to pesticides. The goal of this study is to obtain a new Bt collection from hazelnut orchards in Türkiye and to investigate the insecticidal cry1 gene content of these isolates and to reveal their protein profiles and serovars. Bt was isolated from 28 soil and leaf samples collected from 14 different locations in Ordu province. Of the 302 Bt-like colonies examined, 63 were observed to carry crystals in the form of parasporal inclusions and the Bt index was found to be 0.21. The presence of cry1 gene in Bt isolates were analyzed using Polymerase chain reaction (PCR) analysis. The results showed that 21 isolates (33%) were positive for cry1 gene. When the protein profiles of the Bt isolates were examined by SDS-PAGE analysis, bands of 130 kDa, 60 kDa, 43 kDa and larger than 200 kDa were obtained in most of the samples. In order to reveal the serovar types of Bt isolates, hag gene was amplified and the PCR products were further sequenced. The analysis identified the Bt tochigiensis and Bt xiaguangiensis serovars among the screened Bt isolates. This study reports the isolation and characterization of a novel collection of B. thuringiensis isolates from hazelnut orchards in Ordu province (Northern Türkiye), identifying the cry1 gene carrying strains with potential toxicity against some lepidopteran pests.https://agrifoodscience.com/index.php/TURJAF/article/view/7415bacillus thuringiensisbt indexcry1 genesds-pagehag gene |
| spellingShingle | Burcu Şahin Hatice Güneş Isolation and Characterization of Bacillus thuringiensis Isolates from Hazelnut Orchards in Türkiye Turkish Journal of Agriculture: Food Science and Technology bacillus thuringiensis bt index cry1 gene sds-page hag gene |
| title | Isolation and Characterization of Bacillus thuringiensis Isolates from Hazelnut Orchards in Türkiye |
| title_full | Isolation and Characterization of Bacillus thuringiensis Isolates from Hazelnut Orchards in Türkiye |
| title_fullStr | Isolation and Characterization of Bacillus thuringiensis Isolates from Hazelnut Orchards in Türkiye |
| title_full_unstemmed | Isolation and Characterization of Bacillus thuringiensis Isolates from Hazelnut Orchards in Türkiye |
| title_short | Isolation and Characterization of Bacillus thuringiensis Isolates from Hazelnut Orchards in Türkiye |
| title_sort | isolation and characterization of bacillus thuringiensis isolates from hazelnut orchards in turkiye |
| topic | bacillus thuringiensis bt index cry1 gene sds-page hag gene |
| url | https://agrifoodscience.com/index.php/TURJAF/article/view/7415 |
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