Simple and Selective HPLC-UV/Vis Bioanalytical Method to Determine Aluminum Phthalocyanine Chloride in Skin Permeation Studies
Considering the feasibility of the aluminum phthalocyanine chloride (AlPcCl) application in the topical photodynamic therapy of cutaneous tumors and the lack of HPLC methods capable of supporting skin permeation experiments using this compound, the aim of this study was to obtain a simple and select...
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| Format: | Article |
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Wiley
2018-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/7423764 |
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| author | Thaiene Avila Reis Ana Elise Jaculi Rubens da Costa Alves Tais Gratieri Ricardo Bentes Azevedo Graziella Anselmo Joanitti Guilherme Martins Gelfuso Marcilio Cunha-Filho |
| author_facet | Thaiene Avila Reis Ana Elise Jaculi Rubens da Costa Alves Tais Gratieri Ricardo Bentes Azevedo Graziella Anselmo Joanitti Guilherme Martins Gelfuso Marcilio Cunha-Filho |
| author_sort | Thaiene Avila Reis |
| collection | DOAJ |
| description | Considering the feasibility of the aluminum phthalocyanine chloride (AlPcCl) application in the topical photodynamic therapy of cutaneous tumors and the lack of HPLC methods capable of supporting skin permeation experiments using this compound, the aim of this study was to obtain a simple and selective chromatographic method for AlPcCl determination in skin matrices. A HPLC-UV/Vis method was developed using a normal-phase column operating at 30°C, an isocratic mobile phase of methanol : phosphoric acid (0.01 M) at 1.5 mL/min, and detection at 670 nm. The method exhibited (i) selectivity against various contaminants found in the different skin layers, (ii) high drug extraction capacity from the hair follicle (>70%) and remaining skin (>80%), and (iii) low limits of detection and of quantification (0.03 and 0.09 μg/mL, resp.). The method was also linear in the range from 0.1 to 5.0 µg/mL (r = 0.9994) and demonstrated robustness with regard to experimental chromatographic parameters according to a factorial design. Lastly, the developed method was successfully tested in in vitro skin permeation studies of AlPcCl, proving its effectiveness in the development of pharmaceutical delivery systems containing this drug for topical photodynamic therapy of skin cancers. |
| format | Article |
| id | doaj-art-2b6e9e50f6414fe9befba656da2d42e2 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-2b6e9e50f6414fe9befba656da2d42e22025-02-03T01:07:13ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/74237647423764Simple and Selective HPLC-UV/Vis Bioanalytical Method to Determine Aluminum Phthalocyanine Chloride in Skin Permeation StudiesThaiene Avila Reis0Ana Elise Jaculi1Rubens da Costa Alves2Tais Gratieri3Ricardo Bentes Azevedo4Graziella Anselmo Joanitti5Guilherme Martins Gelfuso6Marcilio Cunha-Filho7Laboratory of Food, Drugs and Cosmetics (LTMAC), School of Health Sciences, University of Brasília, 70.910-900 Brasília, DF, BrazilLaboratory of Food, Drugs and Cosmetics (LTMAC), School of Health Sciences, University of Brasília, 70.910-900 Brasília, DF, BrazilLaboratory of Food, Drugs and Cosmetics (LTMAC), School of Health Sciences, University of Brasília, 70.910-900 Brasília, DF, BrazilLaboratory of Food, Drugs and Cosmetics (LTMAC), School of Health Sciences, University of Brasília, 70.910-900 Brasília, DF, BrazilDepartment of Genetics and Morphology, Institute of Biological Sciences, University of Brasília, 70.910-900 Brasília, DF, BrazilFaculty of Ceilandia, University of Brasília, 72.220-900 Brasília, DF, BrazilLaboratory of Food, Drugs and Cosmetics (LTMAC), School of Health Sciences, University of Brasília, 70.910-900 Brasília, DF, BrazilLaboratory of Food, Drugs and Cosmetics (LTMAC), School of Health Sciences, University of Brasília, 70.910-900 Brasília, DF, BrazilConsidering the feasibility of the aluminum phthalocyanine chloride (AlPcCl) application in the topical photodynamic therapy of cutaneous tumors and the lack of HPLC methods capable of supporting skin permeation experiments using this compound, the aim of this study was to obtain a simple and selective chromatographic method for AlPcCl determination in skin matrices. A HPLC-UV/Vis method was developed using a normal-phase column operating at 30°C, an isocratic mobile phase of methanol : phosphoric acid (0.01 M) at 1.5 mL/min, and detection at 670 nm. The method exhibited (i) selectivity against various contaminants found in the different skin layers, (ii) high drug extraction capacity from the hair follicle (>70%) and remaining skin (>80%), and (iii) low limits of detection and of quantification (0.03 and 0.09 μg/mL, resp.). The method was also linear in the range from 0.1 to 5.0 µg/mL (r = 0.9994) and demonstrated robustness with regard to experimental chromatographic parameters according to a factorial design. Lastly, the developed method was successfully tested in in vitro skin permeation studies of AlPcCl, proving its effectiveness in the development of pharmaceutical delivery systems containing this drug for topical photodynamic therapy of skin cancers.http://dx.doi.org/10.1155/2018/7423764 |
| spellingShingle | Thaiene Avila Reis Ana Elise Jaculi Rubens da Costa Alves Tais Gratieri Ricardo Bentes Azevedo Graziella Anselmo Joanitti Guilherme Martins Gelfuso Marcilio Cunha-Filho Simple and Selective HPLC-UV/Vis Bioanalytical Method to Determine Aluminum Phthalocyanine Chloride in Skin Permeation Studies Journal of Analytical Methods in Chemistry |
| title | Simple and Selective HPLC-UV/Vis Bioanalytical Method to Determine Aluminum Phthalocyanine Chloride in Skin Permeation Studies |
| title_full | Simple and Selective HPLC-UV/Vis Bioanalytical Method to Determine Aluminum Phthalocyanine Chloride in Skin Permeation Studies |
| title_fullStr | Simple and Selective HPLC-UV/Vis Bioanalytical Method to Determine Aluminum Phthalocyanine Chloride in Skin Permeation Studies |
| title_full_unstemmed | Simple and Selective HPLC-UV/Vis Bioanalytical Method to Determine Aluminum Phthalocyanine Chloride in Skin Permeation Studies |
| title_short | Simple and Selective HPLC-UV/Vis Bioanalytical Method to Determine Aluminum Phthalocyanine Chloride in Skin Permeation Studies |
| title_sort | simple and selective hplc uv vis bioanalytical method to determine aluminum phthalocyanine chloride in skin permeation studies |
| url | http://dx.doi.org/10.1155/2018/7423764 |
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