Comparative Analysis of the Effects of Crude Metabolic Extracts of Three Biocontrol Bacteria on Microbial Community Structure Provides a New Strategy for the Biological Control of Apple Replant Disease
The crude metabolic extract from plant biocontrol bacteria plays a very important role in sustainable agricultural production. These extracts help maintain healthy plants and have very important application prospects in biotechnology related to alleviating apple replant disease (ARD). In this study,...
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MDPI AG
2024-09-01
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| Series: | Horticulturae |
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| author | Jinhui Lv Weitao Jiang Zihui Xu Gongshuai Wang Xiaoxuan Li Xinyu Wu Fengxia Ding Yusong Liu Xuesen Chen Chengmiao Yin Zhiquan Mao |
| author_facet | Jinhui Lv Weitao Jiang Zihui Xu Gongshuai Wang Xiaoxuan Li Xinyu Wu Fengxia Ding Yusong Liu Xuesen Chen Chengmiao Yin Zhiquan Mao |
| author_sort | Jinhui Lv |
| collection | DOAJ |
| description | The crude metabolic extract from plant biocontrol bacteria plays a very important role in sustainable agricultural production. These extracts help maintain healthy plants and have very important application prospects in biotechnology related to alleviating apple replant disease (ARD). In this study, <i>Bacillus velezensis</i> XC1 (T1), <i>Bacillus amyloliquefaciens</i> QSB-6 (T2), and <i>Lactobacillus reuteri</i> LBR (T3) were examined to characterize the ability of their crude metabolic extracts to alleviate ARD. The high-throughput sequencing data of the soil microbial community structure were analyzed in relation to LBR crude metabolic extracts, and an extensive untargeted metabolomic analysis of UHPLC-Qex active components was performed. Active LC-MS/MS revealed that the main secondary metabolites involved in the biological control exerted by <i>L. reuteri</i> included 3-hydroxypropionaldehyde, extracellular polysaccharides (EPS), p-hydroxybenzoic acid, and azelaic acid. These crude metabolic extracts significantly inhibited the growth of soil pathogenic fungi, reduced the abundance of <i>Fusarium</i>, promoted the abundance of beneficial bacteria such as <i>Pseudomonas</i>, and optimized the soil microbial community structure. Improved modern extraction and purification technologies will be able to offer additional insights into the mechanism of action of these secondary metabolites and enable them to be used in biological preparations to prevent and control ARD in the future, as well as to allow harmful chemical fumigants to be discontinued. |
| format | Article |
| id | doaj-art-2b6e0b15b03e4945a21da2b3f118b770 |
| institution | OA Journals |
| issn | 2311-7524 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
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| series | Horticulturae |
| spelling | doaj-art-2b6e0b15b03e4945a21da2b3f118b7702025-08-20T02:11:12ZengMDPI AGHorticulturae2311-75242024-09-011010103510.3390/horticulturae10101035Comparative Analysis of the Effects of Crude Metabolic Extracts of Three Biocontrol Bacteria on Microbial Community Structure Provides a New Strategy for the Biological Control of Apple Replant DiseaseJinhui Lv0Weitao Jiang1Zihui Xu2Gongshuai Wang3Xiaoxuan Li4Xinyu Wu5Fengxia Ding6Yusong Liu7Xuesen Chen8Chengmiao Yin9Zhiquan Mao10College of Horticulture Science, Engineering Shandong Agricultural University, Tai’an 271018, ChinaCollege of Horticulture Science, Engineering Shandong Agricultural University, Tai’an 271018, ChinaCollege of Horticulture Science, Engineering Shandong Agricultural University, Tai’an 271018, ChinaCollege of Agricultural Science and Technology, Shandong Agriculture and Engineering University, Jinan 251100, ChinaCollege of Horticulture Science, Engineering Shandong Agricultural University, Tai’an 271018, ChinaCollege of Horticulture Science, Engineering Shandong Agricultural University, Tai’an 271018, ChinaWudi County Jieshi Shan Town Agricultural Comprehensive Service Center, Binzhou 251910, ChinaCollege of Horticulture Science, Engineering Shandong Agricultural University, Tai’an 271018, ChinaCollege of Horticulture Science, Engineering Shandong Agricultural University, Tai’an 271018, ChinaCollege of Horticulture Science, Engineering Shandong Agricultural University, Tai’an 271018, ChinaCollege of Horticulture Science, Engineering Shandong Agricultural University, Tai’an 271018, ChinaThe crude metabolic extract from plant biocontrol bacteria plays a very important role in sustainable agricultural production. These extracts help maintain healthy plants and have very important application prospects in biotechnology related to alleviating apple replant disease (ARD). In this study, <i>Bacillus velezensis</i> XC1 (T1), <i>Bacillus amyloliquefaciens</i> QSB-6 (T2), and <i>Lactobacillus reuteri</i> LBR (T3) were examined to characterize the ability of their crude metabolic extracts to alleviate ARD. The high-throughput sequencing data of the soil microbial community structure were analyzed in relation to LBR crude metabolic extracts, and an extensive untargeted metabolomic analysis of UHPLC-Qex active components was performed. Active LC-MS/MS revealed that the main secondary metabolites involved in the biological control exerted by <i>L. reuteri</i> included 3-hydroxypropionaldehyde, extracellular polysaccharides (EPS), p-hydroxybenzoic acid, and azelaic acid. These crude metabolic extracts significantly inhibited the growth of soil pathogenic fungi, reduced the abundance of <i>Fusarium</i>, promoted the abundance of beneficial bacteria such as <i>Pseudomonas</i>, and optimized the soil microbial community structure. Improved modern extraction and purification technologies will be able to offer additional insights into the mechanism of action of these secondary metabolites and enable them to be used in biological preparations to prevent and control ARD in the future, as well as to allow harmful chemical fumigants to be discontinued.https://www.mdpi.com/2311-7524/10/10/1035appleapple replant disease<i>Lactobacillus reuteri</i>crude metabolic extractmicrobial community structure |
| spellingShingle | Jinhui Lv Weitao Jiang Zihui Xu Gongshuai Wang Xiaoxuan Li Xinyu Wu Fengxia Ding Yusong Liu Xuesen Chen Chengmiao Yin Zhiquan Mao Comparative Analysis of the Effects of Crude Metabolic Extracts of Three Biocontrol Bacteria on Microbial Community Structure Provides a New Strategy for the Biological Control of Apple Replant Disease Horticulturae apple apple replant disease <i>Lactobacillus reuteri</i> crude metabolic extract microbial community structure |
| title | Comparative Analysis of the Effects of Crude Metabolic Extracts of Three Biocontrol Bacteria on Microbial Community Structure Provides a New Strategy for the Biological Control of Apple Replant Disease |
| title_full | Comparative Analysis of the Effects of Crude Metabolic Extracts of Three Biocontrol Bacteria on Microbial Community Structure Provides a New Strategy for the Biological Control of Apple Replant Disease |
| title_fullStr | Comparative Analysis of the Effects of Crude Metabolic Extracts of Three Biocontrol Bacteria on Microbial Community Structure Provides a New Strategy for the Biological Control of Apple Replant Disease |
| title_full_unstemmed | Comparative Analysis of the Effects of Crude Metabolic Extracts of Three Biocontrol Bacteria on Microbial Community Structure Provides a New Strategy for the Biological Control of Apple Replant Disease |
| title_short | Comparative Analysis of the Effects of Crude Metabolic Extracts of Three Biocontrol Bacteria on Microbial Community Structure Provides a New Strategy for the Biological Control of Apple Replant Disease |
| title_sort | comparative analysis of the effects of crude metabolic extracts of three biocontrol bacteria on microbial community structure provides a new strategy for the biological control of apple replant disease |
| topic | apple apple replant disease <i>Lactobacillus reuteri</i> crude metabolic extract microbial community structure |
| url | https://www.mdpi.com/2311-7524/10/10/1035 |
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