Overcoming challenges in metagenomic AMR surveillance with nanopore sequencing: a case study on fluoroquinolone resistance

Overcoming challenges in metagenomic AMR surveillance with nanopore sequencing: a case study on fluoroquinolone resistance

IntroductionAntimicrobial resistance is an alarming public health problem, and comprehensive surveillance across environments is required to reduce its impact. Phenotypic testing and whole-genome sequencing of isolates are efficient, but culture-free approaches like metagenomic sequencing potentiall...

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Bibliographic Details
Main Authors: Bram Bloemen, Mathieu Gand, Moniek Ringenier, Bert Bogaerts, Kevin Vanneste, Kathleen Marchal, Nancy H. C. Roosens, Jeroen Dewulf, Filip Boyen, Sigrid C. J. De Keersmaecker
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Microbiology
Subjects:
metagenomic sequencing
antimicrobial resistance (AMR)
nanopore sequencing
DNA methylation
strain-resolved metagenomics
plasmid host prediction
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1614301/full
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