Effects of stretch on chondrogenic differentiation of rat condylar fibrocartilage stem cells
[Objective:] To investigate the effect of mechanical stretch on the chondrogenic differentiation of rat condylar fibrocartilage stem cells (FCSCs). [Methods:] Triple-lineage induction was used to verify the multi-lineage differentiation ability of rat condylar FCSCs, and flow cytometry was conducted...
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Editorial Office of Journal of Oral and Maxillofacial Surgery
2025-02-01
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| Series: | Kouqiang hemian waike zazhi |
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| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2025.01.003 |
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| _version_ | 1849225010238980096 |
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| author | GONG Wuyi MI Xiaohui LI Yongming |
| author_facet | GONG Wuyi MI Xiaohui LI Yongming |
| author_sort | GONG Wuyi |
| collection | DOAJ |
| description | [Objective:] To investigate the effect of mechanical stretch on the chondrogenic differentiation of rat condylar fibrocartilage stem cells (FCSCs). [Methods:] Triple-lineage induction was used to verify the multi-lineage differentiation ability of rat condylar FCSCs, and flow cytometry was conducted to examine the surface markers of rat condylar FCSCs. Afterward, the rat condylar FCSCs were stretched with a strength of 10% for 48 h, and the expression of related chondrogenic genes was detected by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting. We also established rat mandibular advancement (MA) model to find out the in vivo effect of mechanical stretch on the superficial layer of the condylar cartilage. [Results:] The rat condylar FCSCs could differentiate towards adipogenesis, osteogenesis, and chondrogenesis, and they expressed mesenchymal markers CD90 and CD29, while negative for hemopoietic markers CD45 and CD11. Compared with the Control group, the Stretch group expressed a higher level of SRY-related high mobility group-box 9 (Sox9), proteoglycan 4 (Prg4), and typeⅠcollagen (Col1) genes, while a lower level of matrix metalloproteinase 13 (MMP13) gene . In vivo, mechanical stretch could increase the superficial layer thickness in the rat condylar cartilage compared with the Control group. [Conclusion:] Mechanical stretch can promote the chondrogenic differentiation of rat condylar FCSCs and the cartilage matrix formation in the superficial layer of the condylar cartilage. |
| format | Article |
| id | doaj-art-2b5982d826c54f75b804e9c9f02da7de |
| institution | Kabale University |
| issn | 1005-4979 |
| language | zho |
| publishDate | 2025-02-01 |
| publisher | Editorial Office of Journal of Oral and Maxillofacial Surgery |
| record_format | Article |
| series | Kouqiang hemian waike zazhi |
| spelling | doaj-art-2b5982d826c54f75b804e9c9f02da7de2025-08-25T06:12:00ZzhoEditorial Office of Journal of Oral and Maxillofacial SurgeryKouqiang hemian waike zazhi1005-49792025-02-01351142010.12439/kqhm.1005-4979.2025.01.003Effects of stretch on chondrogenic differentiation of rat condylar fibrocartilage stem cellsGONG Wuyi0MI Xiaohui1LI Yongming2Shanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Orthodontics, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Orthodontics, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Orthodontics, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, China[Objective:] To investigate the effect of mechanical stretch on the chondrogenic differentiation of rat condylar fibrocartilage stem cells (FCSCs). [Methods:] Triple-lineage induction was used to verify the multi-lineage differentiation ability of rat condylar FCSCs, and flow cytometry was conducted to examine the surface markers of rat condylar FCSCs. Afterward, the rat condylar FCSCs were stretched with a strength of 10% for 48 h, and the expression of related chondrogenic genes was detected by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting. We also established rat mandibular advancement (MA) model to find out the in vivo effect of mechanical stretch on the superficial layer of the condylar cartilage. [Results:] The rat condylar FCSCs could differentiate towards adipogenesis, osteogenesis, and chondrogenesis, and they expressed mesenchymal markers CD90 and CD29, while negative for hemopoietic markers CD45 and CD11. Compared with the Control group, the Stretch group expressed a higher level of SRY-related high mobility group-box 9 (Sox9), proteoglycan 4 (Prg4), and typeⅠcollagen (Col1) genes, while a lower level of matrix metalloproteinase 13 (MMP13) gene . In vivo, mechanical stretch could increase the superficial layer thickness in the rat condylar cartilage compared with the Control group. [Conclusion:] Mechanical stretch can promote the chondrogenic differentiation of rat condylar FCSCs and the cartilage matrix formation in the superficial layer of the condylar cartilage.https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2025.01.003mechanical stretchcondylefibrocartilage stem cellschondrogenic differentiation |
| spellingShingle | GONG Wuyi MI Xiaohui LI Yongming Effects of stretch on chondrogenic differentiation of rat condylar fibrocartilage stem cells Kouqiang hemian waike zazhi mechanical stretch condyle fibrocartilage stem cells chondrogenic differentiation |
| title | Effects of stretch on chondrogenic differentiation of rat condylar fibrocartilage stem cells |
| title_full | Effects of stretch on chondrogenic differentiation of rat condylar fibrocartilage stem cells |
| title_fullStr | Effects of stretch on chondrogenic differentiation of rat condylar fibrocartilage stem cells |
| title_full_unstemmed | Effects of stretch on chondrogenic differentiation of rat condylar fibrocartilage stem cells |
| title_short | Effects of stretch on chondrogenic differentiation of rat condylar fibrocartilage stem cells |
| title_sort | effects of stretch on chondrogenic differentiation of rat condylar fibrocartilage stem cells |
| topic | mechanical stretch condyle fibrocartilage stem cells chondrogenic differentiation |
| url | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2025.01.003 |
| work_keys_str_mv | AT gongwuyi effectsofstretchonchondrogenicdifferentiationofratcondylarfibrocartilagestemcells AT mixiaohui effectsofstretchonchondrogenicdifferentiationofratcondylarfibrocartilagestemcells AT liyongming effectsofstretchonchondrogenicdifferentiationofratcondylarfibrocartilagestemcells |