Effects of stretch on chondrogenic differentiation of rat condylar fibrocartilage stem cells
[Objective:] To investigate the effect of mechanical stretch on the chondrogenic differentiation of rat condylar fibrocartilage stem cells (FCSCs). [Methods:] Triple-lineage induction was used to verify the multi-lineage differentiation ability of rat condylar FCSCs, and flow cytometry was conducted...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Oral and Maxillofacial Surgery
2025-02-01
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| Series: | Kouqiang hemian waike zazhi |
| Subjects: | |
| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2025.01.003 |
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| Summary: | [Objective:] To investigate the effect of mechanical stretch on the chondrogenic differentiation of rat condylar fibrocartilage stem cells (FCSCs). [Methods:] Triple-lineage induction was used to verify the multi-lineage differentiation ability of rat condylar FCSCs, and flow cytometry was conducted to examine the surface markers of rat condylar FCSCs. Afterward, the rat condylar FCSCs were stretched with a strength of 10% for 48 h, and the expression of related chondrogenic genes was detected by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting. We also established rat mandibular advancement (MA) model to find out the in vivo effect of mechanical stretch on the superficial layer of the condylar cartilage. [Results:] The rat condylar FCSCs could differentiate towards adipogenesis, osteogenesis, and chondrogenesis, and they expressed mesenchymal markers CD90 and CD29, while negative for hemopoietic markers CD45 and CD11. Compared with the Control group, the Stretch group expressed a higher level of SRY-related high mobility group-box 9 (Sox9), proteoglycan 4 (Prg4), and typeⅠcollagen (Col1) genes, while a lower level of matrix metalloproteinase 13 (MMP13) gene . In vivo, mechanical stretch could increase the superficial layer thickness in the rat condylar cartilage compared with the Control group. [Conclusion:] Mechanical stretch can promote the chondrogenic differentiation of rat condylar FCSCs and the cartilage matrix formation in the superficial layer of the condylar cartilage. |
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| ISSN: | 1005-4979 |