Metagenomic insights into the variation of bacterial communities and potential pathogenic bacteria in drinking water treatment and distribution systems

High-throughput sequencing of 16S rRNA gene amplicons was conducted to characterize the changing patterns of bacterial community and potential pathogens in full-scale drinking water treatment and distribution systems. Results showed that Actinobacteria was the predominant phylum in source water, whi...

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Main Authors: Jiang Ruiming, Li Zihan, Li Qisheng, Liu Yunqi, Zhu Yuning, Chen Zimo, Liu Peng, Jia Shuyu, Ren Hongqiang, Zhang Xu-Xiang
Format: Article
Language:English
Published: Science Press 2022-06-01
Series:National Science Open
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Online Access:https://www.sciengine.com/doi/10.1360/nso/20220015
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author Jiang Ruiming
Li Zihan
Li Qisheng
Liu Yunqi
Zhu Yuning
Chen Zimo
Liu Peng
Jia Shuyu
Ren Hongqiang
Zhang Xu-Xiang
author_facet Jiang Ruiming
Li Zihan
Li Qisheng
Liu Yunqi
Zhu Yuning
Chen Zimo
Liu Peng
Jia Shuyu
Ren Hongqiang
Zhang Xu-Xiang
author_sort Jiang Ruiming
collection DOAJ
description High-throughput sequencing of 16S rRNA gene amplicons was conducted to characterize the changing patterns of bacterial community and potential pathogens in full-scale drinking water treatment and distribution systems. Results showed that Actinobacteria was the predominant phylum in source water, while Proteobacteria dominated after chlorine disinfection and its relative abundance increased from 40.88%±9.45% to 67.86%±27.10%. The genera Pseudarthrobacter, Arenimonas, and Limnohabitans were effectively removed by chlorination, while Phreatobacter, Undibacterium, Pseudomonas, and Sphingomonas within the Proteobacteria phylum were greatly enriched after chlorination. Metagenomic analyses revealed the occurrence of 56 species of potential pathogenic bacteria within 17 genera in drinking water, mainly including Pseudomonas fluorescens and five mycobacteria species, which were also persistent in tap water samples. The bacteria were found to be involved in various pathways, among which considerable groups were related to human diseases, including infectious diseases and even cancers.
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institution DOAJ
issn 2097-1168
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publisher Science Press
record_format Article
series National Science Open
spelling doaj-art-2b473d4dd03e4891ae04fce47f466b122025-08-20T03:15:27ZengScience PressNational Science Open2097-11682022-06-01110.1360/nso/20220015eb33e642Metagenomic insights into the variation of bacterial communities and potential pathogenic bacteria in drinking water treatment and distribution systemsJiang Ruiming0Li Zihan1Li Qisheng2Liu Yunqi3Zhu Yuning4Chen Zimo5Liu Peng6Jia Shuyu7Ren Hongqiang8Zhang Xu-Xiang9["State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China"]["State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China"]["State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China","China Three Gorges Construction Engineering Corporation, Beijing 100048, China"]["State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China","Nanjing Foreign Language School, Nanjing 210008, China"]["State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China","Nanjing Foreign Language School, Nanjing 210008, China"]["State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China","Nanjing Foreign Language School, Nanjing 210008, China"]["State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China"]["State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China"]["State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China"]["State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China"]High-throughput sequencing of 16S rRNA gene amplicons was conducted to characterize the changing patterns of bacterial community and potential pathogens in full-scale drinking water treatment and distribution systems. Results showed that Actinobacteria was the predominant phylum in source water, while Proteobacteria dominated after chlorine disinfection and its relative abundance increased from 40.88%±9.45% to 67.86%±27.10%. The genera Pseudarthrobacter, Arenimonas, and Limnohabitans were effectively removed by chlorination, while Phreatobacter, Undibacterium, Pseudomonas, and Sphingomonas within the Proteobacteria phylum were greatly enriched after chlorination. Metagenomic analyses revealed the occurrence of 56 species of potential pathogenic bacteria within 17 genera in drinking water, mainly including Pseudomonas fluorescens and five mycobacteria species, which were also persistent in tap water samples. The bacteria were found to be involved in various pathways, among which considerable groups were related to human diseases, including infectious diseases and even cancers.https://www.sciengine.com/doi/10.1360/nso/20220015drinking waterchlorinationbacterial communitypotential pathogenic bacteriahigh-throughput sequencingmetagenomics
spellingShingle Jiang Ruiming
Li Zihan
Li Qisheng
Liu Yunqi
Zhu Yuning
Chen Zimo
Liu Peng
Jia Shuyu
Ren Hongqiang
Zhang Xu-Xiang
Metagenomic insights into the variation of bacterial communities and potential pathogenic bacteria in drinking water treatment and distribution systems
National Science Open
drinking water
chlorination
bacterial community
potential pathogenic bacteria
high-throughput sequencing
metagenomics
title Metagenomic insights into the variation of bacterial communities and potential pathogenic bacteria in drinking water treatment and distribution systems
title_full Metagenomic insights into the variation of bacterial communities and potential pathogenic bacteria in drinking water treatment and distribution systems
title_fullStr Metagenomic insights into the variation of bacterial communities and potential pathogenic bacteria in drinking water treatment and distribution systems
title_full_unstemmed Metagenomic insights into the variation of bacterial communities and potential pathogenic bacteria in drinking water treatment and distribution systems
title_short Metagenomic insights into the variation of bacterial communities and potential pathogenic bacteria in drinking water treatment and distribution systems
title_sort metagenomic insights into the variation of bacterial communities and potential pathogenic bacteria in drinking water treatment and distribution systems
topic drinking water
chlorination
bacterial community
potential pathogenic bacteria
high-throughput sequencing
metagenomics
url https://www.sciengine.com/doi/10.1360/nso/20220015
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