Simultaneous Determination of Hormonal Residues in Treated Waters Using Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry

In the last years, hormone consumption has increased exponentially. Because of that, hormone compounds are considered emerging pollutants since several studies have determinted their presence in water influents and effluents of wastewater treatment plants (WWTPs). In this study, a quantitative metho...

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Main Authors: Rayco Guedes-Alonso, Zoraida Sosa-Ferrera, José Juan Santana-Rodríguez
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/2013/210653
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author Rayco Guedes-Alonso
Zoraida Sosa-Ferrera
José Juan Santana-Rodríguez
author_facet Rayco Guedes-Alonso
Zoraida Sosa-Ferrera
José Juan Santana-Rodríguez
author_sort Rayco Guedes-Alonso
collection DOAJ
description In the last years, hormone consumption has increased exponentially. Because of that, hormone compounds are considered emerging pollutants since several studies have determinted their presence in water influents and effluents of wastewater treatment plants (WWTPs). In this study, a quantitative method for the simultaneous determination of oestrogens (estrone, 17β-estradiol, estriol, 17α-ethinylestradiol, and diethylstilbestrol), androgens (testosterone), and progestogens (norgestrel and megestrol acetate) has been developed to determine these compounds in wastewater samples. Due to the very low concentrations of target compounds in the environment, a solid phase extraction procedure has been optimized and developed to extract and preconcentrate the analytes. Determination and quantification were performed by ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The method developed presents satisfactory limits of detection (between 0.15 and 9.35 ng·L−1), good recoveries (between 73 and 90% for the most of compounds), and low relative standard deviations (under 8.4%). Samples from influents and effluents of two wastewater treatment plants of Gran Canaria (Spain) were analyzed using the proposed method, finding several hormones with concentrations ranged from 5 to 300 ng·L−1.
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series Journal of Analytical Methods in Chemistry
spelling doaj-art-2b3e1749f82a47a6b945df98e259bc372025-08-20T02:04:10ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/210653210653Simultaneous Determination of Hormonal Residues in Treated Waters Using Ultrahigh Performance Liquid Chromatography-Tandem Mass SpectrometryRayco Guedes-Alonso0Zoraida Sosa-Ferrera1José Juan Santana-Rodríguez2Departamento de Química, Universidad de Las Palmas de Gran Canaria, 35017 Las Palmas de Gran Canaria, SpainDepartamento de Química, Universidad de Las Palmas de Gran Canaria, 35017 Las Palmas de Gran Canaria, SpainDepartamento de Química, Universidad de Las Palmas de Gran Canaria, 35017 Las Palmas de Gran Canaria, SpainIn the last years, hormone consumption has increased exponentially. Because of that, hormone compounds are considered emerging pollutants since several studies have determinted their presence in water influents and effluents of wastewater treatment plants (WWTPs). In this study, a quantitative method for the simultaneous determination of oestrogens (estrone, 17β-estradiol, estriol, 17α-ethinylestradiol, and diethylstilbestrol), androgens (testosterone), and progestogens (norgestrel and megestrol acetate) has been developed to determine these compounds in wastewater samples. Due to the very low concentrations of target compounds in the environment, a solid phase extraction procedure has been optimized and developed to extract and preconcentrate the analytes. Determination and quantification were performed by ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The method developed presents satisfactory limits of detection (between 0.15 and 9.35 ng·L−1), good recoveries (between 73 and 90% for the most of compounds), and low relative standard deviations (under 8.4%). Samples from influents and effluents of two wastewater treatment plants of Gran Canaria (Spain) were analyzed using the proposed method, finding several hormones with concentrations ranged from 5 to 300 ng·L−1.http://dx.doi.org/10.1155/2013/210653
spellingShingle Rayco Guedes-Alonso
Zoraida Sosa-Ferrera
José Juan Santana-Rodríguez
Simultaneous Determination of Hormonal Residues in Treated Waters Using Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry
Journal of Analytical Methods in Chemistry
title Simultaneous Determination of Hormonal Residues in Treated Waters Using Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry
title_full Simultaneous Determination of Hormonal Residues in Treated Waters Using Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry
title_fullStr Simultaneous Determination of Hormonal Residues in Treated Waters Using Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry
title_full_unstemmed Simultaneous Determination of Hormonal Residues in Treated Waters Using Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry
title_short Simultaneous Determination of Hormonal Residues in Treated Waters Using Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry
title_sort simultaneous determination of hormonal residues in treated waters using ultrahigh performance liquid chromatography tandem mass spectrometry
url http://dx.doi.org/10.1155/2013/210653
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AT zoraidasosaferrera simultaneousdeterminationofhormonalresiduesintreatedwatersusingultrahighperformanceliquidchromatographytandemmassspectrometry
AT josejuansantanarodriguez simultaneousdeterminationofhormonalresiduesintreatedwatersusingultrahighperformanceliquidchromatographytandemmassspectrometry