Determination of Ruboxistaurin analysis in rat plasma utilizing LC–MS/MS technique
Background: Ruboxistaurin (RBX) used to treat retinopathy in diabetic patients which caused by microvascular damage and leakage which contributes to visual loss. There are no published studies on the use of liquid chromatography-tandem mass spectrometry for development and validation of a simple, se...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2023-04-01
|
| Series: | Saudi Pharmaceutical Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016423000373 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849307526541082624 |
|---|---|
| author | Bushra T. AlQuadeib Fadilah S. Aleanizy Fulwah Y. Alqahtani Rehab A. Alshammari Amal Aldarwesh Ibrahim Alsarra |
| author_facet | Bushra T. AlQuadeib Fadilah S. Aleanizy Fulwah Y. Alqahtani Rehab A. Alshammari Amal Aldarwesh Ibrahim Alsarra |
| author_sort | Bushra T. AlQuadeib |
| collection | DOAJ |
| description | Background: Ruboxistaurin (RBX) used to treat retinopathy in diabetic patients which caused by microvascular damage and leakage which contributes to visual loss. There are no published studies on the use of liquid chromatography-tandem mass spectrometry for development and validation of a simple, sensitive, and accurate method for measuring RBX in rat plasma. Method: Chromatographic separation of RBX was achieved using ultra-performance liquid chromatography. Multiple-reaction monitoring quantification used RBX [M + H] + ion at m/z 469.18 and daughter ions at m/z 84, 58.12, and 98.10. Atorvastatin was used as internal standard (IS), has a single daughter ion, and was identified using m/z 559.6 → 249.9. Validation of the liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for RBX in rat plasma for linearity (greater than0.997) was carried out at 25–1000 ng/mL. Results: In rat plasma, the accuracy was within 3.4%, and the intra- and inter-day precision was within 11.8%. Stability, recovery, and matrix effect were all within acceptable limits. The drug retention time (0.85 ± 0.03 min) was remarkably short. Conclusion: The method developed in the current study is suitable to quantify RBX in plasma or bulk doses. |
| format | Article |
| id | doaj-art-2b2712f1e9d74fb88e4cdeaffb789388 |
| institution | Kabale University |
| issn | 1319-0164 |
| language | English |
| publishDate | 2023-04-01 |
| publisher | Springer |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj-art-2b2712f1e9d74fb88e4cdeaffb7893882025-08-20T03:54:43ZengSpringerSaudi Pharmaceutical Journal1319-01642023-04-0131454755310.1016/j.jsps.2023.02.007Determination of Ruboxistaurin analysis in rat plasma utilizing LC–MS/MS techniqueBushra T. AlQuadeib0Fadilah S. Aleanizy1Fulwah Y. Alqahtani2Rehab A. Alshammari3Amal Aldarwesh4Ibrahim Alsarra5Department of Pharmaceutics, College of Pharmacy, King Saud University, Saudi Arabia, P.O. Box 84428, Riyadh 11671, Saudi Arabia; Corresponding author.Department of Pharmaceutics, College of Pharmacy, King Saud University, Saudi Arabia, P.O. Box 84428, Riyadh 11671, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Saudi Arabia, P.O. Box 84428, Riyadh 11671, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Saudi Arabia, P.O. Box 84428, Riyadh 11671, Saudi ArabiaDepartment of Optometry, College of Applied Medical Sciences, King Saud University, P.O. 11671, Riyadh 11671, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Saudi Arabia, P.O. Box 84428, Riyadh 11671, Saudi ArabiaBackground: Ruboxistaurin (RBX) used to treat retinopathy in diabetic patients which caused by microvascular damage and leakage which contributes to visual loss. There are no published studies on the use of liquid chromatography-tandem mass spectrometry for development and validation of a simple, sensitive, and accurate method for measuring RBX in rat plasma. Method: Chromatographic separation of RBX was achieved using ultra-performance liquid chromatography. Multiple-reaction monitoring quantification used RBX [M + H] + ion at m/z 469.18 and daughter ions at m/z 84, 58.12, and 98.10. Atorvastatin was used as internal standard (IS), has a single daughter ion, and was identified using m/z 559.6 → 249.9. Validation of the liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for RBX in rat plasma for linearity (greater than0.997) was carried out at 25–1000 ng/mL. Results: In rat plasma, the accuracy was within 3.4%, and the intra- and inter-day precision was within 11.8%. Stability, recovery, and matrix effect were all within acceptable limits. The drug retention time (0.85 ± 0.03 min) was remarkably short. Conclusion: The method developed in the current study is suitable to quantify RBX in plasma or bulk doses.http://www.sciencedirect.com/science/article/pii/S1319016423000373RuboxistaurinValidationLC-MS/MSPlasmaRat |
| spellingShingle | Bushra T. AlQuadeib Fadilah S. Aleanizy Fulwah Y. Alqahtani Rehab A. Alshammari Amal Aldarwesh Ibrahim Alsarra Determination of Ruboxistaurin analysis in rat plasma utilizing LC–MS/MS technique Saudi Pharmaceutical Journal Ruboxistaurin Validation LC-MS/MS Plasma Rat |
| title | Determination of Ruboxistaurin analysis in rat plasma utilizing LC–MS/MS technique |
| title_full | Determination of Ruboxistaurin analysis in rat plasma utilizing LC–MS/MS technique |
| title_fullStr | Determination of Ruboxistaurin analysis in rat plasma utilizing LC–MS/MS technique |
| title_full_unstemmed | Determination of Ruboxistaurin analysis in rat plasma utilizing LC–MS/MS technique |
| title_short | Determination of Ruboxistaurin analysis in rat plasma utilizing LC–MS/MS technique |
| title_sort | determination of ruboxistaurin analysis in rat plasma utilizing lc ms ms technique |
| topic | Ruboxistaurin Validation LC-MS/MS Plasma Rat |
| url | http://www.sciencedirect.com/science/article/pii/S1319016423000373 |
| work_keys_str_mv | AT bushratalquadeib determinationofruboxistaurinanalysisinratplasmautilizinglcmsmstechnique AT fadilahsaleanizy determinationofruboxistaurinanalysisinratplasmautilizinglcmsmstechnique AT fulwahyalqahtani determinationofruboxistaurinanalysisinratplasmautilizinglcmsmstechnique AT rehabaalshammari determinationofruboxistaurinanalysisinratplasmautilizinglcmsmstechnique AT amalaldarwesh determinationofruboxistaurinanalysisinratplasmautilizinglcmsmstechnique AT ibrahimalsarra determinationofruboxistaurinanalysisinratplasmautilizinglcmsmstechnique |