Genomic plasticity of extensively drug-resistant and multidrug-resistant Acinetobacter baumannii ST208 isolates from a fatal outbreak
Background: The prevalence of multidrug-resistant Acinetobacter baumannii (MDRA) has rapidly increased and is linked to severe nosocomial infections. MDRA outbreaks in a Japanese hospital were analysed using whole-genome sequencing. Methods: Antibiotic susceptibility testing was performed on clinica...
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Elsevier
2025-05-01
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| author | Satoshi Nishida Yasuo Ono |
| author_facet | Satoshi Nishida Yasuo Ono |
| author_sort | Satoshi Nishida |
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| description | Background: The prevalence of multidrug-resistant Acinetobacter baumannii (MDRA) has rapidly increased and is linked to severe nosocomial infections. MDRA outbreaks in a Japanese hospital were analysed using whole-genome sequencing. Methods: Antibiotic susceptibility testing was performed on clinical isolates from hospitalised patients before and during the 2009 and 2010 outbreaks. Whole-genome sequencing was conducted to identify acquired antibiotic-resistance genes and genetic mutations. Results: Clinical A. baumannii isolates were resistant to β-lactams (broad-spectrum cephalosporins and carbapenems), aminoglycosides, chloramphenicol, fosfomycin, fluoroquinolones, tetracyclines, and trimethoprim-sulfamethoxazole. MDRA isolates harboured aac(6′)-Ib-cr, abaF, armA, blaADC-30, blaTEM-1, and blaOXA-82, or both blaOXA-66 and blaOXA-23, catB8, mphE, msrE, and tet(B). blaOXA-82 genes were recombinationally multiplied. Quinolone resistance was also associated with gyrA S81L and parC S84L mutations. The MDRA isolates belonged to Oxford sequence type (ST) 208 and Pasteur ST2. Three of the 15 isolates developed an extensively drug-resistant (XDR) phenotype, and two isolates harboured an adeS mutation. Conclusions: We identified molecular resistance markers in three XDR and one MDR isolate and provided a genomic description of resistance and virulence, as well as the origins of the isolates. The isolates are closely related to MDRA Oxford ST208 and Pasteur ST2, identified in Asia and Australia. MDRA isolates are of concern in both hospital and community settings in the Western Pacific region. |
| format | Article |
| id | doaj-art-2b237d84de494972897a0e26ce26f8df |
| institution | OA Journals |
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| spelling | doaj-art-2b237d84de494972897a0e26ce26f8df2025-08-20T02:15:51ZengElsevierJournal of Infection and Public Health1876-03412025-05-0118510273910.1016/j.jiph.2025.102739Genomic plasticity of extensively drug-resistant and multidrug-resistant Acinetobacter baumannii ST208 isolates from a fatal outbreakSatoshi Nishida0Yasuo Ono1Department of Microbiology and Immunology, Teikyo University School of Medicine, Kaga, Itabashi, Tokyo, Japan; Correspondence to: Department of Microbiology and Immunology, Teikyo University School of Medicine, Itabashi, Tokyo 173-8605, JapanDepartment of Microbiology and Immunology, Teikyo University School of Medicine, Kaga, Itabashi, Tokyo, Japan; Faculty of Health and Medical Science, Teikyo Heisei University, Higashi-Ikebukuro, Toshima, Tokyo, JapanBackground: The prevalence of multidrug-resistant Acinetobacter baumannii (MDRA) has rapidly increased and is linked to severe nosocomial infections. MDRA outbreaks in a Japanese hospital were analysed using whole-genome sequencing. Methods: Antibiotic susceptibility testing was performed on clinical isolates from hospitalised patients before and during the 2009 and 2010 outbreaks. Whole-genome sequencing was conducted to identify acquired antibiotic-resistance genes and genetic mutations. Results: Clinical A. baumannii isolates were resistant to β-lactams (broad-spectrum cephalosporins and carbapenems), aminoglycosides, chloramphenicol, fosfomycin, fluoroquinolones, tetracyclines, and trimethoprim-sulfamethoxazole. MDRA isolates harboured aac(6′)-Ib-cr, abaF, armA, blaADC-30, blaTEM-1, and blaOXA-82, or both blaOXA-66 and blaOXA-23, catB8, mphE, msrE, and tet(B). blaOXA-82 genes were recombinationally multiplied. Quinolone resistance was also associated with gyrA S81L and parC S84L mutations. The MDRA isolates belonged to Oxford sequence type (ST) 208 and Pasteur ST2. Three of the 15 isolates developed an extensively drug-resistant (XDR) phenotype, and two isolates harboured an adeS mutation. Conclusions: We identified molecular resistance markers in three XDR and one MDR isolate and provided a genomic description of resistance and virulence, as well as the origins of the isolates. The isolates are closely related to MDRA Oxford ST208 and Pasteur ST2, identified in Asia and Australia. MDRA isolates are of concern in both hospital and community settings in the Western Pacific region.http://www.sciencedirect.com/science/article/pii/S1876034125000887Acinetobacter baumanniiAminoglycosidesarmAExtensively drug-resistantGenomicsCarbapenems |
| spellingShingle | Satoshi Nishida Yasuo Ono Genomic plasticity of extensively drug-resistant and multidrug-resistant Acinetobacter baumannii ST208 isolates from a fatal outbreak Journal of Infection and Public Health Acinetobacter baumannii Aminoglycosides armA Extensively drug-resistant Genomics Carbapenems |
| title | Genomic plasticity of extensively drug-resistant and multidrug-resistant Acinetobacter baumannii ST208 isolates from a fatal outbreak |
| title_full | Genomic plasticity of extensively drug-resistant and multidrug-resistant Acinetobacter baumannii ST208 isolates from a fatal outbreak |
| title_fullStr | Genomic plasticity of extensively drug-resistant and multidrug-resistant Acinetobacter baumannii ST208 isolates from a fatal outbreak |
| title_full_unstemmed | Genomic plasticity of extensively drug-resistant and multidrug-resistant Acinetobacter baumannii ST208 isolates from a fatal outbreak |
| title_short | Genomic plasticity of extensively drug-resistant and multidrug-resistant Acinetobacter baumannii ST208 isolates from a fatal outbreak |
| title_sort | genomic plasticity of extensively drug resistant and multidrug resistant acinetobacter baumannii st208 isolates from a fatal outbreak |
| topic | Acinetobacter baumannii Aminoglycosides armA Extensively drug-resistant Genomics Carbapenems |
| url | http://www.sciencedirect.com/science/article/pii/S1876034125000887 |
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