Computer-Assisted Reconstruction and Motion Analysis of the Three-Dimensional Cell
Even though several microscopic techniques provide three-dimensional (3D) information on fixed and living cells, the perception persists that cells are two-dimensional (2D). Cells are, in fact, 3D and their behavior, including the extension of pseudopods, includes an important 3D component. Although...
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| Format: | Article |
| Language: | English |
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Wiley
2003-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2003.70 |
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| _version_ | 1850229531138326528 |
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| author | David R. Soll Deborah Wessels Paul J. Heid Edward Voss |
| author_facet | David R. Soll Deborah Wessels Paul J. Heid Edward Voss |
| author_sort | David R. Soll |
| collection | DOAJ |
| description | Even though several microscopic techniques provide three-dimensional (3D) information on fixed and living cells, the perception persists that cells are two-dimensional (2D). Cells are, in fact, 3D and their behavior, including the extension of pseudopods, includes an important 3D component. Although treating the cell as a 2D entity has proven effective in understanding how cells locomote, and in identifying defects in a variety of mutant and abnormal cells, there are cases in which 3D reconstruction and analysis are essential. Here, we describe advanced computer-assisted 3D reconstruction and motion analysis programs for both individual live, crawling cells and developing embryos. These systems (3D-DIAS, 3D-DIASemb) can be used to reconstruct and motion analyze at short time intervals the nucleus and pseudopodia as well as the entire surface of a single migrating cell, or every cell and nucleus in a developing embryo. Because all images are converted to mathematical representations, a variety of motility and dynamic morphology parameters can be computed that have proven quite valuable in the identification of mutant behaviors. We also describe examples of mutant behaviors in Dictyostelium that were revealed through 3D analysis. |
| format | Article |
| id | doaj-art-2b1605f307b2411c8d26ef635f8d1943 |
| institution | OA Journals |
| issn | 1537-744X |
| language | English |
| publishDate | 2003-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-2b1605f307b2411c8d26ef635f8d19432025-08-20T02:04:11ZengWileyThe Scientific World Journal1537-744X2003-01-01382784110.1100/tsw.2003.70Computer-Assisted Reconstruction and Motion Analysis of the Three-Dimensional CellDavid R. Soll0Deborah Wessels1Paul J. Heid2Edward Voss3Department of Biological Sciences, The University of Iowa, Iowa City, Iowa 52242, USADepartment of Biological Sciences, The University of Iowa, Iowa City, Iowa 52242, USADepartment of Biological Sciences, The University of Iowa, Iowa City, Iowa 52242, USADepartment of Biological Sciences, The University of Iowa, Iowa City, Iowa 52242, USAEven though several microscopic techniques provide three-dimensional (3D) information on fixed and living cells, the perception persists that cells are two-dimensional (2D). Cells are, in fact, 3D and their behavior, including the extension of pseudopods, includes an important 3D component. Although treating the cell as a 2D entity has proven effective in understanding how cells locomote, and in identifying defects in a variety of mutant and abnormal cells, there are cases in which 3D reconstruction and analysis are essential. Here, we describe advanced computer-assisted 3D reconstruction and motion analysis programs for both individual live, crawling cells and developing embryos. These systems (3D-DIAS, 3D-DIASemb) can be used to reconstruct and motion analyze at short time intervals the nucleus and pseudopodia as well as the entire surface of a single migrating cell, or every cell and nucleus in a developing embryo. Because all images are converted to mathematical representations, a variety of motility and dynamic morphology parameters can be computed that have proven quite valuable in the identification of mutant behaviors. We also describe examples of mutant behaviors in Dictyostelium that were revealed through 3D analysis.http://dx.doi.org/10.1100/tsw.2003.70 |
| spellingShingle | David R. Soll Deborah Wessels Paul J. Heid Edward Voss Computer-Assisted Reconstruction and Motion Analysis of the Three-Dimensional Cell The Scientific World Journal |
| title | Computer-Assisted Reconstruction and Motion Analysis of the Three-Dimensional Cell |
| title_full | Computer-Assisted Reconstruction and Motion Analysis of the Three-Dimensional Cell |
| title_fullStr | Computer-Assisted Reconstruction and Motion Analysis of the Three-Dimensional Cell |
| title_full_unstemmed | Computer-Assisted Reconstruction and Motion Analysis of the Three-Dimensional Cell |
| title_short | Computer-Assisted Reconstruction and Motion Analysis of the Three-Dimensional Cell |
| title_sort | computer assisted reconstruction and motion analysis of the three dimensional cell |
| url | http://dx.doi.org/10.1100/tsw.2003.70 |
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