Evaluating protein complexes between human aquaporin and calmodulin using biomolecular fluorescence complementation
Abstract Aquaporins (AQPs) are a family of integral membrane proteins crucial for the flow of water and other small molecules across cellular membranes. The involvement of calmodulin (CaM), a multifunctional calcium-binding protein, has emerged as a central regulator for specific aquaporin homologue...
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Nature Portfolio
2025-08-01
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| Online Access: | https://doi.org/10.1038/s41598-025-12865-z |
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| author | Jessica Glas Johanna Landén Kristina Hedfalk |
| author_facet | Jessica Glas Johanna Landén Kristina Hedfalk |
| author_sort | Jessica Glas |
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| description | Abstract Aquaporins (AQPs) are a family of integral membrane proteins crucial for the flow of water and other small molecules across cellular membranes. The involvement of calmodulin (CaM), a multifunctional calcium-binding protein, has emerged as a central regulator for specific aquaporin homologues from eukaryotes. Using a systematic approach, applying advanced high throughput screening methods in vivo, combining flow cytometry with microscopy, we have evaluated the putative interaction between CaM and the 13 human AQP homologues recombinantly produced in the yeast Saccharomyces cerevisiae. This comprehensive approach is complemented by a theoretical validation of potential CaM binding sites and a review of confirmed CaM binding locations from previous research. Our investigation is based on the established interaction of hAQP0 and CaM and we have successfully validated the binding of hAQP1 and hAQP4 to CaM. Noteworthy, discernibly high fluorescence frequency signals were observed for hAQP8 and hAQP9, which did not correlate with a particularly high production level, supporting protein complex formation with CaM for those AQP homologues. Overall, we present a systematic approach to screen novel membrane protein interactions in vivo, relying on co-expression in yeast of Bimolecular Fluorescence Complementation (BiFC) complexes providing new insights into the regulation of the hAQPs. |
| format | Article |
| id | doaj-art-2afea6c8bc504ec187358690d8051692 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-2afea6c8bc504ec187358690d80516922025-08-20T03:42:33ZengNature PortfolioScientific Reports2045-23222025-08-0115111010.1038/s41598-025-12865-zEvaluating protein complexes between human aquaporin and calmodulin using biomolecular fluorescence complementationJessica Glas0Johanna Landén1Kristina Hedfalk2Department of Chemistry and Molecular Biology, Gothenburg UniversityDepartment of Chemistry and Molecular Biology, Gothenburg UniversityDepartment of Chemistry and Molecular Biology, Gothenburg UniversityAbstract Aquaporins (AQPs) are a family of integral membrane proteins crucial for the flow of water and other small molecules across cellular membranes. The involvement of calmodulin (CaM), a multifunctional calcium-binding protein, has emerged as a central regulator for specific aquaporin homologues from eukaryotes. Using a systematic approach, applying advanced high throughput screening methods in vivo, combining flow cytometry with microscopy, we have evaluated the putative interaction between CaM and the 13 human AQP homologues recombinantly produced in the yeast Saccharomyces cerevisiae. This comprehensive approach is complemented by a theoretical validation of potential CaM binding sites and a review of confirmed CaM binding locations from previous research. Our investigation is based on the established interaction of hAQP0 and CaM and we have successfully validated the binding of hAQP1 and hAQP4 to CaM. Noteworthy, discernibly high fluorescence frequency signals were observed for hAQP8 and hAQP9, which did not correlate with a particularly high production level, supporting protein complex formation with CaM for those AQP homologues. Overall, we present a systematic approach to screen novel membrane protein interactions in vivo, relying on co-expression in yeast of Bimolecular Fluorescence Complementation (BiFC) complexes providing new insights into the regulation of the hAQPs.https://doi.org/10.1038/s41598-025-12865-zAquaporinCalmodulinSaccharomyces cerevisiaeBimolecular fluorescence complementation |
| spellingShingle | Jessica Glas Johanna Landén Kristina Hedfalk Evaluating protein complexes between human aquaporin and calmodulin using biomolecular fluorescence complementation Scientific Reports Aquaporin Calmodulin Saccharomyces cerevisiae Bimolecular fluorescence complementation |
| title | Evaluating protein complexes between human aquaporin and calmodulin using biomolecular fluorescence complementation |
| title_full | Evaluating protein complexes between human aquaporin and calmodulin using biomolecular fluorescence complementation |
| title_fullStr | Evaluating protein complexes between human aquaporin and calmodulin using biomolecular fluorescence complementation |
| title_full_unstemmed | Evaluating protein complexes between human aquaporin and calmodulin using biomolecular fluorescence complementation |
| title_short | Evaluating protein complexes between human aquaporin and calmodulin using biomolecular fluorescence complementation |
| title_sort | evaluating protein complexes between human aquaporin and calmodulin using biomolecular fluorescence complementation |
| topic | Aquaporin Calmodulin Saccharomyces cerevisiae Bimolecular fluorescence complementation |
| url | https://doi.org/10.1038/s41598-025-12865-z |
| work_keys_str_mv | AT jessicaglas evaluatingproteincomplexesbetweenhumanaquaporinandcalmodulinusingbiomolecularfluorescencecomplementation AT johannalanden evaluatingproteincomplexesbetweenhumanaquaporinandcalmodulinusingbiomolecularfluorescencecomplementation AT kristinahedfalk evaluatingproteincomplexesbetweenhumanaquaporinandcalmodulinusingbiomolecularfluorescencecomplementation |