Prednisone inhibits osteosarcoma cell by regulating the Wnt/β-catenin signaling pathway
Prednisone, a synthetic glucocorticoid, possesses a moderate duration of action in the human body. To investigate the effect of prednisone on osteosarcoma cells in vitro, CCK8, wound healing, Transwell assays, flow-cytometry, western blot, and ELISA were used to measure cell proliferation, cell mig...
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| Format: | Article |
| Language: | English |
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Bangladesh Pharmacological Society
2024-12-01
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| Series: | Bangladesh Journal of Pharmacology |
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| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/76379 |
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| author | Yo Zhang Hongmei Zhang Shanshan Yang Xiufeng Gao Wenhao Guo |
| author_facet | Yo Zhang Hongmei Zhang Shanshan Yang Xiufeng Gao Wenhao Guo |
| author_sort | Yo Zhang |
| collection | DOAJ |
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Prednisone, a synthetic glucocorticoid, possesses a moderate duration of action in the human body. To investigate the effect of prednisone on osteosarcoma cells in vitro, CCK8, wound healing, Transwell assays, flow-cytometry, western blot, and ELISA were used to measure cell proliferation, cell migration, cell invasion, cell cycle, cell apoptosis, and the key proteins in Wnt/β-catenin pathway. The results showed prednisone could inhibit the proliferation in osteosarcoma cells and exhibit lower proliferation toxicity towards normal bone cells compared to cisplatin by 17.5% (p<0.001). Cell apoptosis was increased by 6.6 and 7.4%, cell migration was decreased to 62.6 and –23.9%, G0/G1 phase cells were increased by 33.0 and 17.9%, and S phase cells were reduced by 29.6 and 18.0% (all p<0.001) in Mg63 and Saos-2 cells respectively after prednisone treatment. The expressions of β-catenin, cMYC, cyclin D1, and MMP7 were also reduced. In conclusion, prednisone inhibits osteosarcoma cells by regulating the Wnt/β-catenin signaling pathway.
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| format | Article |
| id | doaj-art-2afe32d8d7244ffcb1ec73bea320d06d |
| institution | Kabale University |
| issn | 1991-0088 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj-art-2afe32d8d7244ffcb1ec73bea320d06d2025-02-02T13:09:58ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882024-12-0119310.3329/bjp.v19i3.76379Prednisone inhibits osteosarcoma cell by regulating the Wnt/β-catenin signaling pathwayYo Zhang0https://orcid.org/0009-0007-6550-1358Hongmei Zhang1https://orcid.org/0009-0009-3152-9154Shanshan Yang2https://orcid.org/0009-0005-8318-9666Xiufeng Gao3https://orcid.org/0000-0002-2846-0741Wenhao Guo4https://orcid.org/0000-0002-5342-7695Clinical Translational Innovation Center/Molecular Medicine Research Center, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China.Clinical Translational Innovation Center/Molecular Medicine Research Center, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China.Clinical Translational Innovation Center/Molecular Medicine Research Center, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China.West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan Province, China..Department of Abdominal Oncology, Cancer Center and State Key Laboratory of Biotherapy, Medical School, West China Hospital, Sichuan University, Sichuan Province, China. Prednisone, a synthetic glucocorticoid, possesses a moderate duration of action in the human body. To investigate the effect of prednisone on osteosarcoma cells in vitro, CCK8, wound healing, Transwell assays, flow-cytometry, western blot, and ELISA were used to measure cell proliferation, cell migration, cell invasion, cell cycle, cell apoptosis, and the key proteins in Wnt/β-catenin pathway. The results showed prednisone could inhibit the proliferation in osteosarcoma cells and exhibit lower proliferation toxicity towards normal bone cells compared to cisplatin by 17.5% (p<0.001). Cell apoptosis was increased by 6.6 and 7.4%, cell migration was decreased to 62.6 and –23.9%, G0/G1 phase cells were increased by 33.0 and 17.9%, and S phase cells were reduced by 29.6 and 18.0% (all p<0.001) in Mg63 and Saos-2 cells respectively after prednisone treatment. The expressions of β-catenin, cMYC, cyclin D1, and MMP7 were also reduced. In conclusion, prednisone inhibits osteosarcoma cells by regulating the Wnt/β-catenin signaling pathway. https://www.banglajol.info/index.php/BJP/article/view/76379OsteosarcomaPrednisoneWestern blotWnt/β-catenin signaling |
| spellingShingle | Yo Zhang Hongmei Zhang Shanshan Yang Xiufeng Gao Wenhao Guo Prednisone inhibits osteosarcoma cell by regulating the Wnt/β-catenin signaling pathway Bangladesh Journal of Pharmacology Osteosarcoma Prednisone Western blot Wnt/β-catenin signaling |
| title | Prednisone inhibits osteosarcoma cell by regulating the Wnt/β-catenin signaling pathway |
| title_full | Prednisone inhibits osteosarcoma cell by regulating the Wnt/β-catenin signaling pathway |
| title_fullStr | Prednisone inhibits osteosarcoma cell by regulating the Wnt/β-catenin signaling pathway |
| title_full_unstemmed | Prednisone inhibits osteosarcoma cell by regulating the Wnt/β-catenin signaling pathway |
| title_short | Prednisone inhibits osteosarcoma cell by regulating the Wnt/β-catenin signaling pathway |
| title_sort | prednisone inhibits osteosarcoma cell by regulating the wnt β catenin signaling pathway |
| topic | Osteosarcoma Prednisone Western blot Wnt/β-catenin signaling |
| url | https://www.banglajol.info/index.php/BJP/article/view/76379 |
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