MALDI-TOF MS ANALYSIS OF LONG-TERM STORED STOCK CANDIDA STRAINS IDENTIFIED BY MOLECULAR AND CONVENTIONAL METHODS
Objective: In recent years, the rapid and accurate identification of Candida isolates at species level has been of great importance for reasons such as increased incidence of infections due to Candida species and differing antifungal susceptibilities by species. Herein, we aimed to investigate the e...
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Istanbul University Press
2021-01-01
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| Series: | İstanbul Tıp Fakültesi Dergisi |
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| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/AB86A576A6D54B33BD8B8D6904467B04 |
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| author | Tuğrul Hoşbul Fatih Şahiner Ramazan Gümral Sinem Kaya Bayhan Bektöre Kemal Tekin Şinasi Taner Yıldıran |
| author_facet | Tuğrul Hoşbul Fatih Şahiner Ramazan Gümral Sinem Kaya Bayhan Bektöre Kemal Tekin Şinasi Taner Yıldıran |
| author_sort | Tuğrul Hoşbul |
| collection | DOAJ |
| description | Objective: In recent years, the rapid and accurate identification of Candida isolates at species level has been of great importance for reasons such as increased incidence of infections due to Candida species and differing antifungal susceptibilities by species. Herein, we aimed to investigate the efficacy of the MALDI-TOF MS method in the identification of Candida clinical isolates stored at -20°C for years. Material and Method: Twenty eight Candida strains stored at -20°C for more than 10 years without any recultivation and identified by various methods such as API® ID 32C, CHROMagar Candida and PCR-RFLP were included in the study. Isolates were subcultured and analysed by MALDI-TOF MS. An unidentified isolate at the species level previously and another isolate with low MALDI-TOF MS score were analyzed by ITS sequencing. Additionally, MALDI-TOF MS scores of 57 Candida strains isolated from routine cultures were compared with study isolates. Results: Of them, 11 (39.3%) isolates were identified at the species level and 15 (53.6%) were identified at the genus level, while two isolates were outside the reliable identification limits by MALDI-TOF MS. MALDI-TOF MS yielded an accurate identification at the species level in all 28 isolates identified by various methods even though different scores were obtained. Conclusion: MALDI-TOF MS is a cost effective, easy to use, fast and reliable method compared with the conventional and molecular methods for identification of stock Candida isolates preserved for many years. In conclusion, although a limited number of stock Candida isolates were included in the study, low identification scores for Candida isolates may be valuable in the MALDI-TOF MS analysis. |
| format | Article |
| id | doaj-art-2afbdd8767f549339e4af9e696f96050 |
| institution | DOAJ |
| issn | 1305-6441 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | İstanbul Tıp Fakültesi Dergisi |
| spelling | doaj-art-2afbdd8767f549339e4af9e696f960502025-08-20T02:57:43ZengIstanbul University Pressİstanbul Tıp Fakültesi Dergisi1305-64412021-01-0184111311910.26650/IUITFD.2020.0009123456MALDI-TOF MS ANALYSIS OF LONG-TERM STORED STOCK CANDIDA STRAINS IDENTIFIED BY MOLECULAR AND CONVENTIONAL METHODSTuğrul Hoşbul0https://orcid.org/0000-0002-0150-4417Fatih Şahiner1https://orcid.org/0000-0002-3488-0339Ramazan Gümral2https://orcid.org/0000-0002-2303-8234Sinem Kaya3https://orcid.org/0000-0003-3911-5342Bayhan Bektöre4https://orcid.org/0000-0002-1225-7693Kemal Tekin5https://orcid.org/0000-0002-6610-6540Şinasi Taner Yıldıran6https://orcid.org/0000-0003-3897-4401Sağlık Bilimleri Üniversitesi, Istanbul, TurkiyeSağlık Bilimleri Üniversitesi, Istanbul, TurkiyeSağlık Bilimleri Üniversitesi, Istanbul, TurkiyeSağlık Bilimleri Üniversitesi, Istanbul, TurkiyeAlanya Alaaddin Keykubat Üniversitesi, Antalya, TurkiyeSağlık Bilimleri Üniversitesi, Istanbul, TurkiyeSağlık Bilimleri Üniversitesi, Istanbul, TurkiyeObjective: In recent years, the rapid and accurate identification of Candida isolates at species level has been of great importance for reasons such as increased incidence of infections due to Candida species and differing antifungal susceptibilities by species. Herein, we aimed to investigate the efficacy of the MALDI-TOF MS method in the identification of Candida clinical isolates stored at -20°C for years. Material and Method: Twenty eight Candida strains stored at -20°C for more than 10 years without any recultivation and identified by various methods such as API® ID 32C, CHROMagar Candida and PCR-RFLP were included in the study. Isolates were subcultured and analysed by MALDI-TOF MS. An unidentified isolate at the species level previously and another isolate with low MALDI-TOF MS score were analyzed by ITS sequencing. Additionally, MALDI-TOF MS scores of 57 Candida strains isolated from routine cultures were compared with study isolates. Results: Of them, 11 (39.3%) isolates were identified at the species level and 15 (53.6%) were identified at the genus level, while two isolates were outside the reliable identification limits by MALDI-TOF MS. MALDI-TOF MS yielded an accurate identification at the species level in all 28 isolates identified by various methods even though different scores were obtained. Conclusion: MALDI-TOF MS is a cost effective, easy to use, fast and reliable method compared with the conventional and molecular methods for identification of stock Candida isolates preserved for many years. In conclusion, although a limited number of stock Candida isolates were included in the study, low identification scores for Candida isolates may be valuable in the MALDI-TOF MS analysis.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/AB86A576A6D54B33BD8B8D6904467B04candidamaldi-tof msidentification |
| spellingShingle | Tuğrul Hoşbul Fatih Şahiner Ramazan Gümral Sinem Kaya Bayhan Bektöre Kemal Tekin Şinasi Taner Yıldıran MALDI-TOF MS ANALYSIS OF LONG-TERM STORED STOCK CANDIDA STRAINS IDENTIFIED BY MOLECULAR AND CONVENTIONAL METHODS İstanbul Tıp Fakültesi Dergisi candida maldi-tof ms identification |
| title | MALDI-TOF MS ANALYSIS OF LONG-TERM STORED STOCK CANDIDA STRAINS IDENTIFIED BY MOLECULAR AND CONVENTIONAL METHODS |
| title_full | MALDI-TOF MS ANALYSIS OF LONG-TERM STORED STOCK CANDIDA STRAINS IDENTIFIED BY MOLECULAR AND CONVENTIONAL METHODS |
| title_fullStr | MALDI-TOF MS ANALYSIS OF LONG-TERM STORED STOCK CANDIDA STRAINS IDENTIFIED BY MOLECULAR AND CONVENTIONAL METHODS |
| title_full_unstemmed | MALDI-TOF MS ANALYSIS OF LONG-TERM STORED STOCK CANDIDA STRAINS IDENTIFIED BY MOLECULAR AND CONVENTIONAL METHODS |
| title_short | MALDI-TOF MS ANALYSIS OF LONG-TERM STORED STOCK CANDIDA STRAINS IDENTIFIED BY MOLECULAR AND CONVENTIONAL METHODS |
| title_sort | maldi tof ms analysis of long term stored stock candida strains identified by molecular and conventional methods |
| topic | candida maldi-tof ms identification |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/AB86A576A6D54B33BD8B8D6904467B04 |
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