Monitoring and assessment of SARS-CoV-2 evolution
The aim of the study – systematization and analysis of information on the prevalence of SARS-CoV-2 virus variants according to information databases and own cross-sectional studies. Materials and methods. The analysis was conducted in the period 2020–2021 using information databases and research...
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Zaporizhzhia State Medical and Pharmaceutical University
2022-02-01
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| Series: | Zaporožskij Medicinskij Žurnal |
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| Online Access: | http://zmj.zsmu.edu.ua/article/view/241658/250050 |
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| author | V. V. Miasoiedov P. V. Nartov K. V. Yurko V. M. Lesovoy V. A. Kapustnyk A. V. Bondarenko M. Ye. Cherniak V. A. Yakushchenko V. V. Kucheriavchenko V. S. Maslova O. V. Bondarenko |
| author_facet | V. V. Miasoiedov P. V. Nartov K. V. Yurko V. M. Lesovoy V. A. Kapustnyk A. V. Bondarenko M. Ye. Cherniak V. A. Yakushchenko V. V. Kucheriavchenko V. S. Maslova O. V. Bondarenko |
| author_sort | V. V. Miasoiedov |
| collection | DOAJ |
| description | The aim of the study – systematization and analysis of information on the prevalence of SARS-CoV-2 virus variants according to information databases and own cross-sectional studies.
Materials and methods. The analysis was conducted in the period 2020–2021 using information databases and research resources: Google Scholar, Cochrane Library, Scirus, Springer, Medline, Embase, PubMed, Web of Science. Our own examination included a total of 80 nasopharyngeal swabs (at least two specimens in each case) collected from ARVI symptomatic or suspected COVID-19 patients, who were treated at the MNE KRC “Regional Clinical Infectious Diseases Hospital” from June to October 2021 and tested positive on SARS-CoV-2. The first study was performed in the inter-peak period, and the second one – on the rise of the disease. A real-time polymerase chain reaction (PCR) method was used for primary identification with the diagnostic kit “Biocore® SARS-CoV-2” (LLC “Biocor Technology Ltd.”, Ukraine) in accordance with the manufacturer’s instructions. Multiplex analysis SNPsig® VariPLEX™ Covid-19 (Primerdesign™ Ltd., UK) was used in the first cross-sectional study for secondary identification.
Results. Systematization and analysis of the prevalence of SARS-CoV-2 variants according to information databases were performed. According to the results of our cross-sectional studies on SARS-CoV2 variants circulating in the Kharkiv region in June – October 2021, the complete replacement of the alpha variant, which was dominant in June – 87.5 %, with the delta variant – 95.0 %. All patients had signs of respiratory failure, community-acquired pneumonia was visualized instrumentally (computed tomography, radiography, ultrasound) at the first study in 90 % of cases, and at the second – in 100 %. The median age of patients was 60.4 years (minimum – 25 years, maximum – 91 years) during the first study, but during the second – 52.6 years (minimum – 18 years, maximum – 84 years). Thus, the more aggressive and contagious delta variant has become dominant requiring thorough public health anti-epidemic measures.
Conclusions. Monitoring and control of the virus evolution using epidemiological studies, viral genetic sequence, as well as laboratory PCR are necessary to prevent the spread of COVID-19, to study the effectiveness of test systems and optimize diagnosis and etiotropic therapy as well as vaccine modifications.
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| format | Article |
| id | doaj-art-2af464ee239c4391968ed4416ee9f025 |
| institution | DOAJ |
| issn | 2306-4145 2310-1210 |
| language | English |
| publishDate | 2022-02-01 |
| publisher | Zaporizhzhia State Medical and Pharmaceutical University |
| record_format | Article |
| series | Zaporožskij Medicinskij Žurnal |
| spelling | doaj-art-2af464ee239c4391968ed4416ee9f0252025-08-20T03:17:36ZengZaporizhzhia State Medical and Pharmaceutical UniversityZaporožskij Medicinskij Žurnal2306-41452310-12102022-02-0124110911410.14739/2310-1210.2022.1.241658Monitoring and assessment of SARS-CoV-2 evolutionV. V. Miasoiedov0https://orcid.org/0000-0001-7135-4672P. V. Nartov1https://orcid.org/0000-0001-8292-8192K. V. Yurko2https://orcid.org/0000-0002-1226-5431V. M. Lesovoy3https://orcid.org/0000-0002-2301-2229V. A. Kapustnyk4https://orcid.org/0000-0002-4543-8343A. V. Bondarenko5https://orcid.org/0000-0002-2303-8525M. Ye. Cherniak6https://orcid.org/0000-0002-7425-659XV. A. Yakushchenko7https://orcid.org/0000-0002-5967-9228V. V. Kucheriavchenko8https://orcid.org/0000-0001-9360-8258V. S. Maslova9https://orcid.org/0000-0002-5967-9228O. V. Bondarenko10https://orcid.org/0000-0003-1755-925XKharkiv National Medical University, UkraineMunicipal Nonprofit Enterprise of Kharkiv Regional Council “Regional Clinical Infectious Hospital”, UkraineKharkiv National Medical University, UkraineKharkiv National Medical University, UkraineKharkiv National Medical University, UkraineKharkiv National Medical University, UkraineKharkiv National Medical University, UkraineNational University of Pharmacy, Kharkiv, UkraineKharkiv National Medical University, UkraineKharkiv Regional Council “Regional Clinical Infectious Hospital”, UkraineKharkiv Regional Council “Regional Clinical Infectious Hospital”, UkraineThe aim of the study – systematization and analysis of information on the prevalence of SARS-CoV-2 virus variants according to information databases and own cross-sectional studies. Materials and methods. The analysis was conducted in the period 2020–2021 using information databases and research resources: Google Scholar, Cochrane Library, Scirus, Springer, Medline, Embase, PubMed, Web of Science. Our own examination included a total of 80 nasopharyngeal swabs (at least two specimens in each case) collected from ARVI symptomatic or suspected COVID-19 patients, who were treated at the MNE KRC “Regional Clinical Infectious Diseases Hospital” from June to October 2021 and tested positive on SARS-CoV-2. The first study was performed in the inter-peak period, and the second one – on the rise of the disease. A real-time polymerase chain reaction (PCR) method was used for primary identification with the diagnostic kit “Biocore® SARS-CoV-2” (LLC “Biocor Technology Ltd.”, Ukraine) in accordance with the manufacturer’s instructions. Multiplex analysis SNPsig® VariPLEX™ Covid-19 (Primerdesign™ Ltd., UK) was used in the first cross-sectional study for secondary identification. Results. Systematization and analysis of the prevalence of SARS-CoV-2 variants according to information databases were performed. According to the results of our cross-sectional studies on SARS-CoV2 variants circulating in the Kharkiv region in June – October 2021, the complete replacement of the alpha variant, which was dominant in June – 87.5 %, with the delta variant – 95.0 %. All patients had signs of respiratory failure, community-acquired pneumonia was visualized instrumentally (computed tomography, radiography, ultrasound) at the first study in 90 % of cases, and at the second – in 100 %. The median age of patients was 60.4 years (minimum – 25 years, maximum – 91 years) during the first study, but during the second – 52.6 years (minimum – 18 years, maximum – 84 years). Thus, the more aggressive and contagious delta variant has become dominant requiring thorough public health anti-epidemic measures. Conclusions. Monitoring and control of the virus evolution using epidemiological studies, viral genetic sequence, as well as laboratory PCR are necessary to prevent the spread of COVID-19, to study the effectiveness of test systems and optimize diagnosis and etiotropic therapy as well as vaccine modifications. http://zmj.zsmu.edu.ua/article/view/241658/250050covid-19sars-cov-2genetic variability |
| spellingShingle | V. V. Miasoiedov P. V. Nartov K. V. Yurko V. M. Lesovoy V. A. Kapustnyk A. V. Bondarenko M. Ye. Cherniak V. A. Yakushchenko V. V. Kucheriavchenko V. S. Maslova O. V. Bondarenko Monitoring and assessment of SARS-CoV-2 evolution Zaporožskij Medicinskij Žurnal covid-19 sars-cov-2 genetic variability |
| title | Monitoring and assessment of SARS-CoV-2 evolution |
| title_full | Monitoring and assessment of SARS-CoV-2 evolution |
| title_fullStr | Monitoring and assessment of SARS-CoV-2 evolution |
| title_full_unstemmed | Monitoring and assessment of SARS-CoV-2 evolution |
| title_short | Monitoring and assessment of SARS-CoV-2 evolution |
| title_sort | monitoring and assessment of sars cov 2 evolution |
| topic | covid-19 sars-cov-2 genetic variability |
| url | http://zmj.zsmu.edu.ua/article/view/241658/250050 |
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