MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wnt/β-Cateninsignaling Pathway
Gastric cancer is one of the most frequently diagnosed cancer and poses a serious threat to health system in the world. Upregulation of meningioma-associated protein (MAC30) has been found in many solid tumors and can regulate the proliferation, differentiation, and apoptosis of different tumor cell...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2020/6358685 |
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| author | Xiaohong Wu Yong Zhang Junping Guo Xun Yan Li Shen Jianping Zhou Jun Zhao Min Zhuang Zhihong Cao |
| author_facet | Xiaohong Wu Yong Zhang Junping Guo Xun Yan Li Shen Jianping Zhou Jun Zhao Min Zhuang Zhihong Cao |
| author_sort | Xiaohong Wu |
| collection | DOAJ |
| description | Gastric cancer is one of the most frequently diagnosed cancer and poses a serious threat to health system in the world. Upregulation of meningioma-associated protein (MAC30) has been found in many solid tumors and can regulate the proliferation, differentiation, and apoptosis of different tumor cells. Quantitative polymerase chain reaction (qPCR) was used to detect the expression of MAC30 in 68 patients with gastric cancer and their adjacent tissues. Lentiviral vector pGCSIL-shMAC30-GFP of the RNA interference (RNAi) of the MAC30 gene was transfected into gastric cancer BGC-823 cell line and the expression of lentivirus label protein GFP was observed via fluorescence microscope, while cell proliferation and apoptosis were determined with flow cytometry and MTT assay, respectively. Also, related protein expressions on Wnt/β-catenin signaling pathway were analyzed by Western blot method. The expression of MAC30 was abnormally elevated in gastric cancer tissues, while interfering of its expression could significantly inhibit the proliferation of gastric cancer BGC-823 cell line. However, the promotion of apoptosis by mitochondrial pathway was mediated by Bax/Bcl-2 upregulation. Present work showed the effect of downregulated MAC30 expression on proliferation and apoptosis of gastric cancer cell through Wnt/β-catenin signaling pathway. Thus, this investigation provides an experimental basis for future development of chemotherapeutic agent on gastric cancer. |
| format | Article |
| id | doaj-art-2ae3b037ea0846619f9a90c05b8e7e60 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-2ae3b037ea0846619f9a90c05b8e7e602025-02-03T00:58:42ZengWileyGastroenterology Research and Practice1687-61211687-630X2020-01-01202010.1155/2020/63586856358685MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wnt/β-Cateninsignaling PathwayXiaohong Wu0Yong Zhang1Junping Guo2Xun Yan3Li Shen4Jianping Zhou5Jun Zhao6Min Zhuang7Zhihong Cao8Department of General Surgery, the Affiliated Yixing Hospital of Jiangsu University, Yixing 214200, ChinaDepartment of Pharmacy, the Affiliated Yixing Hospital of Jiangsu University, Yixing 214200, ChinaDepartment of General Surgery, Binhai County People’s Hospital of Jiangsu Province, Binhai 224500, ChinaDisinfection Supply Center, the Affiliated Yixing Hospital of Jiangsu University, Yixing 214200, ChinaDepartment of Gastrointestinal Surgery, the Affiliated Yixing Hospital of Jiangsu University, Yixing 214200, ChinaDepartment of Chest Surgery, Liyang People’s Hospital, Liyang 213371, ChinaDepartment of Radiology, the Affiliated Yixing Hospital of Jiangsu University, Yixing 214200, ChinaDeputy Chief Inspector of Laboratory Division, the Affiliated Yixing Hospital of Jiangsu University, Yixing 214200, ChinaDepartment of Gastroenterology, the Affiliated Yixing Hospital of Jiangsu University, Yixing 214200, ChinaGastric cancer is one of the most frequently diagnosed cancer and poses a serious threat to health system in the world. Upregulation of meningioma-associated protein (MAC30) has been found in many solid tumors and can regulate the proliferation, differentiation, and apoptosis of different tumor cells. Quantitative polymerase chain reaction (qPCR) was used to detect the expression of MAC30 in 68 patients with gastric cancer and their adjacent tissues. Lentiviral vector pGCSIL-shMAC30-GFP of the RNA interference (RNAi) of the MAC30 gene was transfected into gastric cancer BGC-823 cell line and the expression of lentivirus label protein GFP was observed via fluorescence microscope, while cell proliferation and apoptosis were determined with flow cytometry and MTT assay, respectively. Also, related protein expressions on Wnt/β-catenin signaling pathway were analyzed by Western blot method. The expression of MAC30 was abnormally elevated in gastric cancer tissues, while interfering of its expression could significantly inhibit the proliferation of gastric cancer BGC-823 cell line. However, the promotion of apoptosis by mitochondrial pathway was mediated by Bax/Bcl-2 upregulation. Present work showed the effect of downregulated MAC30 expression on proliferation and apoptosis of gastric cancer cell through Wnt/β-catenin signaling pathway. Thus, this investigation provides an experimental basis for future development of chemotherapeutic agent on gastric cancer.http://dx.doi.org/10.1155/2020/6358685 |
| spellingShingle | Xiaohong Wu Yong Zhang Junping Guo Xun Yan Li Shen Jianping Zhou Jun Zhao Min Zhuang Zhihong Cao MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wnt/β-Cateninsignaling Pathway Gastroenterology Research and Practice |
| title | MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wnt/β-Cateninsignaling Pathway |
| title_full | MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wnt/β-Cateninsignaling Pathway |
| title_fullStr | MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wnt/β-Cateninsignaling Pathway |
| title_full_unstemmed | MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wnt/β-Cateninsignaling Pathway |
| title_short | MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wnt/β-Cateninsignaling Pathway |
| title_sort | mac30 knockdown inhibits proliferation and enhance apoptosis of gastric cancer by suppressing wnt β cateninsignaling pathway |
| url | http://dx.doi.org/10.1155/2020/6358685 |
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