The cellular distribution of P2X7, P2Y6, and P2Y12 during or after pilocarpine-induced status epilepticus and literature review
BACKGROUND: When a seizure occurs, the distribution of purine receptors in different cell types at various time points remains poorly understood. Our literature review revealed that P2X7, P2Y6, and P2Y12 are expressed in different cells during epilepsy pathogenesis. Therefore, we studied the protein...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2024-12-01
|
| Series: | Brain Circulation |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/bc.bc_27_24 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841556921616695296 |
|---|---|
| author | Yue Li Fengru Tang Yumin Luo |
| author_facet | Yue Li Fengru Tang Yumin Luo |
| author_sort | Yue Li |
| collection | DOAJ |
| description | BACKGROUND:
When a seizure occurs, the distribution of purine receptors in different cell types at various time points remains poorly understood. Our literature review revealed that P2X7, P2Y6, and P2Y12 are expressed in different cells during epilepsy pathogenesis. Therefore, we studied the protein expression patterns of the purinergic receptors P2X7, P2Y6, and P2Y12 in the normal mice hippocampus, as well as during or after pilocarpine-induced status epilepticus (DPISE or APISE).
MATERIALS AND METHODS:
Immunohistochemical staining and double-labeling immunofluorescence staining were used to study the cellular distribution of various purinergic receptors across several groups: control, 2-hour DPISE, 1-day APISE, 2-day APISE, 3-day APISE, and 1-week APISE.
RESULTS:
In the normal mouse brain, P2X7, P2Y6, and P2Y12 were predominantly expressed in the neurons. Microglia and astrocytes were found to express these receptors at the onset of seizures. Immunofluorescence analysis showed that P2X7 and P2Y12 are expressed in microglia, whereas P2Y6 is mainly expressed in astrocytes.
CONCLUSION:
Different purinergic receptors are expressed in neurons, microglia, and astrocytes, mediate their interactions, and are involved in epileptogenesis. |
| format | Article |
| id | doaj-art-2adfa30ca81840489c7b779ea1fd5a5c |
| institution | Kabale University |
| issn | 2455-4626 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Brain Circulation |
| spelling | doaj-art-2adfa30ca81840489c7b779ea1fd5a5c2025-01-07T05:14:06ZengWolters Kluwer Medknow PublicationsBrain Circulation2455-46262024-12-0110434335310.4103/bc.bc_27_24The cellular distribution of P2X7, P2Y6, and P2Y12 during or after pilocarpine-induced status epilepticus and literature reviewYue LiFengru TangYumin LuoBACKGROUND: When a seizure occurs, the distribution of purine receptors in different cell types at various time points remains poorly understood. Our literature review revealed that P2X7, P2Y6, and P2Y12 are expressed in different cells during epilepsy pathogenesis. Therefore, we studied the protein expression patterns of the purinergic receptors P2X7, P2Y6, and P2Y12 in the normal mice hippocampus, as well as during or after pilocarpine-induced status epilepticus (DPISE or APISE). MATERIALS AND METHODS: Immunohistochemical staining and double-labeling immunofluorescence staining were used to study the cellular distribution of various purinergic receptors across several groups: control, 2-hour DPISE, 1-day APISE, 2-day APISE, 3-day APISE, and 1-week APISE. RESULTS: In the normal mouse brain, P2X7, P2Y6, and P2Y12 were predominantly expressed in the neurons. Microglia and astrocytes were found to express these receptors at the onset of seizures. Immunofluorescence analysis showed that P2X7 and P2Y12 are expressed in microglia, whereas P2Y6 is mainly expressed in astrocytes. CONCLUSION: Different purinergic receptors are expressed in neurons, microglia, and astrocytes, mediate their interactions, and are involved in epileptogenesis.https://journals.lww.com/10.4103/bc.bc_27_24astrocytesmicroglianeuronp2x7p2y12p2y6purinergic receptorsstatus epilepticus |
| spellingShingle | Yue Li Fengru Tang Yumin Luo The cellular distribution of P2X7, P2Y6, and P2Y12 during or after pilocarpine-induced status epilepticus and literature review Brain Circulation astrocytes microglia neuron p2x7 p2y12 p2y6 purinergic receptors status epilepticus |
| title | The cellular distribution of P2X7, P2Y6, and P2Y12 during or after pilocarpine-induced status epilepticus and literature review |
| title_full | The cellular distribution of P2X7, P2Y6, and P2Y12 during or after pilocarpine-induced status epilepticus and literature review |
| title_fullStr | The cellular distribution of P2X7, P2Y6, and P2Y12 during or after pilocarpine-induced status epilepticus and literature review |
| title_full_unstemmed | The cellular distribution of P2X7, P2Y6, and P2Y12 during or after pilocarpine-induced status epilepticus and literature review |
| title_short | The cellular distribution of P2X7, P2Y6, and P2Y12 during or after pilocarpine-induced status epilepticus and literature review |
| title_sort | cellular distribution of p2x7 p2y6 and p2y12 during or after pilocarpine induced status epilepticus and literature review |
| topic | astrocytes microglia neuron p2x7 p2y12 p2y6 purinergic receptors status epilepticus |
| url | https://journals.lww.com/10.4103/bc.bc_27_24 |
| work_keys_str_mv | AT yueli thecellulardistributionofp2x7p2y6andp2y12duringorafterpilocarpineinducedstatusepilepticusandliteraturereview AT fengrutang thecellulardistributionofp2x7p2y6andp2y12duringorafterpilocarpineinducedstatusepilepticusandliteraturereview AT yuminluo thecellulardistributionofp2x7p2y6andp2y12duringorafterpilocarpineinducedstatusepilepticusandliteraturereview AT yueli cellulardistributionofp2x7p2y6andp2y12duringorafterpilocarpineinducedstatusepilepticusandliteraturereview AT fengrutang cellulardistributionofp2x7p2y6andp2y12duringorafterpilocarpineinducedstatusepilepticusandliteraturereview AT yuminluo cellulardistributionofp2x7p2y6andp2y12duringorafterpilocarpineinducedstatusepilepticusandliteraturereview |