The complete genome sequence of Haloferax volcanii DS2, a model archaeon.

<h4>Background</h4>Haloferax volcanii is an easily culturable moderate halophile that grows on simple defined media, is readily transformable, and has a relatively stable genome. This, in combination with its biochemical and genetic tractability, has made Hfx. volcanii a key model organi...

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Main Authors: Amber L Hartman, Cédric Norais, Jonathan H Badger, Stéphane Delmas, Sam Haldenby, Ramana Madupu, Jeffrey Robinson, Hoda Khouri, Qinghu Ren, Todd M Lowe, Julie Maupin-Furlow, Mecky Pohlschroder, Charles Daniels, Friedhelm Pfeiffer, Thorsten Allers, Jonathan A Eisen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-03-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0009605&type=printable
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author Amber L Hartman
Cédric Norais
Jonathan H Badger
Stéphane Delmas
Sam Haldenby
Ramana Madupu
Jeffrey Robinson
Hoda Khouri
Qinghu Ren
Todd M Lowe
Julie Maupin-Furlow
Mecky Pohlschroder
Charles Daniels
Friedhelm Pfeiffer
Thorsten Allers
Jonathan A Eisen
author_facet Amber L Hartman
Cédric Norais
Jonathan H Badger
Stéphane Delmas
Sam Haldenby
Ramana Madupu
Jeffrey Robinson
Hoda Khouri
Qinghu Ren
Todd M Lowe
Julie Maupin-Furlow
Mecky Pohlschroder
Charles Daniels
Friedhelm Pfeiffer
Thorsten Allers
Jonathan A Eisen
author_sort Amber L Hartman
collection DOAJ
description <h4>Background</h4>Haloferax volcanii is an easily culturable moderate halophile that grows on simple defined media, is readily transformable, and has a relatively stable genome. This, in combination with its biochemical and genetic tractability, has made Hfx. volcanii a key model organism, not only for the study of halophilicity, but also for archaeal biology in general.<h4>Methodology/principal findings</h4>We report here the sequencing and analysis of the genome of Hfx. volcanii DS2, the type strain of this species. The genome contains a main 2.848 Mb chromosome, three smaller chromosomes pHV1, 3, 4 (85, 438, 636 kb, respectively) and the pHV2 plasmid (6.4 kb).<h4>Conclusions/significance</h4>The completed genome sequence, presented here, provides an invaluable tool for further in vivo and in vitro studies of Hfx. volcanii.
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spelling doaj-art-2ad8b076930a40b983adb704aff420cd2025-08-20T03:07:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-03-0153e960510.1371/journal.pone.0009605The complete genome sequence of Haloferax volcanii DS2, a model archaeon.Amber L HartmanCédric NoraisJonathan H BadgerStéphane DelmasSam HaldenbyRamana MadupuJeffrey RobinsonHoda KhouriQinghu RenTodd M LoweJulie Maupin-FurlowMecky PohlschroderCharles DanielsFriedhelm PfeifferThorsten AllersJonathan A Eisen<h4>Background</h4>Haloferax volcanii is an easily culturable moderate halophile that grows on simple defined media, is readily transformable, and has a relatively stable genome. This, in combination with its biochemical and genetic tractability, has made Hfx. volcanii a key model organism, not only for the study of halophilicity, but also for archaeal biology in general.<h4>Methodology/principal findings</h4>We report here the sequencing and analysis of the genome of Hfx. volcanii DS2, the type strain of this species. The genome contains a main 2.848 Mb chromosome, three smaller chromosomes pHV1, 3, 4 (85, 438, 636 kb, respectively) and the pHV2 plasmid (6.4 kb).<h4>Conclusions/significance</h4>The completed genome sequence, presented here, provides an invaluable tool for further in vivo and in vitro studies of Hfx. volcanii.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0009605&type=printable
spellingShingle Amber L Hartman
Cédric Norais
Jonathan H Badger
Stéphane Delmas
Sam Haldenby
Ramana Madupu
Jeffrey Robinson
Hoda Khouri
Qinghu Ren
Todd M Lowe
Julie Maupin-Furlow
Mecky Pohlschroder
Charles Daniels
Friedhelm Pfeiffer
Thorsten Allers
Jonathan A Eisen
The complete genome sequence of Haloferax volcanii DS2, a model archaeon.
PLoS ONE
title The complete genome sequence of Haloferax volcanii DS2, a model archaeon.
title_full The complete genome sequence of Haloferax volcanii DS2, a model archaeon.
title_fullStr The complete genome sequence of Haloferax volcanii DS2, a model archaeon.
title_full_unstemmed The complete genome sequence of Haloferax volcanii DS2, a model archaeon.
title_short The complete genome sequence of Haloferax volcanii DS2, a model archaeon.
title_sort complete genome sequence of haloferax volcanii ds2 a model archaeon
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0009605&type=printable
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