A Test-Retest Study of Single- and Multi-Delay pCASL for Choroid Plexus Perfusion Imaging in Healthy Subjects Aged 19 to 87 Years
There is a growing interest in the choroid plexus (ChP) due to its critical role in cerebrospinal fluid (CSF) production and its involvement in neurodegenerative and cerebrovascular diseases. However, comprehensive studies comparing the accuracy and reliability of single- and multi-PLD (post-labelin...
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Elsevier
2025-03-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1053811925000503 |
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| author | Zixuan Liu Qinyang Shou Kay Jann Chenyang Zhao Danny JJ Wang Xingfeng Shao |
| author_facet | Zixuan Liu Qinyang Shou Kay Jann Chenyang Zhao Danny JJ Wang Xingfeng Shao |
| author_sort | Zixuan Liu |
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| description | There is a growing interest in the choroid plexus (ChP) due to its critical role in cerebrospinal fluid (CSF) production and its involvement in neurodegenerative and cerebrovascular diseases. However, comprehensive studies comparing the accuracy and reliability of single- and multi-PLD (post-labeling delay) arterial spin labeling (ASL) techniques, specifically in relation to the ChP, remain limited. This study systematically evaluated the test-retest reliability and quantification accuracy of cerebral blood flow (CBF) measurements, focusing on the ChP, using single-delay and multi-delay 3D gradient-and-spin echo (GRASE) pseudo-continuous ASL (pCASL) on 28 subjects (aged 19 to 87 years, 14 males/14 females) at 3.0 tesla. Both single-delay (2 s) and 5-PLD (0.5 – 2.5 s) pCASL scans were repeated approximately one week apart with a spatial resolution of 2.5 × 2.5 × 3 mm³. Voxel-wise and regional CBF and arterial transit time (ATT) measurements were compared to assess test-retest reliability, with a particular focus on ChP perfusion changes with age. In this study, 12.15 % of ChP voxels exhibited ATTs longer than 2 s, potentially leading to a significant underestimation of CBF using single-delay ASL. Multi-delay ASL showed improved accuracy in estimating CBF values for the ChP compared to single-delay ASL when ATT > PLD. Additionally, ChP volume (mean ± std = 1.72± 0.85 ml) increased (p < 0.01) and ChP perfusion (43.07±14.18 mL/100 g/min) decreased (p = 0.04) with age. These findings underscore the robustness of multi-delay ASL with model-fitting quantification in assessing ChP perfusion, making it the preferred method for accurate CBF and ATT estimation, particularly in regions with prolonged transit time such as ChP. |
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| institution | Kabale University |
| issn | 1095-9572 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | NeuroImage |
| spelling | doaj-art-2acd70f78627499ba90d6186bfca9ad02025-02-05T04:31:13ZengElsevierNeuroImage1095-95722025-03-01308121048A Test-Retest Study of Single- and Multi-Delay pCASL for Choroid Plexus Perfusion Imaging in Healthy Subjects Aged 19 to 87 YearsZixuan Liu0Qinyang Shou1Kay Jann2Chenyang Zhao3Danny JJ Wang4Xingfeng Shao5Laboratory of FMRI Technology (LOFT), Mark & Mary Stevens Neuroimaging and Informatics Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA; Alfred E. Mann Department of Biomedical Engineering, University of Southern California, Los Angeles, CA, USALaboratory of FMRI Technology (LOFT), Mark & Mary Stevens Neuroimaging and Informatics Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA; Alfred E. Mann Department of Biomedical Engineering, University of Southern California, Los Angeles, CA, USALaboratory of FMRI Technology (LOFT), Mark & Mary Stevens Neuroimaging and Informatics Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, USALaboratory of FMRI Technology (LOFT), Mark & Mary Stevens Neuroimaging and Informatics Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA; Alfred E. Mann Department of Biomedical Engineering, University of Southern California, Los Angeles, CA, USALaboratory of FMRI Technology (LOFT), Mark & Mary Stevens Neuroimaging and Informatics Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA; Alfred E. Mann Department of Biomedical Engineering, University of Southern California, Los Angeles, CA, USALaboratory of FMRI Technology (LOFT), Mark & Mary Stevens Neuroimaging and Informatics Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA; Correspondence author at Laboratory of FMRI Technology (LOFT), Mark & Mary Stevens Neuroimaging and Informatics Institute, Keck School of Medicine, University of Southern California (USC)There is a growing interest in the choroid plexus (ChP) due to its critical role in cerebrospinal fluid (CSF) production and its involvement in neurodegenerative and cerebrovascular diseases. However, comprehensive studies comparing the accuracy and reliability of single- and multi-PLD (post-labeling delay) arterial spin labeling (ASL) techniques, specifically in relation to the ChP, remain limited. This study systematically evaluated the test-retest reliability and quantification accuracy of cerebral blood flow (CBF) measurements, focusing on the ChP, using single-delay and multi-delay 3D gradient-and-spin echo (GRASE) pseudo-continuous ASL (pCASL) on 28 subjects (aged 19 to 87 years, 14 males/14 females) at 3.0 tesla. Both single-delay (2 s) and 5-PLD (0.5 – 2.5 s) pCASL scans were repeated approximately one week apart with a spatial resolution of 2.5 × 2.5 × 3 mm³. Voxel-wise and regional CBF and arterial transit time (ATT) measurements were compared to assess test-retest reliability, with a particular focus on ChP perfusion changes with age. In this study, 12.15 % of ChP voxels exhibited ATTs longer than 2 s, potentially leading to a significant underestimation of CBF using single-delay ASL. Multi-delay ASL showed improved accuracy in estimating CBF values for the ChP compared to single-delay ASL when ATT > PLD. Additionally, ChP volume (mean ± std = 1.72± 0.85 ml) increased (p < 0.01) and ChP perfusion (43.07±14.18 mL/100 g/min) decreased (p = 0.04) with age. These findings underscore the robustness of multi-delay ASL with model-fitting quantification in assessing ChP perfusion, making it the preferred method for accurate CBF and ATT estimation, particularly in regions with prolonged transit time such as ChP.http://www.sciencedirect.com/science/article/pii/S1053811925000503Arterial spin labeling (ASL)Single-delay ASLMultiple-delay ASLQuantification accuracyTest-retest reliabilityAging |
| spellingShingle | Zixuan Liu Qinyang Shou Kay Jann Chenyang Zhao Danny JJ Wang Xingfeng Shao A Test-Retest Study of Single- and Multi-Delay pCASL for Choroid Plexus Perfusion Imaging in Healthy Subjects Aged 19 to 87 Years NeuroImage Arterial spin labeling (ASL) Single-delay ASL Multiple-delay ASL Quantification accuracy Test-retest reliability Aging |
| title | A Test-Retest Study of Single- and Multi-Delay pCASL for Choroid Plexus Perfusion Imaging in Healthy Subjects Aged 19 to 87 Years |
| title_full | A Test-Retest Study of Single- and Multi-Delay pCASL for Choroid Plexus Perfusion Imaging in Healthy Subjects Aged 19 to 87 Years |
| title_fullStr | A Test-Retest Study of Single- and Multi-Delay pCASL for Choroid Plexus Perfusion Imaging in Healthy Subjects Aged 19 to 87 Years |
| title_full_unstemmed | A Test-Retest Study of Single- and Multi-Delay pCASL for Choroid Plexus Perfusion Imaging in Healthy Subjects Aged 19 to 87 Years |
| title_short | A Test-Retest Study of Single- and Multi-Delay pCASL for Choroid Plexus Perfusion Imaging in Healthy Subjects Aged 19 to 87 Years |
| title_sort | test retest study of single and multi delay pcasl for choroid plexus perfusion imaging in healthy subjects aged 19 to 87 years |
| topic | Arterial spin labeling (ASL) Single-delay ASL Multiple-delay ASL Quantification accuracy Test-retest reliability Aging |
| url | http://www.sciencedirect.com/science/article/pii/S1053811925000503 |
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