Viral metagenomics reveals diverse viruses in the fecal samples of children with acute respiratory infection

IntroductionChanges in the gut microbiome have been associated with the development of acute respiratory infection (ARI). However, due to methodological limitations, our knowledge of the gut virome in patients with ARIs remains limited.MethodsIn this study, fecal samples from children with ARI were...

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Main Authors: Pan Xu, Chunduo Pan, Minli Yuan, Ying Zhu, Shanjie Wei, Hongyan Lu, Wen Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1564755/full
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author Pan Xu
Chunduo Pan
Minli Yuan
Ying Zhu
Shanjie Wei
Hongyan Lu
Wen Zhang
author_facet Pan Xu
Chunduo Pan
Minli Yuan
Ying Zhu
Shanjie Wei
Hongyan Lu
Wen Zhang
author_sort Pan Xu
collection DOAJ
description IntroductionChanges in the gut microbiome have been associated with the development of acute respiratory infection (ARI). However, due to methodological limitations, our knowledge of the gut virome in patients with ARIs remains limited.MethodsIn this study, fecal samples from children with ARI were investigated using viral metagenomics.ResultsThe fecal virome was analyzed, and several suspected disease-causing viruses were identified. The five viral families with the highest abundance of sequence reads were Podoviridae, Virgaviridae, Siphoviridae, Microviridae, and Myoviridae. Additionally, human adenovirus, human bocavirus, human astrovirus, norovirus, and human rhinovirus were detected. The genome sequences of these viruses were respectively described, and phylogenetic trees were constructed using the gene sequences of the viruses.DiscussionWe characterized the composition of gut virome in children with acute respiratory infections. However, further research is required to elucidate the relationship between acute respiratory infection and gut viruses.
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publisher Frontiers Media S.A.
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series Frontiers in Microbiology
spelling doaj-art-2ac8baedc50246bea79308642d40df562025-08-20T01:54:18ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-04-011610.3389/fmicb.2025.15647551564755Viral metagenomics reveals diverse viruses in the fecal samples of children with acute respiratory infectionPan Xu0Chunduo Pan1Minli Yuan2Ying Zhu3Shanjie Wei4Hongyan Lu5Wen Zhang6Department of Pediatrics, The Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, Jiangsu, ChinaDepartment of Pediatrics, The Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, Jiangsu, ChinaDepartment of Pediatrics, The Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, Jiangsu, ChinaDepartment of Pediatrics, The Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, Jiangsu, ChinaDepartment of Pediatrics, The Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, Jiangsu, ChinaDepartment of Pediatrics, The Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, Jiangsu, ChinaDepartment of Microbiology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, ChinaIntroductionChanges in the gut microbiome have been associated with the development of acute respiratory infection (ARI). However, due to methodological limitations, our knowledge of the gut virome in patients with ARIs remains limited.MethodsIn this study, fecal samples from children with ARI were investigated using viral metagenomics.ResultsThe fecal virome was analyzed, and several suspected disease-causing viruses were identified. The five viral families with the highest abundance of sequence reads were Podoviridae, Virgaviridae, Siphoviridae, Microviridae, and Myoviridae. Additionally, human adenovirus, human bocavirus, human astrovirus, norovirus, and human rhinovirus were detected. The genome sequences of these viruses were respectively described, and phylogenetic trees were constructed using the gene sequences of the viruses.DiscussionWe characterized the composition of gut virome in children with acute respiratory infections. However, further research is required to elucidate the relationship between acute respiratory infection and gut viruses.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1564755/fullacute respiratory infectionchildrenviral metagenomicsfecal samplesvirus evolution
spellingShingle Pan Xu
Chunduo Pan
Minli Yuan
Ying Zhu
Shanjie Wei
Hongyan Lu
Wen Zhang
Viral metagenomics reveals diverse viruses in the fecal samples of children with acute respiratory infection
Frontiers in Microbiology
acute respiratory infection
children
viral metagenomics
fecal samples
virus evolution
title Viral metagenomics reveals diverse viruses in the fecal samples of children with acute respiratory infection
title_full Viral metagenomics reveals diverse viruses in the fecal samples of children with acute respiratory infection
title_fullStr Viral metagenomics reveals diverse viruses in the fecal samples of children with acute respiratory infection
title_full_unstemmed Viral metagenomics reveals diverse viruses in the fecal samples of children with acute respiratory infection
title_short Viral metagenomics reveals diverse viruses in the fecal samples of children with acute respiratory infection
title_sort viral metagenomics reveals diverse viruses in the fecal samples of children with acute respiratory infection
topic acute respiratory infection
children
viral metagenomics
fecal samples
virus evolution
url https://www.frontiersin.org/articles/10.3389/fmicb.2025.1564755/full
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