Biomass Accumulation, Contaminant Removal, and Settling Performance of <i>Chlorella</i> sp. in Unsterilized and Diluted Anaerobic Digestion Effluent
Microalgae demonstrate significant efficacy in wastewater treatment. Anaerobic digestion effluent (ADE) is regarded as an underutilized resource, abundant in carbon, nitrogen, phosphorus, and other nutrients; however, the presence of inhibitory factors restricts microalgal growth, thereby preventing...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/10/11/577 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850145096348991488 |
|---|---|
| author | Canbo Wang Qi Zhang Zhiqiang Gu Longfei Zhang Rumeng Lu Cuixia Liu Yuhuan Liu |
| author_facet | Canbo Wang Qi Zhang Zhiqiang Gu Longfei Zhang Rumeng Lu Cuixia Liu Yuhuan Liu |
| author_sort | Canbo Wang |
| collection | DOAJ |
| description | Microalgae demonstrate significant efficacy in wastewater treatment. Anaerobic digestion effluent (ADE) is regarded as an underutilized resource, abundant in carbon, nitrogen, phosphorus, and other nutrients; however, the presence of inhibitory factors restricts microalgal growth, thereby preventing its direct treatment via microalgae. The purpose of this study was to dilute ADE using various dilution media and subsequently cultivate <i>Chlorella</i> sp. to identify optimal culture conditions that enhance microalgal biomass and water quality. The effects of various dilution conditions were assessed by evaluating the biomass, sedimentation properties, and nutrient removal efficiencies of microalgae. The results demonstrate that microalgal biomass increases as the dilution ratio increased. The microalgae biomass in the treatments diluted with simulated wastewater was significantly higher than that with deionized water, but their effluent quality failed to meet discharge standards. The treatment diluted with deionized water for 10 times exhibited abundant microbial biomass with strong antioxidant properties. The corresponding total phosphorus concentration in the effluent (6.96 mg/L) adhered to emission limits under the Livestock and Poultry Industry Pollutant Emission Standards (8 mg/L), while ammonia nitrogen concentration (90 mg/L) was near compliance (80 mg/L). The corresponding microbial biomass, with a sludge volume index (SVI<sub>30</sub>) of 72.72 mL/g, can be recovered economically and efficiently by simple precipitation. Its high protein (52.07%) and carbohydrate (27.05%) content, coupled with low ash (10.75%), makes it a promising candidate for animal feed and fermentation. This study will aid in understanding microalgal growth in unsterilized ADE and establish a theoretical foundation for cost-effective ADE purification and microalgal biomass production. |
| format | Article |
| id | doaj-art-2ac14a1ca6af4640accc84b20484c022 |
| institution | OA Journals |
| issn | 2311-5637 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj-art-2ac14a1ca6af4640accc84b20484c0222025-08-20T02:28:10ZengMDPI AGFermentation2311-56372024-11-01101157710.3390/fermentation10110577Biomass Accumulation, Contaminant Removal, and Settling Performance of <i>Chlorella</i> sp. in Unsterilized and Diluted Anaerobic Digestion EffluentCanbo Wang0Qi Zhang1Zhiqiang Gu2Longfei Zhang3Rumeng Lu4Cuixia Liu5Yuhuan Liu6State Key Laboratory of Food Science and Resources, Engineering Research Center for Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, Engineering Research Center for Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, Engineering Research Center for Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, Engineering Research Center for Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, Engineering Research Center for Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, ChinaSchool of Energy & Environment, Zhongyuan University of Technology, Zhengzhou 450007, ChinaState Key Laboratory of Food Science and Resources, Engineering Research Center for Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, ChinaMicroalgae demonstrate significant efficacy in wastewater treatment. Anaerobic digestion effluent (ADE) is regarded as an underutilized resource, abundant in carbon, nitrogen, phosphorus, and other nutrients; however, the presence of inhibitory factors restricts microalgal growth, thereby preventing its direct treatment via microalgae. The purpose of this study was to dilute ADE using various dilution media and subsequently cultivate <i>Chlorella</i> sp. to identify optimal culture conditions that enhance microalgal biomass and water quality. The effects of various dilution conditions were assessed by evaluating the biomass, sedimentation properties, and nutrient removal efficiencies of microalgae. The results demonstrate that microalgal biomass increases as the dilution ratio increased. The microalgae biomass in the treatments diluted with simulated wastewater was significantly higher than that with deionized water, but their effluent quality failed to meet discharge standards. The treatment diluted with deionized water for 10 times exhibited abundant microbial biomass with strong antioxidant properties. The corresponding total phosphorus concentration in the effluent (6.96 mg/L) adhered to emission limits under the Livestock and Poultry Industry Pollutant Emission Standards (8 mg/L), while ammonia nitrogen concentration (90 mg/L) was near compliance (80 mg/L). The corresponding microbial biomass, with a sludge volume index (SVI<sub>30</sub>) of 72.72 mL/g, can be recovered economically and efficiently by simple precipitation. Its high protein (52.07%) and carbohydrate (27.05%) content, coupled with low ash (10.75%), makes it a promising candidate for animal feed and fermentation. This study will aid in understanding microalgal growth in unsterilized ADE and establish a theoretical foundation for cost-effective ADE purification and microalgal biomass production.https://www.mdpi.com/2311-5637/10/11/577anaerobic digestion effluentsmicroalgaewastewater treatmentnutrient recoverysedimentation performance |
| spellingShingle | Canbo Wang Qi Zhang Zhiqiang Gu Longfei Zhang Rumeng Lu Cuixia Liu Yuhuan Liu Biomass Accumulation, Contaminant Removal, and Settling Performance of <i>Chlorella</i> sp. in Unsterilized and Diluted Anaerobic Digestion Effluent Fermentation anaerobic digestion effluents microalgae wastewater treatment nutrient recovery sedimentation performance |
| title | Biomass Accumulation, Contaminant Removal, and Settling Performance of <i>Chlorella</i> sp. in Unsterilized and Diluted Anaerobic Digestion Effluent |
| title_full | Biomass Accumulation, Contaminant Removal, and Settling Performance of <i>Chlorella</i> sp. in Unsterilized and Diluted Anaerobic Digestion Effluent |
| title_fullStr | Biomass Accumulation, Contaminant Removal, and Settling Performance of <i>Chlorella</i> sp. in Unsterilized and Diluted Anaerobic Digestion Effluent |
| title_full_unstemmed | Biomass Accumulation, Contaminant Removal, and Settling Performance of <i>Chlorella</i> sp. in Unsterilized and Diluted Anaerobic Digestion Effluent |
| title_short | Biomass Accumulation, Contaminant Removal, and Settling Performance of <i>Chlorella</i> sp. in Unsterilized and Diluted Anaerobic Digestion Effluent |
| title_sort | biomass accumulation contaminant removal and settling performance of i chlorella i sp in unsterilized and diluted anaerobic digestion effluent |
| topic | anaerobic digestion effluents microalgae wastewater treatment nutrient recovery sedimentation performance |
| url | https://www.mdpi.com/2311-5637/10/11/577 |
| work_keys_str_mv | AT canbowang biomassaccumulationcontaminantremovalandsettlingperformanceofichlorellaispinunsterilizedanddilutedanaerobicdigestioneffluent AT qizhang biomassaccumulationcontaminantremovalandsettlingperformanceofichlorellaispinunsterilizedanddilutedanaerobicdigestioneffluent AT zhiqianggu biomassaccumulationcontaminantremovalandsettlingperformanceofichlorellaispinunsterilizedanddilutedanaerobicdigestioneffluent AT longfeizhang biomassaccumulationcontaminantremovalandsettlingperformanceofichlorellaispinunsterilizedanddilutedanaerobicdigestioneffluent AT rumenglu biomassaccumulationcontaminantremovalandsettlingperformanceofichlorellaispinunsterilizedanddilutedanaerobicdigestioneffluent AT cuixialiu biomassaccumulationcontaminantremovalandsettlingperformanceofichlorellaispinunsterilizedanddilutedanaerobicdigestioneffluent AT yuhuanliu biomassaccumulationcontaminantremovalandsettlingperformanceofichlorellaispinunsterilizedanddilutedanaerobicdigestioneffluent |