Herbal Substance, Acteoside, Alleviates Intestinal Mucositis in Mice
This study investigated the role of acteoside in the amelioration of mucositis. C57BL/6 mice were gavaged daily with acteoside 600 μg for 5 d prior to induction of mucositis and throughout the experimental period. Mucositis was induced by methotrexate (MTX; 12.5 mg/kg; s.c.). Mice were culled on d 5...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2015/327872 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558374075498496 |
|---|---|
| author | Daniel Reinke Stamatiki Kritas Panagiotis Polychronopoulos Alexios L. Skaltsounis Nektarios Aligiannis Cuong D. Tran |
| author_facet | Daniel Reinke Stamatiki Kritas Panagiotis Polychronopoulos Alexios L. Skaltsounis Nektarios Aligiannis Cuong D. Tran |
| author_sort | Daniel Reinke |
| collection | DOAJ |
| description | This study investigated the role of acteoside in the amelioration of mucositis. C57BL/6 mice were gavaged daily with acteoside 600 μg for 5 d prior to induction of mucositis and throughout the experimental period. Mucositis was induced by methotrexate (MTX; 12.5 mg/kg; s.c.). Mice were culled on d 5 and d 11 after MTX. The duodenum, jejunum, and ileum were collected for myeloperoxidase (MPO) activity, metallothionein (MT) levels, and histology. Acteoside reduced histological severity scores by 75, 78, and 88% in the duodenum, jejunum, and ileum, respectively, compared to MTX-controls on d 5. Acteoside reduced crypt depth by 49, 51, and 33% and increased villus height by 19, 38, and 10% in the duodenum, jejunum, and ileum, respectively, compared to MTX-controls on d 5. Acteoside decreased MT by 50% compared to MTX-control mice on d 5. Acteoside decreased MPO by 60% and 30% in the duodenum and jejunum, respectively, compared to MTX-controls on d 5. Acteoside alleviated MTX-induced small intestinal mucositis possibly by preventing inflammation. |
| format | Article |
| id | doaj-art-2abf0d25468e41c6bbee08528ec6fbc3 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-2abf0d25468e41c6bbee08528ec6fbc32025-02-03T01:32:32ZengWileyGastroenterology Research and Practice1687-61211687-630X2015-01-01201510.1155/2015/327872327872Herbal Substance, Acteoside, Alleviates Intestinal Mucositis in MiceDaniel Reinke0Stamatiki Kritas1Panagiotis Polychronopoulos2Alexios L. Skaltsounis3Nektarios Aligiannis4Cuong D. Tran5Gastroenterology Unit, Women’s & Children’s Health Network, 72 King William Road, North Adelaide, SA 5006, AustraliaGastroenterology Unit, Women’s & Children’s Health Network, 72 King William Road, North Adelaide, SA 5006, AustraliaDepartment of Pharmacognosy & Natural Products Chemistry, National and Kapodistrian University of Athens, 15771 Ilisia, GreeceDepartment of Pharmacognosy & Natural Products Chemistry, National and Kapodistrian University of Athens, 15771 Ilisia, GreeceDepartment of Pharmacognosy & Natural Products Chemistry, National and Kapodistrian University of Athens, 15771 Ilisia, GreeceGastroenterology Unit, Women’s & Children’s Health Network, 72 King William Road, North Adelaide, SA 5006, AustraliaThis study investigated the role of acteoside in the amelioration of mucositis. C57BL/6 mice were gavaged daily with acteoside 600 μg for 5 d prior to induction of mucositis and throughout the experimental period. Mucositis was induced by methotrexate (MTX; 12.5 mg/kg; s.c.). Mice were culled on d 5 and d 11 after MTX. The duodenum, jejunum, and ileum were collected for myeloperoxidase (MPO) activity, metallothionein (MT) levels, and histology. Acteoside reduced histological severity scores by 75, 78, and 88% in the duodenum, jejunum, and ileum, respectively, compared to MTX-controls on d 5. Acteoside reduced crypt depth by 49, 51, and 33% and increased villus height by 19, 38, and 10% in the duodenum, jejunum, and ileum, respectively, compared to MTX-controls on d 5. Acteoside decreased MT by 50% compared to MTX-control mice on d 5. Acteoside decreased MPO by 60% and 30% in the duodenum and jejunum, respectively, compared to MTX-controls on d 5. Acteoside alleviated MTX-induced small intestinal mucositis possibly by preventing inflammation.http://dx.doi.org/10.1155/2015/327872 |
| spellingShingle | Daniel Reinke Stamatiki Kritas Panagiotis Polychronopoulos Alexios L. Skaltsounis Nektarios Aligiannis Cuong D. Tran Herbal Substance, Acteoside, Alleviates Intestinal Mucositis in Mice Gastroenterology Research and Practice |
| title | Herbal Substance, Acteoside, Alleviates Intestinal Mucositis in Mice |
| title_full | Herbal Substance, Acteoside, Alleviates Intestinal Mucositis in Mice |
| title_fullStr | Herbal Substance, Acteoside, Alleviates Intestinal Mucositis in Mice |
| title_full_unstemmed | Herbal Substance, Acteoside, Alleviates Intestinal Mucositis in Mice |
| title_short | Herbal Substance, Acteoside, Alleviates Intestinal Mucositis in Mice |
| title_sort | herbal substance acteoside alleviates intestinal mucositis in mice |
| url | http://dx.doi.org/10.1155/2015/327872 |
| work_keys_str_mv | AT danielreinke herbalsubstanceacteosidealleviatesintestinalmucositisinmice AT stamatikikritas herbalsubstanceacteosidealleviatesintestinalmucositisinmice AT panagiotispolychronopoulos herbalsubstanceacteosidealleviatesintestinalmucositisinmice AT alexioslskaltsounis herbalsubstanceacteosidealleviatesintestinalmucositisinmice AT nektariosaligiannis herbalsubstanceacteosidealleviatesintestinalmucositisinmice AT cuongdtran herbalsubstanceacteosidealleviatesintestinalmucositisinmice |