Phenotypic and molecular analysis of Mexican Metarhizium anisopliae strains
Metarhizium anisopliae is an anamorphic fungus widely studied due to its use as a biological control agent. In Mexico, it is mainly used as an integrated management strategy to control populations of spittlebugs (Hemiptera: Cercopidae) that affect sugarcane fields and cattle pastures. In this study...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidad Nacional Autónoma de México
2019-03-01
|
| Series: | Revista Mexicana de Biodiversidad |
| Subjects: | |
| Online Access: | https://revista.ib.unam.mx/index.php/bio/article/view/2643 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850278438197264384 |
|---|---|
| author | Hortensia Navarro-Barranco Carolina Brunner-Mendoza María del Rocío Reyes-Montes Esperanza Duarte-Escalante Conchita Toriello |
| author_facet | Hortensia Navarro-Barranco Carolina Brunner-Mendoza María del Rocío Reyes-Montes Esperanza Duarte-Escalante Conchita Toriello |
| author_sort | Hortensia Navarro-Barranco |
| collection | DOAJ |
| description |
Metarhizium anisopliae is an anamorphic fungus widely studied due to its use as a biological control agent. In Mexico, it is mainly used as an integrated management strategy to control populations of spittlebugs (Hemiptera: Cercopidae) that affect sugarcane fields and cattle pastures. In this study, 13 strains isolated from Aeneolamia spp. (Hemiptera: Cercopidae) from the main areas of sugarcane production of Mexico and 11 reference strains of Metarhizium from different insect hosts and geographic origins were evaluated based on their conidial dimensions, thermotolerance at 15, 25, 30, and 35 ºC, random amplification of polymorphic DNA (RAPD) patterns using a combined analysis with 14 different oligonucleotides and diversity estimators, and a phylogenetic analysis with the ribosomal RNA internal transcribed spacer (ITS) region. All strains used in this study showed typical morphological characteristics corresponding to M. anisopliae and were able to grow at 25 and 30 ºC with restricted growth at low (15 ºC) and high temperatures (35 ºC). Polymorphism analysis clustered all strains from Aeneolamia sp. in one welldefined group with low variability among them. Phylogenetic relationships based on nuclear ribosomal ITS region sequences recovered a subclade within M. anisopliae formed by 10 of the Mexican strains.
|
| format | Article |
| id | doaj-art-2ab73c0e8dcb48b485e97cc88f7965ac |
| institution | OA Journals |
| issn | 1870-3453 2007-8706 |
| language | English |
| publishDate | 2019-03-01 |
| publisher | Universidad Nacional Autónoma de México |
| record_format | Article |
| series | Revista Mexicana de Biodiversidad |
| spelling | doaj-art-2ab73c0e8dcb48b485e97cc88f7965ac2025-08-20T01:49:31ZengUniversidad Nacional Autónoma de MéxicoRevista Mexicana de Biodiversidad1870-34532007-87062019-03-019010.22201/ib.20078706e.2019.90.2643Phenotypic and molecular analysis of Mexican Metarhizium anisopliae strainsHortensia Navarro-Barranco0Carolina Brunner-Mendoza1María del Rocío Reyes-Montes2Esperanza Duarte-Escalante3Conchita Toriello4Universidad Nacional Autonoma de MexicoUniversidad Nacional Autonoma de MexicoUniversidad Nacional Autonoma de MexicoUniversidad Nacional Autonoma de MexicoUniversidad Nacional Autonoma de Mexico Metarhizium anisopliae is an anamorphic fungus widely studied due to its use as a biological control agent. In Mexico, it is mainly used as an integrated management strategy to control populations of spittlebugs (Hemiptera: Cercopidae) that affect sugarcane fields and cattle pastures. In this study, 13 strains isolated from Aeneolamia spp. (Hemiptera: Cercopidae) from the main areas of sugarcane production of Mexico and 11 reference strains of Metarhizium from different insect hosts and geographic origins were evaluated based on their conidial dimensions, thermotolerance at 15, 25, 30, and 35 ºC, random amplification of polymorphic DNA (RAPD) patterns using a combined analysis with 14 different oligonucleotides and diversity estimators, and a phylogenetic analysis with the ribosomal RNA internal transcribed spacer (ITS) region. All strains used in this study showed typical morphological characteristics corresponding to M. anisopliae and were able to grow at 25 and 30 ºC with restricted growth at low (15 ºC) and high temperatures (35 ºC). Polymorphism analysis clustered all strains from Aeneolamia sp. in one welldefined group with low variability among them. Phylogenetic relationships based on nuclear ribosomal ITS region sequences recovered a subclade within M. anisopliae formed by 10 of the Mexican strains. https://revista.ib.unam.mx/index.php/bio/article/view/2643MetarhiziumSugarcaneCattle pasturesITS regionRAPD |
| spellingShingle | Hortensia Navarro-Barranco Carolina Brunner-Mendoza María del Rocío Reyes-Montes Esperanza Duarte-Escalante Conchita Toriello Phenotypic and molecular analysis of Mexican Metarhizium anisopliae strains Revista Mexicana de Biodiversidad Metarhizium Sugarcane Cattle pastures ITS region RAPD |
| title | Phenotypic and molecular analysis of Mexican Metarhizium anisopliae strains |
| title_full | Phenotypic and molecular analysis of Mexican Metarhizium anisopliae strains |
| title_fullStr | Phenotypic and molecular analysis of Mexican Metarhizium anisopliae strains |
| title_full_unstemmed | Phenotypic and molecular analysis of Mexican Metarhizium anisopliae strains |
| title_short | Phenotypic and molecular analysis of Mexican Metarhizium anisopliae strains |
| title_sort | phenotypic and molecular analysis of mexican metarhizium anisopliae strains |
| topic | Metarhizium Sugarcane Cattle pastures ITS region RAPD |
| url | https://revista.ib.unam.mx/index.php/bio/article/view/2643 |
| work_keys_str_mv | AT hortensianavarrobarranco phenotypicandmolecularanalysisofmexicanmetarhiziumanisopliaestrains AT carolinabrunnermendoza phenotypicandmolecularanalysisofmexicanmetarhiziumanisopliaestrains AT mariadelrocioreyesmontes phenotypicandmolecularanalysisofmexicanmetarhiziumanisopliaestrains AT esperanzaduarteescalante phenotypicandmolecularanalysisofmexicanmetarhiziumanisopliaestrains AT conchitatoriello phenotypicandmolecularanalysisofmexicanmetarhiziumanisopliaestrains |