Perturbed gut microbiota and serum metabolites are associated with progressive renal fibrosis
IntroductionThe intricate pathogenesis of renal fibrosis necessitates identifying biomarkers at various stages to facilitate targeted therapeutic interventions, which would enhance patient survival rates and significantly improve prognosis.MethodsWe investigated the changes in gut microbiota and ser...
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Frontiers Media S.A.
2025-04-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2025.1489100/full |
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| author | Run-Xi Wang Hong-Bing Zhou Hong-Bing Zhou Jia-Xing Gao Xing-Hua Li Wan-Fu Bai Jia Wang Ying-Chun Bai Li-Ya Fan Hong Chang Song-Li Shi Song-Li Shi |
| author_facet | Run-Xi Wang Hong-Bing Zhou Hong-Bing Zhou Jia-Xing Gao Xing-Hua Li Wan-Fu Bai Jia Wang Ying-Chun Bai Li-Ya Fan Hong Chang Song-Li Shi Song-Li Shi |
| author_sort | Run-Xi Wang |
| collection | DOAJ |
| description | IntroductionThe intricate pathogenesis of renal fibrosis necessitates identifying biomarkers at various stages to facilitate targeted therapeutic interventions, which would enhance patient survival rates and significantly improve prognosis.MethodsWe investigated the changes in gut microbiota and serum metabolites during the early, middle, and late stages of renal fibrosis in rats using 16S rDNA sequencing and UPLC-QTOF/MS-based metabolomics.ResultsWe identified 5, 21, and 14 potential gut microbial markers and 19, 23, and 31 potential metabolic markers in the MOD1, MOD2, and MOD4 groups, respectively. Bifidobacterium was identified as a shared microbial marker between the MOD1 and MOD2 groups; Prevotellaceae_NK3B31_group and Bacteroides were identified as shared microbial markers between the MOD2 and MOD4 groups. The pathways of arachidonic acid metabolism and retinol metabolism were found to play a significant role in the modulation of renal fibrosis at 1, 2, and 4 weeks. Notably, the metabolic biomarkers 8,9-EET and 5(S)-HPETE within these pathways emerged as critical determinants influencing renal fibrosis.DiscussionOur findings demonstrated that the severity of renal fibrosis is associated with dysbiosis of the gut microbiota and alterations in serum metabolites. |
| format | Article |
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| institution | DOAJ |
| issn | 2296-858X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Medicine |
| spelling | doaj-art-2aa4dcc296e644fda8e0022436ffbaa52025-08-20T03:19:02ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-04-011210.3389/fmed.2025.14891001489100Perturbed gut microbiota and serum metabolites are associated with progressive renal fibrosisRun-Xi Wang0Hong-Bing Zhou1Hong-Bing Zhou2Jia-Xing Gao3Xing-Hua Li4Wan-Fu Bai5Jia Wang6Ying-Chun Bai7Li-Ya Fan8Hong Chang9Song-Li Shi10Song-Li Shi11Department of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, ChinaDepartment of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, ChinaInstitute of Bioactive Substance and Function of Chinese Materia Medica and Mongolian Medicine, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, ChinaDepartment of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, ChinaChangzhi People’s Hospital Affiliated to Changzhi Medical College, Changzhi, ChinaDepartment of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, ChinaDepartment of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, ChinaDepartment of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, ChinaDepartment of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, ChinaDepartment of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, ChinaDepartment of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, ChinaInstitute of Bioactive Substance and Function of Chinese Materia Medica and Mongolian Medicine, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, ChinaIntroductionThe intricate pathogenesis of renal fibrosis necessitates identifying biomarkers at various stages to facilitate targeted therapeutic interventions, which would enhance patient survival rates and significantly improve prognosis.MethodsWe investigated the changes in gut microbiota and serum metabolites during the early, middle, and late stages of renal fibrosis in rats using 16S rDNA sequencing and UPLC-QTOF/MS-based metabolomics.ResultsWe identified 5, 21, and 14 potential gut microbial markers and 19, 23, and 31 potential metabolic markers in the MOD1, MOD2, and MOD4 groups, respectively. Bifidobacterium was identified as a shared microbial marker between the MOD1 and MOD2 groups; Prevotellaceae_NK3B31_group and Bacteroides were identified as shared microbial markers between the MOD2 and MOD4 groups. The pathways of arachidonic acid metabolism and retinol metabolism were found to play a significant role in the modulation of renal fibrosis at 1, 2, and 4 weeks. Notably, the metabolic biomarkers 8,9-EET and 5(S)-HPETE within these pathways emerged as critical determinants influencing renal fibrosis.DiscussionOur findings demonstrated that the severity of renal fibrosis is associated with dysbiosis of the gut microbiota and alterations in serum metabolites.https://www.frontiersin.org/articles/10.3389/fmed.2025.1489100/fullchronic kidney diseaserenal fibrosisdisease progressionoxidative stressinflammationbiochemical markers |
| spellingShingle | Run-Xi Wang Hong-Bing Zhou Hong-Bing Zhou Jia-Xing Gao Xing-Hua Li Wan-Fu Bai Jia Wang Ying-Chun Bai Li-Ya Fan Hong Chang Song-Li Shi Song-Li Shi Perturbed gut microbiota and serum metabolites are associated with progressive renal fibrosis Frontiers in Medicine chronic kidney disease renal fibrosis disease progression oxidative stress inflammation biochemical markers |
| title | Perturbed gut microbiota and serum metabolites are associated with progressive renal fibrosis |
| title_full | Perturbed gut microbiota and serum metabolites are associated with progressive renal fibrosis |
| title_fullStr | Perturbed gut microbiota and serum metabolites are associated with progressive renal fibrosis |
| title_full_unstemmed | Perturbed gut microbiota and serum metabolites are associated with progressive renal fibrosis |
| title_short | Perturbed gut microbiota and serum metabolites are associated with progressive renal fibrosis |
| title_sort | perturbed gut microbiota and serum metabolites are associated with progressive renal fibrosis |
| topic | chronic kidney disease renal fibrosis disease progression oxidative stress inflammation biochemical markers |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2025.1489100/full |
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