Evaluation of Strawberry <i>Colletotrichum</i> spp. Genetic Diversity in Lithuania
Strawberry anthracnose is becoming more important from a scientific and economic standpoint. The <i>Colletotrichum</i> spp. pathogen complex includes <i>C. fragariae, C. gloeosporioides</i> and <i>C. acutatum</i>. The aim was to use microsatellite (SSR) markers to...
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2025-03-01
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| author | Armina Morkeliūnė Neringa Rasiukevičiūtė Birutė Frercks Vidmantas Bendokas Raminta Antanynienė Ingrida Mažeikienė Viktorija Vaštakaitė-Kairienė Rasa Karklelienė Alma Valiuškaitė |
| author_facet | Armina Morkeliūnė Neringa Rasiukevičiūtė Birutė Frercks Vidmantas Bendokas Raminta Antanynienė Ingrida Mažeikienė Viktorija Vaštakaitė-Kairienė Rasa Karklelienė Alma Valiuškaitė |
| author_sort | Armina Morkeliūnė |
| collection | DOAJ |
| description | Strawberry anthracnose is becoming more important from a scientific and economic standpoint. The <i>Colletotrichum</i> spp. pathogen complex includes <i>C. fragariae, C. gloeosporioides</i> and <i>C. acutatum</i>. The aim was to use microsatellite (SSR) markers to assess the genetic diversity of <i>Colletotrichum</i> species. We used seven SSR primer pairs previously developed for the <i>C. acutatum</i> (3) and <i>C. gloeosporioides</i> (4) species. To analyze the genetic diversity of <i>C. fragariae</i>, it was discovered that SSR primer pairs created for <i>C. gloeosporioides</i> were helpful. SSR molecular markers were used in this study for the first time to identify the species of <i>C. fragariae</i>. The average polymorphism information content (PIC) value across all SSR primer pairs was 0.72, making them all informative. The most informative SSR primers were CG22 and CG30, with PIC values of 0.83 and 0.82, respectively. We believe these primers are suitable for the genetic diversity analysis of <i>C. fragariae</i> species. Therefore, the SSR primer pairs CG20 and CG30 are suggested for the genetic investigation of <i>C. acutatum</i> and <i>C. gloeosporioides</i>. A higher incidence of <i>Colletotrichum</i> spp. polymorphism in Lithuania can be linked to adaptation to survival in our environment, according to this study’s findings on the number of alleles and the degree of genetic diversity, which are higher than the results reported in the literature. |
| format | Article |
| id | doaj-art-2a9d4660c05e42068b0b2b3dbc1a75c9 |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Agronomy |
| spelling | doaj-art-2a9d4660c05e42068b0b2b3dbc1a75c92025-08-20T03:40:43ZengMDPI AGAgronomy2073-43952025-03-0115372010.3390/agronomy15030720Evaluation of Strawberry <i>Colletotrichum</i> spp. Genetic Diversity in LithuaniaArmina Morkeliūnė0Neringa Rasiukevičiūtė1Birutė Frercks2Vidmantas Bendokas3Raminta Antanynienė4Ingrida Mažeikienė5Viktorija Vaštakaitė-Kairienė6Rasa Karklelienė7Alma Valiuškaitė8Laboratory of Plant Protection, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas District, LT-54333 Babtai, LithuaniaLaboratory of Plant Protection, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas District, LT-54333 Babtai, LithuaniaDepartment of Orchard Plant Genetics and Biotechnology, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas District, LT-54333 Babtai, LithuaniaDepartment of Orchard Plant Genetics and Biotechnology, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas District, LT-54333 Babtai, LithuaniaDepartment of Orchard Plant Genetics and Biotechnology, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas District, LT-54333 Babtai, LithuaniaDepartment of Orchard Plant Genetics and Biotechnology, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas District, LT-54333 Babtai, LithuaniaLaboratory of Plant Protection, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas District, LT-54333 Babtai, LithuaniaDepartment of Vegetable Breeding and Technology, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas District, LT-54333 Babtai, LithuaniaLaboratory of Plant Protection, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas District, LT-54333 Babtai, LithuaniaStrawberry anthracnose is becoming more important from a scientific and economic standpoint. The <i>Colletotrichum</i> spp. pathogen complex includes <i>C. fragariae, C. gloeosporioides</i> and <i>C. acutatum</i>. The aim was to use microsatellite (SSR) markers to assess the genetic diversity of <i>Colletotrichum</i> species. We used seven SSR primer pairs previously developed for the <i>C. acutatum</i> (3) and <i>C. gloeosporioides</i> (4) species. To analyze the genetic diversity of <i>C. fragariae</i>, it was discovered that SSR primer pairs created for <i>C. gloeosporioides</i> were helpful. SSR molecular markers were used in this study for the first time to identify the species of <i>C. fragariae</i>. The average polymorphism information content (PIC) value across all SSR primer pairs was 0.72, making them all informative. The most informative SSR primers were CG22 and CG30, with PIC values of 0.83 and 0.82, respectively. We believe these primers are suitable for the genetic diversity analysis of <i>C. fragariae</i> species. Therefore, the SSR primer pairs CG20 and CG30 are suggested for the genetic investigation of <i>C. acutatum</i> and <i>C. gloeosporioides</i>. A higher incidence of <i>Colletotrichum</i> spp. polymorphism in Lithuania can be linked to adaptation to survival in our environment, according to this study’s findings on the number of alleles and the degree of genetic diversity, which are higher than the results reported in the literature.https://www.mdpi.com/2073-4395/15/3/720<i>C. gloeosporioides</i><i>C. fragariae</i><i>C. acutatum</i>SSRPIC |
| spellingShingle | Armina Morkeliūnė Neringa Rasiukevičiūtė Birutė Frercks Vidmantas Bendokas Raminta Antanynienė Ingrida Mažeikienė Viktorija Vaštakaitė-Kairienė Rasa Karklelienė Alma Valiuškaitė Evaluation of Strawberry <i>Colletotrichum</i> spp. Genetic Diversity in Lithuania Agronomy <i>C. gloeosporioides</i> <i>C. fragariae</i> <i>C. acutatum</i> SSR PIC |
| title | Evaluation of Strawberry <i>Colletotrichum</i> spp. Genetic Diversity in Lithuania |
| title_full | Evaluation of Strawberry <i>Colletotrichum</i> spp. Genetic Diversity in Lithuania |
| title_fullStr | Evaluation of Strawberry <i>Colletotrichum</i> spp. Genetic Diversity in Lithuania |
| title_full_unstemmed | Evaluation of Strawberry <i>Colletotrichum</i> spp. Genetic Diversity in Lithuania |
| title_short | Evaluation of Strawberry <i>Colletotrichum</i> spp. Genetic Diversity in Lithuania |
| title_sort | evaluation of strawberry i colletotrichum i spp genetic diversity in lithuania |
| topic | <i>C. gloeosporioides</i> <i>C. fragariae</i> <i>C. acutatum</i> SSR PIC |
| url | https://www.mdpi.com/2073-4395/15/3/720 |
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