Evaluation of the Effect of Adipokinetic Hormone/Corazonin-Related Peptide (ACP) on Ovarian Development in the Mud Crab, <i>Scylla paramamosain</i>
In this study, we identified <i>Sp-ACP</i> and its putative receptor from the mud crab <i>S. paramamosain</i> and explored its potential role in ovarian development. RT-PCR results suggested <i>Sp-ACP</i> was extensively expressed in nervous tissues, the ovary, th...
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2024-12-01
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| author | Wenting Tan Yiwei Tang Fang Liu Li Lu An Liu Haihui Ye |
| author_facet | Wenting Tan Yiwei Tang Fang Liu Li Lu An Liu Haihui Ye |
| author_sort | Wenting Tan |
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| description | In this study, we identified <i>Sp-ACP</i> and its putative receptor from the mud crab <i>S. paramamosain</i> and explored its potential role in ovarian development. RT-PCR results suggested <i>Sp-ACP</i> was extensively expressed in nervous tissues, the ovary, the middle gut, and the Y-organ, while <i>Sp-ACPR</i> was highly expressed in the ovary. The expression level of <i>Sp-ACP</i> in the ovary, eyestalk, and cerebral ganglia gradually increased during ovarian development, whereas its receptor exhibited an opposite expression pattern in the ovary. Immunofluorescence revealed that ACP was specifically localized in the follicle cells of the ovary. In vitro experiments showed that the expression of vitellogenin receptor (<i>Sp-VgR</i>) in the ovary was significantly increased by 4 and 6 h incubation of <i>Sp</i>-ACP (10 nM). In addition, 12 h injection of <i>Sp</i>-ACP significantly induced the levels of <i>Sp-Vg</i> in the hepatopancreas and <i>Sp-VgR</i> in the ovary, and hemolymph 17β-estradiol titer. Finally, it demonstrated that prolonged injection of <i>Sp</i>-ACP significantly increased the level of <i>Vg</i> and <i>VgR</i> expression, hemolymph 17β-estradiol titer, GSI, and the oocyte diameter. In conclusion, our results suggested that ACP is involved in the regulation of ovarian development of <i>S. paramamosain</i>, likely by inducing hepatopancreas <i>Sp-Vg</i> expression through estradiol and promoting the uptake of <i>Vg</i> by oocytes. |
| format | Article |
| id | doaj-art-2a9c968957be40c7a223ab54a40c12cc |
| institution | DOAJ |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-2a9c968957be40c7a223ab54a40c12cc2025-08-20T02:56:05ZengMDPI AGAnimals2076-26152024-12-011424370610.3390/ani14243706Evaluation of the Effect of Adipokinetic Hormone/Corazonin-Related Peptide (ACP) on Ovarian Development in the Mud Crab, <i>Scylla paramamosain</i>Wenting Tan0Yiwei Tang1Fang Liu2Li Lu3An Liu4Haihui Ye5State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, ChinaState Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, ChinaState Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, ChinaState Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, ChinaState Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, ChinaState Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen 361021, ChinaIn this study, we identified <i>Sp-ACP</i> and its putative receptor from the mud crab <i>S. paramamosain</i> and explored its potential role in ovarian development. RT-PCR results suggested <i>Sp-ACP</i> was extensively expressed in nervous tissues, the ovary, the middle gut, and the Y-organ, while <i>Sp-ACPR</i> was highly expressed in the ovary. The expression level of <i>Sp-ACP</i> in the ovary, eyestalk, and cerebral ganglia gradually increased during ovarian development, whereas its receptor exhibited an opposite expression pattern in the ovary. Immunofluorescence revealed that ACP was specifically localized in the follicle cells of the ovary. In vitro experiments showed that the expression of vitellogenin receptor (<i>Sp-VgR</i>) in the ovary was significantly increased by 4 and 6 h incubation of <i>Sp</i>-ACP (10 nM). In addition, 12 h injection of <i>Sp</i>-ACP significantly induced the levels of <i>Sp-Vg</i> in the hepatopancreas and <i>Sp-VgR</i> in the ovary, and hemolymph 17β-estradiol titer. Finally, it demonstrated that prolonged injection of <i>Sp</i>-ACP significantly increased the level of <i>Vg</i> and <i>VgR</i> expression, hemolymph 17β-estradiol titer, GSI, and the oocyte diameter. In conclusion, our results suggested that ACP is involved in the regulation of ovarian development of <i>S. paramamosain</i>, likely by inducing hepatopancreas <i>Sp-Vg</i> expression through estradiol and promoting the uptake of <i>Vg</i> by oocytes.https://www.mdpi.com/2076-2615/14/24/3706GnRH-likeadipokinetic hormone/corazonin-related peptideovarian developmentreproductionmud crab |
| spellingShingle | Wenting Tan Yiwei Tang Fang Liu Li Lu An Liu Haihui Ye Evaluation of the Effect of Adipokinetic Hormone/Corazonin-Related Peptide (ACP) on Ovarian Development in the Mud Crab, <i>Scylla paramamosain</i> Animals GnRH-like adipokinetic hormone/corazonin-related peptide ovarian development reproduction mud crab |
| title | Evaluation of the Effect of Adipokinetic Hormone/Corazonin-Related Peptide (ACP) on Ovarian Development in the Mud Crab, <i>Scylla paramamosain</i> |
| title_full | Evaluation of the Effect of Adipokinetic Hormone/Corazonin-Related Peptide (ACP) on Ovarian Development in the Mud Crab, <i>Scylla paramamosain</i> |
| title_fullStr | Evaluation of the Effect of Adipokinetic Hormone/Corazonin-Related Peptide (ACP) on Ovarian Development in the Mud Crab, <i>Scylla paramamosain</i> |
| title_full_unstemmed | Evaluation of the Effect of Adipokinetic Hormone/Corazonin-Related Peptide (ACP) on Ovarian Development in the Mud Crab, <i>Scylla paramamosain</i> |
| title_short | Evaluation of the Effect of Adipokinetic Hormone/Corazonin-Related Peptide (ACP) on Ovarian Development in the Mud Crab, <i>Scylla paramamosain</i> |
| title_sort | evaluation of the effect of adipokinetic hormone corazonin related peptide acp on ovarian development in the mud crab i scylla paramamosain i |
| topic | GnRH-like adipokinetic hormone/corazonin-related peptide ovarian development reproduction mud crab |
| url | https://www.mdpi.com/2076-2615/14/24/3706 |
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