A minimal model for the mitochondrial rapid mode of Ca²+ uptake mechanism.

Mitochondria possess a remarkable ability to rapidly accumulate and sequester Ca²⁺. One of the mechanisms responsible for this ability is believed to be the rapid mode (RaM) of Ca²⁺ uptake. Despite the existence of many models of mitochondrial Ca²⁺ dynamics, very few consider RaM as a potential mech...

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Main Authors: Jason N Bazil, Ranjan K Dash
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0021324&type=printable
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author Jason N Bazil
Ranjan K Dash
author_facet Jason N Bazil
Ranjan K Dash
author_sort Jason N Bazil
collection DOAJ
description Mitochondria possess a remarkable ability to rapidly accumulate and sequester Ca²⁺. One of the mechanisms responsible for this ability is believed to be the rapid mode (RaM) of Ca²⁺ uptake. Despite the existence of many models of mitochondrial Ca²⁺ dynamics, very few consider RaM as a potential mechanism that regulates mitochondrial Ca²⁺ dynamics. To fill this gap, a novel mathematical model of the RaM mechanism is developed herein. The model is able to simulate the available experimental data of rapid Ca²⁺ uptake in isolated mitochondria from both chicken heart and rat liver tissues with good fidelity. The mechanism is based on Ca²⁺ binding to an external trigger site(s) and initiating a brief transient of high Ca²⁺ conductivity. It then quickly switches to an inhibited, zero-conductive state until the external Ca²⁺ level is dropped below a critical value (∼100-150 nM). RaM's Ca²⁺- and time-dependent properties make it a unique Ca²⁺ transporter that may be an important means by which mitochondria take up Ca²⁺ in situ and help enable mitochondria to decode cytosolic Ca²⁺ signals. Integrating the developed RaM model into existing models of mitochondrial Ca²⁺ dynamics will help elucidate the physiological role that this unique mechanism plays in mitochondrial Ca²⁺-homeostasis and bioenergetics.
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spelling doaj-art-2a9901592da5435a936c93a67049d4112025-08-20T03:09:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0166e2132410.1371/journal.pone.0021324A minimal model for the mitochondrial rapid mode of Ca²+ uptake mechanism.Jason N BazilRanjan K DashMitochondria possess a remarkable ability to rapidly accumulate and sequester Ca²⁺. One of the mechanisms responsible for this ability is believed to be the rapid mode (RaM) of Ca²⁺ uptake. Despite the existence of many models of mitochondrial Ca²⁺ dynamics, very few consider RaM as a potential mechanism that regulates mitochondrial Ca²⁺ dynamics. To fill this gap, a novel mathematical model of the RaM mechanism is developed herein. The model is able to simulate the available experimental data of rapid Ca²⁺ uptake in isolated mitochondria from both chicken heart and rat liver tissues with good fidelity. The mechanism is based on Ca²⁺ binding to an external trigger site(s) and initiating a brief transient of high Ca²⁺ conductivity. It then quickly switches to an inhibited, zero-conductive state until the external Ca²⁺ level is dropped below a critical value (∼100-150 nM). RaM's Ca²⁺- and time-dependent properties make it a unique Ca²⁺ transporter that may be an important means by which mitochondria take up Ca²⁺ in situ and help enable mitochondria to decode cytosolic Ca²⁺ signals. Integrating the developed RaM model into existing models of mitochondrial Ca²⁺ dynamics will help elucidate the physiological role that this unique mechanism plays in mitochondrial Ca²⁺-homeostasis and bioenergetics.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0021324&type=printable
spellingShingle Jason N Bazil
Ranjan K Dash
A minimal model for the mitochondrial rapid mode of Ca²+ uptake mechanism.
PLoS ONE
title A minimal model for the mitochondrial rapid mode of Ca²+ uptake mechanism.
title_full A minimal model for the mitochondrial rapid mode of Ca²+ uptake mechanism.
title_fullStr A minimal model for the mitochondrial rapid mode of Ca²+ uptake mechanism.
title_full_unstemmed A minimal model for the mitochondrial rapid mode of Ca²+ uptake mechanism.
title_short A minimal model for the mitochondrial rapid mode of Ca²+ uptake mechanism.
title_sort minimal model for the mitochondrial rapid mode of ca² uptake mechanism
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0021324&type=printable
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