Markers for Characterization of Bone Marrow Multipotential Stromal Cells
Given the observed efficacy of culture-expanded multipotential stromal cells, also termed mesenchymal stem cells (MSCs), in the treatment of graft-versus host and cardiac disease, it remains surprising that purity and potency characterization of manufactured cell batches remains rather basic. In thi...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2012/975871 |
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| author | Sally A. Boxall Elena Jones |
| author_facet | Sally A. Boxall Elena Jones |
| author_sort | Sally A. Boxall |
| collection | DOAJ |
| description | Given the observed efficacy of culture-expanded multipotential stromal cells, also termed mesenchymal stem cells (MSCs), in the treatment of graft-versus host and cardiac disease, it remains surprising that purity and potency characterization of manufactured cell batches remains rather basic. In this paper, we will initially discuss surface and molecular markers that were proposed to serve as the indicators of the MSC potency, in terms of their proliferative potential or the ability to differentiate into desired lineages. The second part of this paper will be dedicated to a critical discussion of surface markers of uncultured (i.e., native) bone marrow (BM) MSCs. Although no formal consensus has yet been reached on which markers may be best suited for prospective BM MSC isolation, markers that cross-react with MSCs of animal models (such as CD271 and W8-B2/MSCA-1) may have the strongest translational value. Whereas small animal models are needed to discover the in vivo function on these markers, large animal models are required for safety and efficacy testing of isolated MSCs, particularly in the field of bone and cartilage tissue engineering. |
| format | Article |
| id | doaj-art-2a989c50ae9a43af8cfa049bf56aadf7 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-2a989c50ae9a43af8cfa049bf56aadf72025-08-20T03:54:43ZengWileyStem Cells International1687-966X1687-96782012-01-01201210.1155/2012/975871975871Markers for Characterization of Bone Marrow Multipotential Stromal CellsSally A. Boxall0Elena Jones1Academic Unit of the Musculoskeletal Diseases, St. James's University Hospital, Leeds Institute of Molecular Medicine, University of Leeds, Leeds LS9 7TF, UKAcademic Unit of the Musculoskeletal Diseases, St. James's University Hospital, Leeds Institute of Molecular Medicine, University of Leeds, Leeds LS9 7TF, UKGiven the observed efficacy of culture-expanded multipotential stromal cells, also termed mesenchymal stem cells (MSCs), in the treatment of graft-versus host and cardiac disease, it remains surprising that purity and potency characterization of manufactured cell batches remains rather basic. In this paper, we will initially discuss surface and molecular markers that were proposed to serve as the indicators of the MSC potency, in terms of their proliferative potential or the ability to differentiate into desired lineages. The second part of this paper will be dedicated to a critical discussion of surface markers of uncultured (i.e., native) bone marrow (BM) MSCs. Although no formal consensus has yet been reached on which markers may be best suited for prospective BM MSC isolation, markers that cross-react with MSCs of animal models (such as CD271 and W8-B2/MSCA-1) may have the strongest translational value. Whereas small animal models are needed to discover the in vivo function on these markers, large animal models are required for safety and efficacy testing of isolated MSCs, particularly in the field of bone and cartilage tissue engineering.http://dx.doi.org/10.1155/2012/975871 |
| spellingShingle | Sally A. Boxall Elena Jones Markers for Characterization of Bone Marrow Multipotential Stromal Cells Stem Cells International |
| title | Markers for Characterization of Bone Marrow Multipotential Stromal Cells |
| title_full | Markers for Characterization of Bone Marrow Multipotential Stromal Cells |
| title_fullStr | Markers for Characterization of Bone Marrow Multipotential Stromal Cells |
| title_full_unstemmed | Markers for Characterization of Bone Marrow Multipotential Stromal Cells |
| title_short | Markers for Characterization of Bone Marrow Multipotential Stromal Cells |
| title_sort | markers for characterization of bone marrow multipotential stromal cells |
| url | http://dx.doi.org/10.1155/2012/975871 |
| work_keys_str_mv | AT sallyaboxall markersforcharacterizationofbonemarrowmultipotentialstromalcells AT elenajones markersforcharacterizationofbonemarrowmultipotentialstromalcells |