Promising Noninvasive Cellular Phenotype in Prostate Cancer Cells Knockdown of Matrix Metalloproteinase 9
Cell surface interaction of CD44 and MMP9 increases migration and invasion of PC3 cells. We show here that stable knockdown of MMP9 in PC3 cells switches CD44 isoform expression from CD44s to CD44v6 which is more glycosylated. These cells showed highly adhesive morphology with extensive cell spreadi...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/493689 |
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| author | Aditi Gupta Wei Cao Kavitha Sadashivaiah Wantao Chen Abraham Schneider Meenakshi A. Chellaiah |
| author_facet | Aditi Gupta Wei Cao Kavitha Sadashivaiah Wantao Chen Abraham Schneider Meenakshi A. Chellaiah |
| author_sort | Aditi Gupta |
| collection | DOAJ |
| description | Cell surface interaction of CD44 and MMP9 increases migration and invasion of PC3 cells. We show here that stable knockdown of MMP9 in PC3 cells switches CD44 isoform expression from CD44s to CD44v6 which is more glycosylated. These cells showed highly adhesive morphology with extensive cell spreading which is due to the formation of focal adhesions and well organized actin-stress fibers. MMP9 knockdown blocks invadopodia formation and matrix degradation activity as well. However, CD44 knockdown PC3 cells failed to develop focal adhesions and stress fibers; hence these cells make unstable adhesions. A part of the reason for these changes could be caused by silencing of CD44v6 as well. Immunostaining of prostate tissue microarray sections illustrated significantly lower levels of CD44v6 in adenocarcinoma than normal tissue. Our results suggest that interaction between CD44 and MMP9 is a potential mechanism of invadopodia formation. CD44v6 expression may be essential for the protection of non-invasive cellular phenotype. CD44v6 decrease may be a potential marker for prognosis and therapeutics. |
| format | Article |
| id | doaj-art-2a96a4eec16b4e3d9be16777cdb05280 |
| institution | OA Journals |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-2a96a4eec16b4e3d9be16777cdb052802025-08-20T02:04:09ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/493689493689Promising Noninvasive Cellular Phenotype in Prostate Cancer Cells Knockdown of Matrix Metalloproteinase 9Aditi Gupta0Wei Cao1Kavitha Sadashivaiah2Wantao Chen3Abraham Schneider4Meenakshi A. Chellaiah5Department of Oncology and Diagnostic Sciences, School of Dentistry, University of Maryland, Baltimore, MD 21201, USALaboratory of Oral Tumor Biology, Department of Oral and Maxillofacial Surgery, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaDepartment of Oncology and Diagnostic Sciences, School of Dentistry, University of Maryland, Baltimore, MD 21201, USALaboratory of Oral Tumor Biology, Department of Oral and Maxillofacial Surgery, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaDepartment of Oncology and Diagnostic Sciences, School of Dentistry, University of Maryland, Baltimore, MD 21201, USADepartment of Oncology and Diagnostic Sciences, School of Dentistry, University of Maryland, Baltimore, MD 21201, USACell surface interaction of CD44 and MMP9 increases migration and invasion of PC3 cells. We show here that stable knockdown of MMP9 in PC3 cells switches CD44 isoform expression from CD44s to CD44v6 which is more glycosylated. These cells showed highly adhesive morphology with extensive cell spreading which is due to the formation of focal adhesions and well organized actin-stress fibers. MMP9 knockdown blocks invadopodia formation and matrix degradation activity as well. However, CD44 knockdown PC3 cells failed to develop focal adhesions and stress fibers; hence these cells make unstable adhesions. A part of the reason for these changes could be caused by silencing of CD44v6 as well. Immunostaining of prostate tissue microarray sections illustrated significantly lower levels of CD44v6 in adenocarcinoma than normal tissue. Our results suggest that interaction between CD44 and MMP9 is a potential mechanism of invadopodia formation. CD44v6 expression may be essential for the protection of non-invasive cellular phenotype. CD44v6 decrease may be a potential marker for prognosis and therapeutics.http://dx.doi.org/10.1155/2013/493689 |
| spellingShingle | Aditi Gupta Wei Cao Kavitha Sadashivaiah Wantao Chen Abraham Schneider Meenakshi A. Chellaiah Promising Noninvasive Cellular Phenotype in Prostate Cancer Cells Knockdown of Matrix Metalloproteinase 9 The Scientific World Journal |
| title | Promising Noninvasive Cellular Phenotype in Prostate Cancer Cells Knockdown of Matrix Metalloproteinase 9 |
| title_full | Promising Noninvasive Cellular Phenotype in Prostate Cancer Cells Knockdown of Matrix Metalloproteinase 9 |
| title_fullStr | Promising Noninvasive Cellular Phenotype in Prostate Cancer Cells Knockdown of Matrix Metalloproteinase 9 |
| title_full_unstemmed | Promising Noninvasive Cellular Phenotype in Prostate Cancer Cells Knockdown of Matrix Metalloproteinase 9 |
| title_short | Promising Noninvasive Cellular Phenotype in Prostate Cancer Cells Knockdown of Matrix Metalloproteinase 9 |
| title_sort | promising noninvasive cellular phenotype in prostate cancer cells knockdown of matrix metalloproteinase 9 |
| url | http://dx.doi.org/10.1155/2013/493689 |
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