An androgen reduced transcript of LncRNA GAS5 promoted prostate cancer proliferation.

Prostate cancer (PCa) becomes a leading cause of death in males nowadays. Recent reports showed that androgen-responsive long non-coding RNAs played important roles in tumorigenesis and progression of PCa. In this study, we focused on a special transcript of GAS5 (ENST00000456293.5, GAS5-007), which...

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Main Authors: Yingyi Zhang, Xinya Su, Zhe Kong, Fangqiu Fu, Pu Zhang, Dan Wang, Hai Wu, Xuechao Wan, Yao Li
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182305&type=printable
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author Yingyi Zhang
Xinya Su
Zhe Kong
Fangqiu Fu
Pu Zhang
Dan Wang
Hai Wu
Xuechao Wan
Yao Li
author_facet Yingyi Zhang
Xinya Su
Zhe Kong
Fangqiu Fu
Pu Zhang
Dan Wang
Hai Wu
Xuechao Wan
Yao Li
author_sort Yingyi Zhang
collection DOAJ
description Prostate cancer (PCa) becomes a leading cause of death in males nowadays. Recent reports showed that androgen-responsive long non-coding RNAs played important roles in tumorigenesis and progression of PCa. In this study, we focused on a special transcript of GAS5 (ENST00000456293.5, GAS5-007), which was reported as a tumor suppressor. Here, we demonstrated GAS5-007 was reduced by androgen treatment and inhibited by AR. Next, we explored the expression level of GAS, finding the expression of it in PCa tissue was higher than normal tissue in both public databases and human tissue samples. Functional analysis of GAS5 showed it was related to regulating translational elongation, protein biosynthesis, and transcription. Moreover, we observed GAS5-007 knockdown inhibited the proliferation, cell cycle and promoted cell apoptosis of PCa. We also constructed a GAS5-miRNA network to explain the different roles of different GAS5 transcripts in PCa. This study provides novel insights to identify potential diagnostic biomarker and therapy target for prostate cancer in clinical treatment.
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publisher Public Library of Science (PLoS)
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spelling doaj-art-2a95f39aaa344f2786fc4aeca9daca992025-08-20T03:12:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018230510.1371/journal.pone.0182305An androgen reduced transcript of LncRNA GAS5 promoted prostate cancer proliferation.Yingyi ZhangXinya SuZhe KongFangqiu FuPu ZhangDan WangHai WuXuechao WanYao LiProstate cancer (PCa) becomes a leading cause of death in males nowadays. Recent reports showed that androgen-responsive long non-coding RNAs played important roles in tumorigenesis and progression of PCa. In this study, we focused on a special transcript of GAS5 (ENST00000456293.5, GAS5-007), which was reported as a tumor suppressor. Here, we demonstrated GAS5-007 was reduced by androgen treatment and inhibited by AR. Next, we explored the expression level of GAS, finding the expression of it in PCa tissue was higher than normal tissue in both public databases and human tissue samples. Functional analysis of GAS5 showed it was related to regulating translational elongation, protein biosynthesis, and transcription. Moreover, we observed GAS5-007 knockdown inhibited the proliferation, cell cycle and promoted cell apoptosis of PCa. We also constructed a GAS5-miRNA network to explain the different roles of different GAS5 transcripts in PCa. This study provides novel insights to identify potential diagnostic biomarker and therapy target for prostate cancer in clinical treatment.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182305&type=printable
spellingShingle Yingyi Zhang
Xinya Su
Zhe Kong
Fangqiu Fu
Pu Zhang
Dan Wang
Hai Wu
Xuechao Wan
Yao Li
An androgen reduced transcript of LncRNA GAS5 promoted prostate cancer proliferation.
PLoS ONE
title An androgen reduced transcript of LncRNA GAS5 promoted prostate cancer proliferation.
title_full An androgen reduced transcript of LncRNA GAS5 promoted prostate cancer proliferation.
title_fullStr An androgen reduced transcript of LncRNA GAS5 promoted prostate cancer proliferation.
title_full_unstemmed An androgen reduced transcript of LncRNA GAS5 promoted prostate cancer proliferation.
title_short An androgen reduced transcript of LncRNA GAS5 promoted prostate cancer proliferation.
title_sort androgen reduced transcript of lncrna gas5 promoted prostate cancer proliferation
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182305&type=printable
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