In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) Plant
Erythrina plants, locally known as “dadap ayam”, are higher plant species and have been used as a folk medicine for treatment of cancer. To prove the effectiveness of the leaves and stem bark of E. variegata as an anti-cancer agent, the assay in this research was focused on in vitro test towards bre...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indonesian Society for Cancer Chemoprevention
2011-10-01
|
| Series: | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| Online Access: | https://ijcc.chemoprev.org/index.php/ijcc/article/view/44 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849323369320677376 |
|---|---|
| author | Tati Herlina Unang Supratman Anas Subarnas Supriyatna Sutardjo Suseno Amien Hideo Hayashi |
| author_facet | Tati Herlina Unang Supratman Anas Subarnas Supriyatna Sutardjo Suseno Amien Hideo Hayashi |
| author_sort | Tati Herlina |
| collection | DOAJ |
| description | Erythrina plants, locally known as “dadap ayam”, are higher plant species and have been used as a folk medicine for treatment of cancer. To prove the effectiveness of the leaves and stem bark of E. variegata as an anti-cancer agent, the assay in this research was focused on in vitro test towards breast cancer cell T47D. In the course of our continuing search for novel anti-cancer agent from Erythrina plants, the methanol extract of the leaves and stem bark of E. variegata showed significant anti-cancer activity against breast cancer cell T47D in vitro using the Sulphorhodamine B (SRB) assay. By using the anti-cancer activity to follow the separations, the methanol extract was separated by combination of column chromatography. The chemical structure of an anti-cancer compounds were determined on the basis of spectroscopic evidence and comparison with the previously reported and identified as an erythrina alkaloid (1) and isoflavonoid (2). Compounds (1-2) showed anti-cancer activity against breast cancer cell T47D used with IC50 of 1.0 and 3.3 µg/mL, respectively. This results strongly suggested that E. variegata is promising sources for anti-cancer agents.
Keywords: Anti-cancer, Erythrina variegata, Leguminoseae |
| format | Article |
| id | doaj-art-2a9035e9257b4d9ea39eac458cb77dec |
| institution | Kabale University |
| issn | 2088-0197 2355-8989 |
| language | English |
| publishDate | 2011-10-01 |
| publisher | Indonesian Society for Cancer Chemoprevention |
| record_format | Article |
| series | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| spelling | doaj-art-2a9035e9257b4d9ea39eac458cb77dec2025-08-20T03:49:03ZengIndonesian Society for Cancer ChemopreventionISCC (Indonesian Journal of Cancer Chemoprevention)2088-01972355-89892011-10-012328629010.14499/indonesianjcanchemoprev2iss3pp286-29045In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) PlantTati Herlina0Unang Supratman1Anas Subarnas2Supriyatna Sutardjo3Suseno Amien4Hideo Hayashi5Departement of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas PadjadjaranDepartement of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas PadjadjaranFaculty of Pharmacy, Univesitas PadjadjaranFaculty of Pharmacy, Universitas PadjadjaranFaculty of Agriculture, Universitas PadjadjaranLaboratory of Natural Products Chemistry, Division of Applied Biological Chemistry, Graduate School of Agriculture and Life Science, Osaka Prefecture UniversityErythrina plants, locally known as “dadap ayam”, are higher plant species and have been used as a folk medicine for treatment of cancer. To prove the effectiveness of the leaves and stem bark of E. variegata as an anti-cancer agent, the assay in this research was focused on in vitro test towards breast cancer cell T47D. In the course of our continuing search for novel anti-cancer agent from Erythrina plants, the methanol extract of the leaves and stem bark of E. variegata showed significant anti-cancer activity against breast cancer cell T47D in vitro using the Sulphorhodamine B (SRB) assay. By using the anti-cancer activity to follow the separations, the methanol extract was separated by combination of column chromatography. The chemical structure of an anti-cancer compounds were determined on the basis of spectroscopic evidence and comparison with the previously reported and identified as an erythrina alkaloid (1) and isoflavonoid (2). Compounds (1-2) showed anti-cancer activity against breast cancer cell T47D used with IC50 of 1.0 and 3.3 µg/mL, respectively. This results strongly suggested that E. variegata is promising sources for anti-cancer agents. Keywords: Anti-cancer, Erythrina variegata, Leguminoseaehttps://ijcc.chemoprev.org/index.php/ijcc/article/view/44 |
| spellingShingle | Tati Herlina Unang Supratman Anas Subarnas Supriyatna Sutardjo Suseno Amien Hideo Hayashi In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) Plant ISCC (Indonesian Journal of Cancer Chemoprevention) |
| title | In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) Plant |
| title_full | In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) Plant |
| title_fullStr | In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) Plant |
| title_full_unstemmed | In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) Plant |
| title_short | In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) Plant |
| title_sort | in vitro anti cancer alkaloid and flavonoid extracted from the erythrina variegata leguminoseae plant |
| url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/44 |
| work_keys_str_mv | AT tatiherlina invitroanticanceralkaloidandflavonoidextractedfromtheerythrinavariegataleguminoseaeplant AT unangsupratman invitroanticanceralkaloidandflavonoidextractedfromtheerythrinavariegataleguminoseaeplant AT anassubarnas invitroanticanceralkaloidandflavonoidextractedfromtheerythrinavariegataleguminoseaeplant AT supriyatnasutardjo invitroanticanceralkaloidandflavonoidextractedfromtheerythrinavariegataleguminoseaeplant AT susenoamien invitroanticanceralkaloidandflavonoidextractedfromtheerythrinavariegataleguminoseaeplant AT hideohayashi invitroanticanceralkaloidandflavonoidextractedfromtheerythrinavariegataleguminoseaeplant |