Specificity and tunability of efflux pumps: A new role for the proton gradient?
Efflux pumps that transport antibacterial drugs out of bacterial cells have broad specificity, commonly leading to broad spectrum resistance and limiting treatment strategies for infections. It remains unclear how efflux pumps can maintain this broad spectrum specificity to diverse drug molecules wh...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS Computational Biology |
| Online Access: | https://doi.org/10.1371/journal.pcbi.1012772 |
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| author | Matthew Gerry Duncan Kirby Boian S Alexandrov Dvira Segal Anton Zilman |
| author_facet | Matthew Gerry Duncan Kirby Boian S Alexandrov Dvira Segal Anton Zilman |
| author_sort | Matthew Gerry |
| collection | DOAJ |
| description | Efflux pumps that transport antibacterial drugs out of bacterial cells have broad specificity, commonly leading to broad spectrum resistance and limiting treatment strategies for infections. It remains unclear how efflux pumps can maintain this broad spectrum specificity to diverse drug molecules while limiting the efflux of other cytoplasmic content. We have investigated the origins of this broad specificity using theoretical models informed by the experimentally determined structural and kinetic properties of efflux pumps. We developed a set of mathematical models describing operation of efflux pumps as a discrete cyclic stochastic process across a network of states characterizing pump conformations and the presence/absence of bound ligands and protons. These include a minimal three-state model that lends itself to clear analytic calculations as well as a five-state model that relaxes some of the simpler model's most strict assumptions. We found that the pump specificity is determined not solely by the drug affinity to the pump-as is commonly assumed-but it is also directly affected by the periplasmic pH and the transmembrane potential. Therefore, changes to the proton concentration gradient and voltage drop across the membrane can influence how effective the pump is at extruding a particular drug molecule. Furthermore, we found that while both the proton concentration gradient across the membrane and the transmembrane potential contribute to the thermodynamic force driving the pump, their effects on the efflux enter not strictly in a combined proton motive force. Rather, they have two distinguishable effects on the overall throughput. These results highlight the unexpected effects of thermodynamic driving forces out of equilibrium and illustrate how efflux pump structure and function are conducive to the emergence of multidrug resistance. |
| format | Article |
| id | doaj-art-2a8f689989714eab8c48641980a409fd |
| institution | Kabale University |
| issn | 1553-734X 1553-7358 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj-art-2a8f689989714eab8c48641980a409fd2025-02-12T05:30:33ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582025-01-01211e101277210.1371/journal.pcbi.1012772Specificity and tunability of efflux pumps: A new role for the proton gradient?Matthew GerryDuncan KirbyBoian S AlexandrovDvira SegalAnton ZilmanEfflux pumps that transport antibacterial drugs out of bacterial cells have broad specificity, commonly leading to broad spectrum resistance and limiting treatment strategies for infections. It remains unclear how efflux pumps can maintain this broad spectrum specificity to diverse drug molecules while limiting the efflux of other cytoplasmic content. We have investigated the origins of this broad specificity using theoretical models informed by the experimentally determined structural and kinetic properties of efflux pumps. We developed a set of mathematical models describing operation of efflux pumps as a discrete cyclic stochastic process across a network of states characterizing pump conformations and the presence/absence of bound ligands and protons. These include a minimal three-state model that lends itself to clear analytic calculations as well as a five-state model that relaxes some of the simpler model's most strict assumptions. We found that the pump specificity is determined not solely by the drug affinity to the pump-as is commonly assumed-but it is also directly affected by the periplasmic pH and the transmembrane potential. Therefore, changes to the proton concentration gradient and voltage drop across the membrane can influence how effective the pump is at extruding a particular drug molecule. Furthermore, we found that while both the proton concentration gradient across the membrane and the transmembrane potential contribute to the thermodynamic force driving the pump, their effects on the efflux enter not strictly in a combined proton motive force. Rather, they have two distinguishable effects on the overall throughput. These results highlight the unexpected effects of thermodynamic driving forces out of equilibrium and illustrate how efflux pump structure and function are conducive to the emergence of multidrug resistance.https://doi.org/10.1371/journal.pcbi.1012772 |
| spellingShingle | Matthew Gerry Duncan Kirby Boian S Alexandrov Dvira Segal Anton Zilman Specificity and tunability of efflux pumps: A new role for the proton gradient? PLoS Computational Biology |
| title | Specificity and tunability of efflux pumps: A new role for the proton gradient? |
| title_full | Specificity and tunability of efflux pumps: A new role for the proton gradient? |
| title_fullStr | Specificity and tunability of efflux pumps: A new role for the proton gradient? |
| title_full_unstemmed | Specificity and tunability of efflux pumps: A new role for the proton gradient? |
| title_short | Specificity and tunability of efflux pumps: A new role for the proton gradient? |
| title_sort | specificity and tunability of efflux pumps a new role for the proton gradient |
| url | https://doi.org/10.1371/journal.pcbi.1012772 |
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