Integrating physiological and transcriptomic analyses at the single-neuron level
Neurons generate various spike patterns to execute different functions. Understanding how these physiological neuronal spike patterns are related to their molecular characteristics is a long-standing issue in neuroscience. Herein, we review the results of recent studies that have addressed this issu...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-05-01
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| Series: | Neuroscience Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0168010224000658 |
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| author | Haruya Yagishita Takuya Sasaki |
| author_facet | Haruya Yagishita Takuya Sasaki |
| author_sort | Haruya Yagishita |
| collection | DOAJ |
| description | Neurons generate various spike patterns to execute different functions. Understanding how these physiological neuronal spike patterns are related to their molecular characteristics is a long-standing issue in neuroscience. Herein, we review the results of recent studies that have addressed this issue by integrating physiological and transcriptomic techniques. A sequence of experiments, including in vivo recording and/or labeling, brain tissue slicing, cell collection, and transcriptomic analysis, have identified the gene expression profiles of brain neurons at the single-cell level, with activity patterns recorded in living animals. Although these techniques are still in the early stages, this methodological idea is principally applicable to various brain regions and neuronal activity patterns. Accumulating evidence will contribute to a deeper understanding of neuronal characteristics by integrating insights from molecules to cells, circuits, and behaviors. |
| format | Article |
| id | doaj-art-2a86d3f9c5e8483fadd9c8b7a8d1a86b |
| institution | OA Journals |
| issn | 0168-0102 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neuroscience Research |
| spelling | doaj-art-2a86d3f9c5e8483fadd9c8b7a8d1a86b2025-08-20T02:30:15ZengElsevierNeuroscience Research0168-01022025-05-01214697410.1016/j.neures.2024.05.003Integrating physiological and transcriptomic analyses at the single-neuron levelHaruya Yagishita0Takuya Sasaki1Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aramaki-Aoba, Aoba-Ku, Sendai 980-8578, JapanDepartment of Pharmacology, Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aramaki-Aoba, Aoba-Ku, Sendai 980-8578, Japan; Corresponding author.Neurons generate various spike patterns to execute different functions. Understanding how these physiological neuronal spike patterns are related to their molecular characteristics is a long-standing issue in neuroscience. Herein, we review the results of recent studies that have addressed this issue by integrating physiological and transcriptomic techniques. A sequence of experiments, including in vivo recording and/or labeling, brain tissue slicing, cell collection, and transcriptomic analysis, have identified the gene expression profiles of brain neurons at the single-cell level, with activity patterns recorded in living animals. Although these techniques are still in the early stages, this methodological idea is principally applicable to various brain regions and neuronal activity patterns. Accumulating evidence will contribute to a deeper understanding of neuronal characteristics by integrating insights from molecules to cells, circuits, and behaviors.http://www.sciencedirect.com/science/article/pii/S0168010224000658ElectrophysiologyImagingSpikeRNA-seqSpatial transcriptome |
| spellingShingle | Haruya Yagishita Takuya Sasaki Integrating physiological and transcriptomic analyses at the single-neuron level Neuroscience Research Electrophysiology Imaging Spike RNA-seq Spatial transcriptome |
| title | Integrating physiological and transcriptomic analyses at the single-neuron level |
| title_full | Integrating physiological and transcriptomic analyses at the single-neuron level |
| title_fullStr | Integrating physiological and transcriptomic analyses at the single-neuron level |
| title_full_unstemmed | Integrating physiological and transcriptomic analyses at the single-neuron level |
| title_short | Integrating physiological and transcriptomic analyses at the single-neuron level |
| title_sort | integrating physiological and transcriptomic analyses at the single neuron level |
| topic | Electrophysiology Imaging Spike RNA-seq Spatial transcriptome |
| url | http://www.sciencedirect.com/science/article/pii/S0168010224000658 |
| work_keys_str_mv | AT haruyayagishita integratingphysiologicalandtranscriptomicanalysesatthesingleneuronlevel AT takuyasasaki integratingphysiologicalandtranscriptomicanalysesatthesingleneuronlevel |