Respiratory microbiome and metabolome features associate disease severity and the need for doxycycline treatment in children with macrolide-resistant Mycoplasma pneumoniae-mediated pneumonia
IntroductionCommensal bacterial community along the upper respiratory tract functions against pathogens. The host determinants of Mycoplasma pneumoniae severity should be identified against the increasing threat of macrolide-resistant M. pneumoniae (MRMP) infection. We hypothesized that respiratory...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1537182/full |
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| author | Wei-Chao Liao Wei-Chao Liao Shiao-Wen Li En-Wei Hsing Sung-Han Hsiao Ian Yi-Feng Chang Yin-Cheng Chen Yi-Yin Chen Yi-Jiun Pan Yu-Chia Hsieh |
| author_facet | Wei-Chao Liao Wei-Chao Liao Shiao-Wen Li En-Wei Hsing Sung-Han Hsiao Ian Yi-Feng Chang Yin-Cheng Chen Yi-Yin Chen Yi-Jiun Pan Yu-Chia Hsieh |
| author_sort | Wei-Chao Liao |
| collection | DOAJ |
| description | IntroductionCommensal bacterial community along the upper respiratory tract functions against pathogens. The host determinants of Mycoplasma pneumoniae severity should be identified against the increasing threat of macrolide-resistant M. pneumoniae (MRMP) infection. We hypothesized that respiratory microbiome is involved in the clinical manifestations of M. pneumoniae infection.MethodsFrom 2017 to 2020, 92 children with MRMP-mediated pneumonia were enrolled among 845 children with community-associated pneumonia. Oropharyngeal samplings were collected within 48 h after admission. We compared respiratory microbiome and metabolites based on patients’ later development of prolonged fever and the need for doxycycline treatment (DT, n = 57) and the cured control without fever or doxycycline treatment (WDT, n = 35) by using 16S rRNA-based sequencing and untargeted metabolome analysis.ResultsSignificantly higher diversity and different respiratory microbiomes were evaluated in WDT patients in contrast to DT patients. Fusobacterium, Haemophilus, Gemella, Oribacterium, Actinomyces lingnae, Fusobacterium periodonticum, Gemella sanguinis, and Solobacterium moorei were inversely correlated with disease severity. We assumed that metabolites of divergent microbiomes were related to MRMP development. We identified 15 discriminative amino-acid- and fatty-acid-related metabolites in two groups. F. periodonticum abundance was negatively associated with an inflammatory metabolite: a platelet-activating factor. Fusobacterium and Oribacterium were related to the decrease in LysoPE(18:1(9Z)/0:0) and LysoPC(18:1(9Z)).ConclusionsMicrobiota dysbiosis with dysregulated inflammatory glycerolphospholipid-related metabolites was related to disease severity and the need for doxycycline treatment in children with MRMP-mediated pneumonia. Anaerobic bacteria metabolites and metabolic pathway could be beneficial therapeutic targets against M. pneumoniae infection. |
| format | Article |
| id | doaj-art-2a84ff8859714ddc83a80f91d996ed4f |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-2a84ff8859714ddc83a80f91d996ed4f2025-08-20T03:34:30ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-07-011510.3389/fcimb.2025.15371821537182Respiratory microbiome and metabolome features associate disease severity and the need for doxycycline treatment in children with macrolide-resistant Mycoplasma pneumoniae-mediated pneumoniaWei-Chao Liao0Wei-Chao Liao1Shiao-Wen Li2En-Wei Hsing3Sung-Han Hsiao4Ian Yi-Feng Chang5Yin-Cheng Chen6Yi-Yin Chen7Yi-Jiun Pan8Yu-Chia Hsieh9Molecular Medicine Research Center, Chang Gung University, Taoyuan, TaiwanDepartment of Pediatrics, Chang Gung Children’s Hospital, Chang Gung Memorial Hospital, Chang Gung University, College of Medicine, Taoyuan, TaiwanDepartment of Life Sciences, National University of Kaohsiung, Kaohsiung, TaiwanDepartment of Pediatrics, Chang Gung Children’s Hospital, Chang Gung Memorial Hospital, Chang Gung University, College of Medicine, Taoyuan, TaiwanDivision of Infectious Diseases, Department of Medicine, Chang Gung Memorial Hospital, Taoyuan, TaiwanMolecular Medicine Research Center, Chang Gung University, Taoyuan, TaiwanInstitute of BioMedical Informatics, National Yang Ming Chiao Tung University, Taipei, TaiwanGraduate Institute of Immunology, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Microbiology and Immunology, School of Medicine, College of Medicine, China Medical University, Taichung, TaiwanDepartment of Pediatrics, Chang Gung Children’s Hospital, Chang Gung Memorial Hospital, Chang Gung University, College of Medicine, Taoyuan, TaiwanIntroductionCommensal bacterial community along the upper respiratory tract functions against pathogens. The host determinants of Mycoplasma pneumoniae severity should be identified against the increasing threat of macrolide-resistant M. pneumoniae (MRMP) infection. We hypothesized that respiratory microbiome is involved in the clinical manifestations of M. pneumoniae infection.MethodsFrom 2017 to 2020, 92 children with MRMP-mediated pneumonia were enrolled among 845 children with community-associated pneumonia. Oropharyngeal samplings were collected within 48 h after admission. We compared respiratory microbiome and metabolites based on patients’ later development of prolonged fever and the need for doxycycline treatment (DT, n = 57) and the cured control without fever or doxycycline treatment (WDT, n = 35) by using 16S rRNA-based sequencing and untargeted metabolome analysis.ResultsSignificantly higher diversity and different respiratory microbiomes were evaluated in WDT patients in contrast to DT patients. Fusobacterium, Haemophilus, Gemella, Oribacterium, Actinomyces lingnae, Fusobacterium periodonticum, Gemella sanguinis, and Solobacterium moorei were inversely correlated with disease severity. We assumed that metabolites of divergent microbiomes were related to MRMP development. We identified 15 discriminative amino-acid- and fatty-acid-related metabolites in two groups. F. periodonticum abundance was negatively associated with an inflammatory metabolite: a platelet-activating factor. Fusobacterium and Oribacterium were related to the decrease in LysoPE(18:1(9Z)/0:0) and LysoPC(18:1(9Z)).ConclusionsMicrobiota dysbiosis with dysregulated inflammatory glycerolphospholipid-related metabolites was related to disease severity and the need for doxycycline treatment in children with MRMP-mediated pneumonia. Anaerobic bacteria metabolites and metabolic pathway could be beneficial therapeutic targets against M. pneumoniae infection.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1537182/fullMycoplasma pneumoniaepneumoniarespiratorymicrobiomemetabolomemacrolide-resistant Mycoplasma pneumoniae |
| spellingShingle | Wei-Chao Liao Wei-Chao Liao Shiao-Wen Li En-Wei Hsing Sung-Han Hsiao Ian Yi-Feng Chang Yin-Cheng Chen Yi-Yin Chen Yi-Jiun Pan Yu-Chia Hsieh Respiratory microbiome and metabolome features associate disease severity and the need for doxycycline treatment in children with macrolide-resistant Mycoplasma pneumoniae-mediated pneumonia Frontiers in Cellular and Infection Microbiology Mycoplasma pneumoniae pneumonia respiratory microbiome metabolome macrolide-resistant Mycoplasma pneumoniae |
| title | Respiratory microbiome and metabolome features associate disease severity and the need for doxycycline treatment in children with macrolide-resistant Mycoplasma pneumoniae-mediated pneumonia |
| title_full | Respiratory microbiome and metabolome features associate disease severity and the need for doxycycline treatment in children with macrolide-resistant Mycoplasma pneumoniae-mediated pneumonia |
| title_fullStr | Respiratory microbiome and metabolome features associate disease severity and the need for doxycycline treatment in children with macrolide-resistant Mycoplasma pneumoniae-mediated pneumonia |
| title_full_unstemmed | Respiratory microbiome and metabolome features associate disease severity and the need for doxycycline treatment in children with macrolide-resistant Mycoplasma pneumoniae-mediated pneumonia |
| title_short | Respiratory microbiome and metabolome features associate disease severity and the need for doxycycline treatment in children with macrolide-resistant Mycoplasma pneumoniae-mediated pneumonia |
| title_sort | respiratory microbiome and metabolome features associate disease severity and the need for doxycycline treatment in children with macrolide resistant mycoplasma pneumoniae mediated pneumonia |
| topic | Mycoplasma pneumoniae pneumonia respiratory microbiome metabolome macrolide-resistant Mycoplasma pneumoniae |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1537182/full |
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