Genetic Variants Linked with the Concentration of Sex Hormone-Binding Globulin Correlate with Uterine Fibroid Risk

In this study we searched for correlations between polymorphic variants that determine sex hormone-binding globulin concentration (SHBG<sub>con</sub>) and uterine fibroids (UFs). The work was performed on a sample of 1542 women (569 with UFs and 973 without UFs [control]), from whom we o...

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Main Authors: Marina Ponomarenko, Evgeny Reshetnikov, Maria Churnosova, Inna Aristova, Maria Abramova, Vitaly Novakov, Vladimir Churnosov, Alexey Polonikov, Denis Plotnikov, Mikhail Churnosov, Irina Ponomarenko
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Language:English
Published: MDPI AG 2025-07-01
Series:Life
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Online Access:https://www.mdpi.com/2075-1729/15/7/1150
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author Marina Ponomarenko
Evgeny Reshetnikov
Maria Churnosova
Inna Aristova
Maria Abramova
Vitaly Novakov
Vladimir Churnosov
Alexey Polonikov
Denis Plotnikov
Mikhail Churnosov
Irina Ponomarenko
author_facet Marina Ponomarenko
Evgeny Reshetnikov
Maria Churnosova
Inna Aristova
Maria Abramova
Vitaly Novakov
Vladimir Churnosov
Alexey Polonikov
Denis Plotnikov
Mikhail Churnosov
Irina Ponomarenko
author_sort Marina Ponomarenko
collection DOAJ
description In this study we searched for correlations between polymorphic variants that determine sex hormone-binding globulin concentration (SHBG<sub>con</sub>) and uterine fibroids (UFs). The work was performed on a sample of 1542 women (569 with UFs and 973 without UFs [control]), from whom we obtained experimental data on the distribution of nine single-nucleotide polymorphisms (SNPs) affecting the SHBG<sub>con</sub> (data confirmed in genome-wide association studies [GWASs]). When searching for associations with UFs, both the independent effects of SNPs and the effects of their SNP–SNP interactions (SNP-SNP<sub>ints</sub>) were taken into account during the “deep study” of the functionality of seven important UF loci and 115 strongly linked [r<sup>2</sup> ≥ 0.80] variants (an in silico methodology was used). As the results show, two SHBG<sub>con</sub>-related SNPs correlated with UF risk: rs3779195 [T/A] <i>BAIAP2L1</i> (OR<sub>AA</sub> = 0.38; 95%CI<sub>AA</sub> = 0.20–0.91; p<sub>perm(AA)</sub> = 0.023) and rs440837 [A/G] <i>ZBTB10</i> (OR<sub>GG</sub> = 1.93; 95%CI<sub>GG</sub> = 1.17–3.14; p<sub>perm(GG)</sub> = 0.010). At the same time, seven SHBG<sub>con</sub>-related SNPs interacting with each other (four models of such SNP-SNP<sub>ints</sub> [p<sub>perm</sub> ≤ 0.01)] were found to influence UF risk. These SHBG<sub>con</sub>-related SNPs, determining susceptibility to UF, showed strong functional relevance and were involved in pathways of gene transcription regulation, interactions with hormone ligand-binding receptors, the content control of SHBG, testosterone, liver enzymes, lipids, etc. This study’s results demonstrate the effect of significant SHBG<sub>con</sub>-related genetic determinants of UF risk.
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spelling doaj-art-2a65002cc3344462a490d900f3980b402025-08-20T03:08:09ZengMDPI AGLife2075-17292025-07-01157115010.3390/life15071150Genetic Variants Linked with the Concentration of Sex Hormone-Binding Globulin Correlate with Uterine Fibroid RiskMarina Ponomarenko0Evgeny Reshetnikov1Maria Churnosova2Inna Aristova3Maria Abramova4Vitaly Novakov5Vladimir Churnosov6Alexey Polonikov7Denis Plotnikov8Mikhail Churnosov9Irina Ponomarenko10Department of Medical Biological Disciplines, Belgorod State National Research University, 308015 Belgorod, RussiaDepartment of Medical Biological Disciplines, Belgorod State National Research University, 308015 Belgorod, RussiaDepartment of Medical Biological Disciplines, Belgorod State National Research University, 308015 Belgorod, RussiaDepartment of Medical Biological Disciplines, Belgorod State National Research University, 308015 Belgorod, RussiaDepartment of Medical Biological Disciplines, Belgorod State National Research University, 308015 Belgorod, RussiaDepartment of Medical Biological Disciplines, Belgorod State National Research University, 308015 Belgorod, RussiaDepartment of Medical Biological Disciplines, Belgorod State National Research University, 308015 Belgorod, RussiaDepartment of Medical Biological Disciplines, Belgorod State National Research University, 308015 Belgorod, RussiaGenetic Epidemiology Lab, Kazan State Medical University, 420012 Kazan, RussiaDepartment of Medical Biological Disciplines, Belgorod State National Research University, 308015 Belgorod, RussiaDepartment of Medical Biological Disciplines, Belgorod State National Research University, 308015 Belgorod, RussiaIn this study we searched for correlations between polymorphic variants that determine sex hormone-binding globulin concentration (SHBG<sub>con</sub>) and uterine fibroids (UFs). The work was performed on a sample of 1542 women (569 with UFs and 973 without UFs [control]), from whom we obtained experimental data on the distribution of nine single-nucleotide polymorphisms (SNPs) affecting the SHBG<sub>con</sub> (data confirmed in genome-wide association studies [GWASs]). When searching for associations with UFs, both the independent effects of SNPs and the effects of their SNP–SNP interactions (SNP-SNP<sub>ints</sub>) were taken into account during the “deep study” of the functionality of seven important UF loci and 115 strongly linked [r<sup>2</sup> ≥ 0.80] variants (an in silico methodology was used). As the results show, two SHBG<sub>con</sub>-related SNPs correlated with UF risk: rs3779195 [T/A] <i>BAIAP2L1</i> (OR<sub>AA</sub> = 0.38; 95%CI<sub>AA</sub> = 0.20–0.91; p<sub>perm(AA)</sub> = 0.023) and rs440837 [A/G] <i>ZBTB10</i> (OR<sub>GG</sub> = 1.93; 95%CI<sub>GG</sub> = 1.17–3.14; p<sub>perm(GG)</sub> = 0.010). At the same time, seven SHBG<sub>con</sub>-related SNPs interacting with each other (four models of such SNP-SNP<sub>ints</sub> [p<sub>perm</sub> ≤ 0.01)] were found to influence UF risk. These SHBG<sub>con</sub>-related SNPs, determining susceptibility to UF, showed strong functional relevance and were involved in pathways of gene transcription regulation, interactions with hormone ligand-binding receptors, the content control of SHBG, testosterone, liver enzymes, lipids, etc. This study’s results demonstrate the effect of significant SHBG<sub>con</sub>-related genetic determinants of UF risk.https://www.mdpi.com/2075-1729/15/7/1150associationsex hormone-binding globulinSNPuterine fibroids
spellingShingle Marina Ponomarenko
Evgeny Reshetnikov
Maria Churnosova
Inna Aristova
Maria Abramova
Vitaly Novakov
Vladimir Churnosov
Alexey Polonikov
Denis Plotnikov
Mikhail Churnosov
Irina Ponomarenko
Genetic Variants Linked with the Concentration of Sex Hormone-Binding Globulin Correlate with Uterine Fibroid Risk
Life
association
sex hormone-binding globulin
SNP
uterine fibroids
title Genetic Variants Linked with the Concentration of Sex Hormone-Binding Globulin Correlate with Uterine Fibroid Risk
title_full Genetic Variants Linked with the Concentration of Sex Hormone-Binding Globulin Correlate with Uterine Fibroid Risk
title_fullStr Genetic Variants Linked with the Concentration of Sex Hormone-Binding Globulin Correlate with Uterine Fibroid Risk
title_full_unstemmed Genetic Variants Linked with the Concentration of Sex Hormone-Binding Globulin Correlate with Uterine Fibroid Risk
title_short Genetic Variants Linked with the Concentration of Sex Hormone-Binding Globulin Correlate with Uterine Fibroid Risk
title_sort genetic variants linked with the concentration of sex hormone binding globulin correlate with uterine fibroid risk
topic association
sex hormone-binding globulin
SNP
uterine fibroids
url https://www.mdpi.com/2075-1729/15/7/1150
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