Effect of heat-treatment on microstructure of hydroxyapatite porous microspheres for enhanced biocompatibility

Abstract An in-depth exploration into the impact of heat-treatment on the microstructure of HA microspheres is imperative to enhance their biocompatibility, a prerequisite for the development of high-performance HA biomaterials. In this study, the impact of heat-treatment at 600–1000 °C on the micro...

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Main Authors: Wei-li Xu, Xue-hui Sun, Dong Xue, Ji-zhao Guo, Xin-ke Yan, Pei-jian Sun, Yi Cao, Mei-li Qi, Cong Nie
Format: Article
Language:English
Published: Springer 2025-06-01
Series:Journal of Saudi Chemical Society
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Online Access:https://doi.org/10.1007/s44442-025-00010-4
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author Wei-li Xu
Xue-hui Sun
Dong Xue
Ji-zhao Guo
Xin-ke Yan
Pei-jian Sun
Yi Cao
Mei-li Qi
Cong Nie
author_facet Wei-li Xu
Xue-hui Sun
Dong Xue
Ji-zhao Guo
Xin-ke Yan
Pei-jian Sun
Yi Cao
Mei-li Qi
Cong Nie
author_sort Wei-li Xu
collection DOAJ
description Abstract An in-depth exploration into the impact of heat-treatment on the microstructure of HA microspheres is imperative to enhance their biocompatibility, a prerequisite for the development of high-performance HA biomaterials. In this study, the impact of heat-treatment at 600–1000 °C on the microstructure and biocompatibility of HA porous microspheres prepared by the hydrothermal method was investigated. The results showed that the microsheet-assembled HA porous microspheres with diameters of approximately 40–120 μm were obtained. The crystallinity of HA porous microspheres increased with the rise of heat-treatment temperature while the specific surface area and particle size decreased. SEM and TEM observations indicated that the pore size and through porosity of HA microspheres increased under high temperature conditions based on the mechanism of fusion growth of grains. The CCK-8 assay and Live/Dead staining evaluations revealed that heat-treatment promoted the biocompatibility and bioactivity of HA porous microspheres. This study provides new concepts for the controllable optimization of the microstructure and biological properties of HA materials to broaden their clinical applications.
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institution Kabale University
issn 1319-6103
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language English
publishDate 2025-06-01
publisher Springer
record_format Article
series Journal of Saudi Chemical Society
spelling doaj-art-2a59ca6cb9914d1ba6370a62d7d300c32025-08-20T04:01:47ZengSpringerJournal of Saudi Chemical Society1319-61032212-46402025-06-012931710.1007/s44442-025-00010-4Effect of heat-treatment on microstructure of hydroxyapatite porous microspheres for enhanced biocompatibilityWei-li Xu0Xue-hui Sun1Dong Xue2Ji-zhao Guo3Xin-ke Yan4Pei-jian Sun5Yi Cao6Mei-li Qi7Cong Nie8Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTCKey Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTCChina Tobacco Jiangsu Industrial Co., Ltd.Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTCChina Tobacco Jiangsu Industrial Co., Ltd.Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTCChina Tobacco Jiangsu Industrial Co., Ltd.School of Transportation and Civil Engineering, Shandong Jiaotong UniversityKey Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTCAbstract An in-depth exploration into the impact of heat-treatment on the microstructure of HA microspheres is imperative to enhance their biocompatibility, a prerequisite for the development of high-performance HA biomaterials. In this study, the impact of heat-treatment at 600–1000 °C on the microstructure and biocompatibility of HA porous microspheres prepared by the hydrothermal method was investigated. The results showed that the microsheet-assembled HA porous microspheres with diameters of approximately 40–120 μm were obtained. The crystallinity of HA porous microspheres increased with the rise of heat-treatment temperature while the specific surface area and particle size decreased. SEM and TEM observations indicated that the pore size and through porosity of HA microspheres increased under high temperature conditions based on the mechanism of fusion growth of grains. The CCK-8 assay and Live/Dead staining evaluations revealed that heat-treatment promoted the biocompatibility and bioactivity of HA porous microspheres. This study provides new concepts for the controllable optimization of the microstructure and biological properties of HA materials to broaden their clinical applications.https://doi.org/10.1007/s44442-025-00010-4HydroxyapatiteHeat-treatmentMicrostructureBiocompatibility
spellingShingle Wei-li Xu
Xue-hui Sun
Dong Xue
Ji-zhao Guo
Xin-ke Yan
Pei-jian Sun
Yi Cao
Mei-li Qi
Cong Nie
Effect of heat-treatment on microstructure of hydroxyapatite porous microspheres for enhanced biocompatibility
Journal of Saudi Chemical Society
Hydroxyapatite
Heat-treatment
Microstructure
Biocompatibility
title Effect of heat-treatment on microstructure of hydroxyapatite porous microspheres for enhanced biocompatibility
title_full Effect of heat-treatment on microstructure of hydroxyapatite porous microspheres for enhanced biocompatibility
title_fullStr Effect of heat-treatment on microstructure of hydroxyapatite porous microspheres for enhanced biocompatibility
title_full_unstemmed Effect of heat-treatment on microstructure of hydroxyapatite porous microspheres for enhanced biocompatibility
title_short Effect of heat-treatment on microstructure of hydroxyapatite porous microspheres for enhanced biocompatibility
title_sort effect of heat treatment on microstructure of hydroxyapatite porous microspheres for enhanced biocompatibility
topic Hydroxyapatite
Heat-treatment
Microstructure
Biocompatibility
url https://doi.org/10.1007/s44442-025-00010-4
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