RNAi-Mediated <i>FoxO</i> Silencing Inhibits Reproduction in <i>Locusta migratoria</i>
<i>FoxO</i> is a downstream target gene of cellular nutrient and growth factors, oxidative stress responses, and insulin signaling pathways. It play a crucial role in insect growth, development, and reproduction. <i>Locusta migratoria</i> is a significant agricultural pest; t...
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2024-11-01
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| author | Jiaying Xu Zeming Yuan Huazhang Zhao Xinru Wu Nina Cai Tingting Ma Bin Tang Gongxing Chen Shigui Wang |
| author_facet | Jiaying Xu Zeming Yuan Huazhang Zhao Xinru Wu Nina Cai Tingting Ma Bin Tang Gongxing Chen Shigui Wang |
| author_sort | Jiaying Xu |
| collection | DOAJ |
| description | <i>FoxO</i> is a downstream target gene of cellular nutrient and growth factors, oxidative stress responses, and insulin signaling pathways. It play a crucial role in insect growth, development, and reproduction. <i>Locusta migratoria</i> is a significant agricultural pest; therefore, the identification of novel control targets for its management is of significant importance. After injecting dsRNA to interfere with <i>FoxO</i> expression, we observed changes in the reproduction-related gene expression and ovary development through RT-qPCR and morphological observation. Simultaneously, the trehalose and glycogen contents were measured following RNAi. The results demonstrate that interference with <i>FoxO</i> significantly downregulates key genes in the Hippo pathway and <i>Notch</i> gene expression. In terms of carbohydrate metabolism, the trehalose content decreases significantly while the glycogen content increases markedly after <i>FoxO</i> silencing. Additionally, <i>FoxO</i> silencing considerably inhibits reproductive-related gene expression, resulting in delayed ovarian development. These findings indicate that <i>FoxO</i> regulates <i>L. migratoria</i> reproduction through the Hippo signaling pathway: when impaired, the reproductive capacity function declines. In addition, <i>FoxO</i>-mediated energy mobilization is involved in the regulation of egg production. These results indicate that the RNAi of <i>FoxO</i> may be a useful control strategy against <i>L. migratoria</i>. |
| format | Article |
| id | doaj-art-2a58e4f2567f4f7a9728abe7141069f5 |
| institution | OA Journals |
| issn | 2075-4450 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj-art-2a58e4f2567f4f7a9728abe7141069f52025-08-20T02:05:03ZengMDPI AGInsects2075-44502024-11-01151189110.3390/insects15110891RNAi-Mediated <i>FoxO</i> Silencing Inhibits Reproduction in <i>Locusta migratoria</i>Jiaying Xu0Zeming Yuan1Huazhang Zhao2Xinru Wu3Nina Cai4Tingting Ma5Bin Tang6Gongxing Chen7Shigui Wang8College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaSchool of Pharmacy, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China<i>FoxO</i> is a downstream target gene of cellular nutrient and growth factors, oxidative stress responses, and insulin signaling pathways. It play a crucial role in insect growth, development, and reproduction. <i>Locusta migratoria</i> is a significant agricultural pest; therefore, the identification of novel control targets for its management is of significant importance. After injecting dsRNA to interfere with <i>FoxO</i> expression, we observed changes in the reproduction-related gene expression and ovary development through RT-qPCR and morphological observation. Simultaneously, the trehalose and glycogen contents were measured following RNAi. The results demonstrate that interference with <i>FoxO</i> significantly downregulates key genes in the Hippo pathway and <i>Notch</i> gene expression. In terms of carbohydrate metabolism, the trehalose content decreases significantly while the glycogen content increases markedly after <i>FoxO</i> silencing. Additionally, <i>FoxO</i> silencing considerably inhibits reproductive-related gene expression, resulting in delayed ovarian development. These findings indicate that <i>FoxO</i> regulates <i>L. migratoria</i> reproduction through the Hippo signaling pathway: when impaired, the reproductive capacity function declines. In addition, <i>FoxO</i>-mediated energy mobilization is involved in the regulation of egg production. These results indicate that the RNAi of <i>FoxO</i> may be a useful control strategy against <i>L. migratoria</i>.https://www.mdpi.com/2075-4450/15/11/891<i>Locusta migratoria</i>RNAi<i>FoxO</i>Hippo pathwayreproduction |
| spellingShingle | Jiaying Xu Zeming Yuan Huazhang Zhao Xinru Wu Nina Cai Tingting Ma Bin Tang Gongxing Chen Shigui Wang RNAi-Mediated <i>FoxO</i> Silencing Inhibits Reproduction in <i>Locusta migratoria</i> Insects <i>Locusta migratoria</i> RNAi <i>FoxO</i> Hippo pathway reproduction |
| title | RNAi-Mediated <i>FoxO</i> Silencing Inhibits Reproduction in <i>Locusta migratoria</i> |
| title_full | RNAi-Mediated <i>FoxO</i> Silencing Inhibits Reproduction in <i>Locusta migratoria</i> |
| title_fullStr | RNAi-Mediated <i>FoxO</i> Silencing Inhibits Reproduction in <i>Locusta migratoria</i> |
| title_full_unstemmed | RNAi-Mediated <i>FoxO</i> Silencing Inhibits Reproduction in <i>Locusta migratoria</i> |
| title_short | RNAi-Mediated <i>FoxO</i> Silencing Inhibits Reproduction in <i>Locusta migratoria</i> |
| title_sort | rnai mediated i foxo i silencing inhibits reproduction in i locusta migratoria i |
| topic | <i>Locusta migratoria</i> RNAi <i>FoxO</i> Hippo pathway reproduction |
| url | https://www.mdpi.com/2075-4450/15/11/891 |
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