RNAi-Mediated <i>FoxO</i> Silencing Inhibits Reproduction in <i>Locusta migratoria</i>

RNAi-Mediated <i>FoxO</i> Silencing Inhibits Reproduction in <i>Locusta migratoria</i>

<i>FoxO</i> is a downstream target gene of cellular nutrient and growth factors, oxidative stress responses, and insulin signaling pathways. It play a crucial role in insect growth, development, and reproduction. <i>Locusta migratoria</i> is a significant agricultural pest; t...

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Bibliographic Details
Main Authors: Jiaying Xu, Zeming Yuan, Huazhang Zhao, Xinru Wu, Nina Cai, Tingting Ma, Bin Tang, Gongxing Chen, Shigui Wang
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Insects
Subjects:
<i>Locusta migratoria</i>
RNAi
<i>FoxO</i>
Hippo pathway
reproduction
Online Access:https://www.mdpi.com/2075-4450/15/11/891
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Summary:<i>FoxO</i> is a downstream target gene of cellular nutrient and growth factors, oxidative stress responses, and insulin signaling pathways. It play a crucial role in insect growth, development, and reproduction. <i>Locusta migratoria</i> is a significant agricultural pest; therefore, the identification of novel control targets for its management is of significant importance. After injecting dsRNA to interfere with <i>FoxO</i> expression, we observed changes in the reproduction-related gene expression and ovary development through RT-qPCR and morphological observation. Simultaneously, the trehalose and glycogen contents were measured following RNAi. The results demonstrate that interference with <i>FoxO</i> significantly downregulates key genes in the Hippo pathway and <i>Notch</i> gene expression. In terms of carbohydrate metabolism, the trehalose content decreases significantly while the glycogen content increases markedly after <i>FoxO</i> silencing. Additionally, <i>FoxO</i> silencing considerably inhibits reproductive-related gene expression, resulting in delayed ovarian development. These findings indicate that <i>FoxO</i> regulates <i>L. migratoria</i> reproduction through the Hippo signaling pathway: when impaired, the reproductive capacity function declines. In addition, <i>FoxO</i>-mediated energy mobilization is involved in the regulation of egg production. These results indicate that the RNAi of <i>FoxO</i> may be a useful control strategy against <i>L. migratoria</i>.
ISSN:2075-4450

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