A non-binding surface demonstrates increased thrombogenicity under plasma-free conditions
Abstract Although the commercially available, non-binding microplate is characterised as a low-fouling material, its suitability for the study of platelet function in basic research has yet to be determined. This study compares the non-binding microplate with microplates made of polypropylene (PP) a...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-06636-z |
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| author | Cezary Watala Boguslawa Luzak Tomasz Przygodzki Magdalena Boncler |
| author_facet | Cezary Watala Boguslawa Luzak Tomasz Przygodzki Magdalena Boncler |
| author_sort | Cezary Watala |
| collection | DOAJ |
| description | Abstract Although the commercially available, non-binding microplate is characterised as a low-fouling material, its suitability for the study of platelet function in basic research has yet to be determined. This study compares the non-binding microplate with microplates made of polypropylene (PP) and polystyrene (PS) in terms of thrombogenicity. The thrombogenicity of the non-binding surface was evaluated using several platelet assays performed on platelet-rich plasma (PRP) and suspensions of isolated platelets obtained from healthy donors. In PRP, the non-binding surface exhibited similar or lower thrombogenicity than the PP and PS surfaces. However, with isolated platelets, it exhibited similar or higher thrombogenicity. A clear asymmetry in platelet adhesion to the polymers was observed depending on the presence of plasma components. Adhesion was barely detectable for PRP on the non-binding surface and for isolated platelets on the PS and PP surfaces. Conversely, pronounced platelet deposition was observed for PRP on the PP and PS surfaces, and for isolated platelets on the non-binding surface. These observations suggest that platelets may interact directly with functional groups on the non-binding surface. Consequently, the non-binding surface could serve as an alternative to conventional polymeric surfaces in the in vitro platelet assays, when plasma is present. |
| format | Article |
| id | doaj-art-2a519893972b48818e98b3ea6c8aaa95 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-2a519893972b48818e98b3ea6c8aaa952025-08-20T04:01:25ZengNature PortfolioScientific Reports2045-23222025-07-0115111210.1038/s41598-025-06636-zA non-binding surface demonstrates increased thrombogenicity under plasma-free conditionsCezary Watala0Boguslawa Luzak1Tomasz Przygodzki2Magdalena Boncler3Department of Haemostasis and Haemostatic Disorders, Chair of Biomedical Sciences, Medical University of LodzDepartment of Haemostasis and Haemostatic Disorders, Chair of Biomedical Sciences, Medical University of LodzDepartment of Haemostasis and Haemostatic Disorders, Chair of Biomedical Sciences, Medical University of LodzDepartment of Haemostasis and Haemostatic Disorders, Chair of Biomedical Sciences, Medical University of LodzAbstract Although the commercially available, non-binding microplate is characterised as a low-fouling material, its suitability for the study of platelet function in basic research has yet to be determined. This study compares the non-binding microplate with microplates made of polypropylene (PP) and polystyrene (PS) in terms of thrombogenicity. The thrombogenicity of the non-binding surface was evaluated using several platelet assays performed on platelet-rich plasma (PRP) and suspensions of isolated platelets obtained from healthy donors. In PRP, the non-binding surface exhibited similar or lower thrombogenicity than the PP and PS surfaces. However, with isolated platelets, it exhibited similar or higher thrombogenicity. A clear asymmetry in platelet adhesion to the polymers was observed depending on the presence of plasma components. Adhesion was barely detectable for PRP on the non-binding surface and for isolated platelets on the PS and PP surfaces. Conversely, pronounced platelet deposition was observed for PRP on the PP and PS surfaces, and for isolated platelets on the non-binding surface. These observations suggest that platelets may interact directly with functional groups on the non-binding surface. Consequently, the non-binding surface could serve as an alternative to conventional polymeric surfaces in the in vitro platelet assays, when plasma is present.https://doi.org/10.1038/s41598-025-06636-zBlood plateletsPlatelet activationCell adhesionPlatelet aggregationPolypropylenesPolystyrenes |
| spellingShingle | Cezary Watala Boguslawa Luzak Tomasz Przygodzki Magdalena Boncler A non-binding surface demonstrates increased thrombogenicity under plasma-free conditions Scientific Reports Blood platelets Platelet activation Cell adhesion Platelet aggregation Polypropylenes Polystyrenes |
| title | A non-binding surface demonstrates increased thrombogenicity under plasma-free conditions |
| title_full | A non-binding surface demonstrates increased thrombogenicity under plasma-free conditions |
| title_fullStr | A non-binding surface demonstrates increased thrombogenicity under plasma-free conditions |
| title_full_unstemmed | A non-binding surface demonstrates increased thrombogenicity under plasma-free conditions |
| title_short | A non-binding surface demonstrates increased thrombogenicity under plasma-free conditions |
| title_sort | non binding surface demonstrates increased thrombogenicity under plasma free conditions |
| topic | Blood platelets Platelet activation Cell adhesion Platelet aggregation Polypropylenes Polystyrenes |
| url | https://doi.org/10.1038/s41598-025-06636-z |
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